Levels of tryptase, chymase, and FcεRIα messenger RNA in human skin are unchanged after IgE-dependent stimulation of cutaneous mast cells in vivo

Journal of Allergy and Clinical Immunology

Volumn 99, Issue 2, 1997, Pages 224-226

  Xia, Han Zhang ^a   Zweiman, Burton ^b   Schwartz, Lawrence B ^a,c  

^a VIRGINIA COMMONWEALTH UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

^c VIRGINIA COMMONWEALTH UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHYMASE; IMMUNOGLOBULIN E; MESSENGER RNA; TRYPTASE;

ALLERGY; ARTICLE; HUMAN; HUMAN CELL; INTERNALIZATION; LEUKOCYTE ACTIVATION; MAST CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; SECRETORY GRANULE;

ALLERGENS; BIOPSY; CHYMASES; FEMALE; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; HUMANS; MALE; MAST CELLS; POACEAE; POLYMERASE CHAIN REACTION; RECEPTORS, IGE; RESPIRATORY HYPERSENSITIVITY; RNA; RNA, COMPLEMENTARY; RNA, MESSENGER; SERINE ENDOPEPTIDASES; SKIN; TRYPTASES;

EID: 0031049011     PISSN: 00916749     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0091-6749(97)70100-3     Document Type: Article

References (5)

1. Dvorak AM, Schleimer RP, Lichtenstein LM. Human mast cells synthesize new granules during recovery from degranulation: in vitro studies with mast cells purified from human lungs. Blood 1988;71:76-85.
2. Vowels BR, Rook AH, Cassin M, Zweiman B. Expression of interleukin-4 and interleukin-5 mRNA in developing cutaneous late-phase reactions. J Allergy Clin Immunol 1995;96:92-6.
3. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987;162:156-9.
4. Xia H-Z, Kepley CL, Sakai K, Chelliah J, Irani A-MA, Schwartz LB. Quantitation of tryptase, chymase, FcεRIα, and FcεRIgamma mRNAs in human mast cells and basophils by competitive reverse transcription-polymerase chain reaction. J Immunol 1995;154:5472-80.
5. Atkins PC, Schwartz LB, Adkinson NF, von Airmen C, Valenzano M, Zweiman B. In vivo antigen-induced cutaneous mediator release: simultaneous comparisons of histamine, tryptase, and prostaglandin D2 release and the effect of oral corticosteroid administration. J Allergy Clin Immunol 1990;86:360-70.