The AlkB monooxygenase of Pseudomonas oleovorans. Synthesis, stability and level in recombinant Escherichia coli and the native host

European Journal of Biochemistry

Volumn 244, Issue 2, 1997, Pages 462-470

  Staijen, Ivo E ^a   Hatzimanikatis, Vassily ^a   Witholt, Bernard ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

Alkane monooxygenase; Membrane protein; Pseudomonas oleovorans; Stability; Synthesis

Indexed keywords

RECOMBINANT ENZYME; UNSPECIFIC MONOOXYGENASE;

ARTICLE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; MOLECULAR CLONING; PRIORITY JOURNAL; PSEUDOMONAS;

ESCHERICHIA COLI; PSEUDOMONAS; PSEUDOMONAS OLEOVORANS;

EID: 0031049966     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.00462.x     Document Type: Article

References (51)

1. Bachmann, B. J. (1987) Derivations and genotypes of some mutant derivatives of Escherichia coli K-12, in Escherichia coli and Salmonella typhimurium: cellular and molecular biology (Neidhardt, F. C., Ingraham, J. L., Low, K. B., Magasanik, B., Schaechter, M. & Umbarger, H. E., eds) vol. 2, pp. 1191-1219, American Society for Microbiology, Washington DC.
2. Baptist, J. N., Gholson, R. K. & Coon, M. J. (1963) Hydrocarbon oxidation by a bacterial enzyme system. I. Products of octane oxidation, Biochim. Biophys. Acta 69, 40-47.
3. Benson, S., Oppici, M., Shapiro, J. & Fennewald, M. (1979) Regulation of membrane peptides by the Pseudomonas plasmid alk regulon, J. Bacteriol. 140, 754-762.
4. Boyer, H. W. & Roulland-Dussoix, D. (1969) A complementation analysis of the restriction and modification of DNA in Escherichia coli, J. Mol. Biol. 41, 459-472.
5. Britten, R. J. & McClure, F. T. (1962) The amino acid pool in Escherichia coli, Bacteriol. Rev. 26, 292-335.
6. Chakrabarty, A. M., Chou, G. & Gunsalus, I. C. (1973) Genetic regulation of octane dissimulation plasmids in Pseudomonas, Proc. Natl Acad. Sci. USA 70, 1137-1140.
7. Chen, Q., Janssen, D. B. & Witholt, B. (1995) Growth on octane alters the membrane lipid fatty acids of Pseudomonas oleovorans due to the induction of alkB and synthesis of octanol, J. Bacteriol. 197, 6894-6901.
8. Cohn, M., Cohen, G. N. & Monod, J. (1953) L'effet inhibiteur spécifique de la méthionine dans la formation de la méthionine-synthase chez E. coli, C.R. Acad. Sci. Ser. III Sci. Vie 236, 746-748.
9. de Leij, L., Kingma, J. & Witholt, B. (1978) Insertion of newly synthesized proteins into the outer membrane of Escherichia coli, Biochim. Biophys. Acta 512, 365-376.
10. Eggink, G., Lageveen, R. G., Altenburg, B. & Witholt, B. (1987) Controlled and functional expression of the Pseudomonas oleovorans alkane utilizing system in Pseudomonas putida and Escherichia coli, J. Biol. Chem. 262, 17712-17718.
11. Eggink, G., Engel, H., Vriend, G., Terpstra, P. & Witholt, B. (1990) Rubredoxin reductase of Pseudomonas oleovorans: structural relationship to other flavoprotein oxidoreductases based on one NAD and two FAD fingerprints, J. Mol. Biol. 212, 135-142.
12. Favre-Bulle, O. & Witholt, B. (1992) Biooxidation of n-octane by a recombinant Escherichia coli in a two-liquid-phase system: effect of medium components on cell growth and alkane oxidation activity, Enzyme Microb. Technol. 14, 931-937.
13. Favre-Bulle, O., Weenink, E., Vos, T., Preusting, H. & Witholt, B. (1992) Continuous bioconversion of n-octane to octanoic acid by recombinant Escherichia coli (alk+) growing in a two-liquid phase chemostat, Biotechnol. Bioeng. 41, 263-272.
14. Fennewald, M. & Shapiro, J. (1977) Regulatory mutations of the Pseudomonas plasmid alk regulon, J. Bacteriol. 132, 622-627.
15. Figurski, D., Meyer, R. & Helinski, D. R. (1979) Suppression of ColE1 replication properties by the Inc P-1 plasmid RK2 in hybrid plasmids constructed in vitro, J. Mol. Biol. 133, 295-318.
16. Frederickson, A. G. (1976) Formulation of structured growth models, Biotechnol. Bioeng. 28, 1481-1488.
17. Goff, S. A. & Goldberg, A. L. (1985) Production of abnormal proteins in E. coli stimulates transcription of lon and other heat shock proteins, Cell 41, 587-595.
18. Gottesman, S. (1987) Regulation by proteolysis, in Escherichia coli and Salmonella typhimurium: cellular and molecular biology (Neidhardt, F. C., Ingraham, J. L., Low, K. B., Magasanik, B., Schaechter, M. & Umbarger, H. E., eds) vol. 2, pp. 1308-1312, American Society for Microbiology, Washington DC.
19. Grund, A., Shapiro, J., Fennewald, M., Bacha, P., Leahy, J., Markbreiter, K., Nieder, M. & Toepfer, M. (1975) Regulation of alkane oxidation in Pseudomonas putida, J. Bacteriol. 123, 546-556.
20. Hansen, J. B. & Olsen, R. H. (1978) The lncP2 Group of Pseudomonas, a class of uniquely large plasmids, Nature 274, 715-717.
21. Harcum, S. W. & Bentley, W. E. (1993) Response dynamics of 26-, 34-, 39-, 54- and 80-kDa proteases in induced cultures of recombinant Escherichia coli, Biotechnol. Bioeng. 42, 675-685.
22. Harlow, E. & Lane, D. (1988) Antibodies, a laboratory manual, Cold Spring Harbor Laboratory, Cold Spring Harbor NY.
23. Kay, W. W. & Gronlund, A. F. (1969) Amino acid transport in Pseudomonas aeruginosa, J. Bacteriol. 97, 273-281.
24. Kessler, S. W. (1975) Rapid isolation of antigens from cells with a staphylococcal protein A-antibody absorbent: parameters of the interaction of antibody-antigens complex with protein A, J. Immunol. 115, 1617-1624.
25. Kok, M., Oldenhuis, R., van der Linden, M. P. G., Meulenberg, C. H. C., Kingma, J. & Witholt, B. (1989a) The Pseudomonas oleovorans alkBAC operon encodes two structurally related rubredoxins and an aldehyde dehydrogenase, J. Biol. Chem. 264, 5442-5451.
26. Kok, M., Oldenhuis, R., van der Linden, M. P. G., Raatjes, P., van Lelyveld, P. H., Faber, K. N., Kingma, J. & Witholt, B. (1989b) The Pseudomonas oleovorans alkane hydroxylase gene: sequence and expression, J. Biol. Chem. 264, 5435-5441.
27. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680-685.
28. Lageveen, R. G. (1986) Oxidation of aliphatic compounds by Pseudomonas oleovorans. Biotechnological applications of the alkane-hydroxylase system, PhD thesis, University of Groningen, The Netherlands.
29. Mandelstam, J. (1958) Turnover of protein in growing and non-growing populations of Escherichia coli, Biochem. J. 169, 110-119.
30. McKenna, E. J. & Coon, M. J. (1970) Enzymatic ω-oxidation. IV. Purification and properties of the ω-hydroxylase of Pseudomonas oleovorans, J. Biol. Chem. 245, 3882-3889.
31. Miller, J. H. (1972) Experiments in molecular genetics, Cold Spring Harbor Laboratory Press, Cold Spring Harbor NY.
32. Mosteller, R. D., Goldstein, R. V. & Nishimoto, K. R. (1980) Metabolism of individual proteins in exponentially growing Escherichia coli, J. Biol. Chem. 255, 2524-2532.
33. Neidhardt, F. C. (1987) Chemical composition of Escherichia coli, in Escherichia coli and Salmonella typhimurium: cellular and molecular biology (Neidhardt, F. C., Ingraham, J. L., Low, K. B., Magasanik, B., Schaechter, M. & Umbarger, H. E., eds) vol. 1, pp. 3-6, American Society for Microbiology, Washington DC.
34. Nieboer, M., Kingma, J. & Witholt, B. (1993) The alkane oxidation system of Pseudomonas oleovorans, induction of the alk-genes in Escherichia coli W3110 affects membrane biogenesis and results in overexpression of alkane hydroxylase in a distinct cytoplasmic membrane subfraction, Mol. Microbiol. 8, 1039-1051.
35. Nieboer, M., Gunnewijk, M., van Beilen, J. B. & Witholt, B. (1997) Determinants for the overproduction of the Pseudomonas oleovorans cytoplasmic membrane protein alkane hydroxylase in alk+ E. coli W3110, J. Bacteriol., in the press.
36. Obukowicz, M. G., Straten, N. R. & Krivi, G. G. (1992) Enhanced heterologous gene expression in novel rpoH mutants of Escherichia coli, Appl. Environ. Microbiol. 58, 1511-1523.
37. Pai, S. R. & Kubitschek, H. E. (1992) Catabolic pools in Escherichia coli, Res. Microbiol. 143, 173-181.
38. Peterson, J. A., Basu, D. & Coon, M. J. (1966) Enzymatic ω-oxidation. I. Electron carriers in fatty acid and hydrocarbon hydroxylation, J. Biol. Chem. 241, 5162-5164.
39. Peterson, J. A., Kusunose, M., Kusunose, E. & Coon, M. J. (1967) Enzymatic ω-oxidation. II. Function of rubredoxin as the electron-carrier in ω-hydroxylation, J. Biol. Chem 242, 4334-4340.
40. Pine, M. J. (1970) Steady-state measurement of the turnover of amino acid in the cellular proteins of growing Escherichia coli: existence of two kinetically distinct reactions, J. Bacteriol. 103, 207-205.
41. Ramìrez, D. M. & Bentley, W. E. (1994) Fed-batch feeding and induction policies that improve foreign protein synthesis and stability by avoiding stress responses, Biotechnol. Bioeng. 47, 596-608.
42. Ruettinger, R. T., Olson, S. T., Boyer, R. F. & Coon, M. J. (1974) Identification of the ω-hydroxylase of Pseudomonas oleovorans as a non-heme iron protein requiring phospholipid for catalytic activity, Biochem. Biophys. Res. Commun. 57, 1011-1017.
43. Schwartz, R. D. & McCoy, C. J. (1973) Pseudomonas oleovorans hydroxylation-epoxidation system: Additional strain improvements, Appl. Microbiol. 26, 217-218.
44. Ueda, T. & Coon, M. J. (1972) Enzymatic ω-oxidation. VII. Reduced diphosphopyridine nucleotide-rubredoxin reductase: properties and function as an electron carrier in ω-hydroxylation. J. Biol. Chem. 247, 5010-5016.
45. van Beilen, J. B., Penninga, D. & Witholt, B. (1992) Topology of the membrane bound alkane hydroxylase of Pseudomonas oleovorans, J. Biol. Chem. 267, 9194-9201.
46. Vos-Scheperkeuter, G. H. & Witholt, B. (1984) Assembly pathway of newly synthesized LamB protein an outer membrane protein of Escherichia coli K-12, J. Mol. Biol. 175, 511-528.
47. Wensink, J. & Witholt, B. (1981a) Identification of different forms of the murein-bound lipoprotein found in isolated outer membranes of Escherichia coli, Eur. J. Biochem. 113, 349-357.
48. Wensink, J. & Witholt, B. (1981b) Conversion of free lipoprotein to the murein-bound form, Eur. J. Biochem. 117, 207-212.
49. Wijesundra, S. & Woods, D. D. (1960) Suppression of methionine biosynthesis in Escherichia coli by growth in the presence of this amino acid, J. Gen. Microbiol. 22, 229-241.
50. Witholt, B. (1972) Method for isolating mutants overproducing NAD and its precursors, J. Bacteriol. 109, 350-364.
51. Witholt, B., Boekhout, M., Brock, M., Kingma, J., van Heerikhuizen, H. & de Leij, L. (1976) An efficient and reproducible procedure for the formation of spheroplasts from variously grown Escherichia coli, Anal. Biochem. 74, 160-170.