Intestinal fatty acid binding protein: A specific residue in one turn appears to stabilize the native structure and be responsible for slow refolding

Protein Science

Volumn 6, Issue 2, 1997, Pages 364-372

  Kim, Keehyuk ^a   Ramanathan, Ragulan ^b   Frieden, Carl ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b WASHINGTON UNIVERSITY   (United States)

Author keywords

fatty acid binding; folding intermediate; hydrogen deuterium exchange

Indexed keywords

FATTY ACID BINDING PROTEIN;

ARTICLE; GENE MUTATION; MOLECULAR INTERACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FOLDING; PROTEIN STABILITY; STRUCTURE ANALYSIS;

EID: 0031048642     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560060212     Document Type: Article

References (19)

1. Banaszak L, Winter N, Xu Z, Bernlohr DA, Cowand S, Jones TA. 1994. Lipid-binding proteins: A family of fatty acid and retinoid transport proteins. Adv Protein Chem 45:89-151.
2. Beasty AM, Hurle MR, Manz JT, Stackhouse T, Onuffer JJ, Matthews CR. 1986. Effects of phenylalanine-22 → leucine, glutamic acid-49 → aspartic acid and glycine-234 → lysine mutations on the folding and stability of the α subunit of tryptophan synthase from Escherichia coli. Biochemistry 25:2965-2974.
3. Cistola DP, Kim K, Rogl H, Frieden C. 1996. Fatty acid interactions with a helix-less variant of intestinal fatty acid-binding protein. Biochemistry 35:7559-7565.
4. Frieden C, Jiang N, Cistola DP. 1995. Intestinal fatty acid binding protein: Folding of fluorescein-modified proteins. Biochemistry 34:2724-2730.
5. Gill SC, von Hippel PH. 1989. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem 182:319-326.
6. Gross ML. 1990. Tandem mass spectroscopy: Multisector magnetic instruments. Methods Enzymol 195:131-153.
7. Jiang N, Frieden C. 1993. Intestinal fatty acid binding protein: Characterization of mutant proteins containing inserted cysteine residues. Biochemistry 32:11015-11021.
8. Kim K, Cistola DP, Frieden C. 1996. Intestinal fatty acid-binding protein: The structure and stability of a helix-less variant. Biochemistry 35:7553-7558.
9. Kim PS, Baldwin RL. 1982. Specific intermediates in the folding reaction of small proteins and the mechanism of protein folding. Annu Rev Biochem 51:459-489.
10. Kraulis P. 1991. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structure. J Appl Crystallogr 24:946-950.
11. Ropson IJ, Frieden C. 1992. Dynamic NMR spectral analysis and protein folding: Identification of a highly populated folding intermediate of rat intestinal fatty acid-binding protein by 19F NMR. Proc Natl Acad Sci USA 89:7222-7226.
12. Ropson IJ, Gordon JI, Frieden C. 1990. Folding of a predominantly beta-structure protein: Rat intestinal fatty acid binding protein. Biochemistry 29:9591-9599.
13. Sacchettini JC, Gordon JI. 1993. Rat intestinal fatty acid binding protein. A model system for analyzing the forces that can bind fatty acids to proteins. [Review]. J Biol Chem 268:18399-18402.
14. Scapin G, Gordon JI, Sacchettini JC. 1992. Refinement of the structure of recombinant rat intestinal fatty acid-binding apoprotein at 1.2-Å resolution. J Biol Chem 267:4253-4269.
15. Strickland EH. 1974. Aromatic contributions to circular dichroism spectra of proteins. CRC Crit Rev Biochem 2:113-175.
16. Veerkamp JH, Maatman GHJ. 1995. Cytoplasmic fatty acid-binding proteins: Their structure and genes. Prog Lipid Res 34:17-52.
17. Xu ZH, Bernlohr DA, Banaszak LJ. 1993. The adipocyte lipid-binding protein at 1.6-Å resolution - Crystal structures of the apoprotein and with bound saturated and unsaturated fatty-acids. J Biol Chem 268:7874-7884.
18. Ybe JA, Hecht MH. 1996. Sequence replacements in the central beta-turn of plastocyanin. Protein Sci 5:814-824.
19. Zhang ZQ, Post CB, Smith DL. 1996. Amide hydrogen exchange determined by mass spectrometry - Application to rabbit muscle aldolase. Biochemistry 35:779-791.