Overexpression of cruzipain, the major cysteine proteinase of Trypanosoma cruzi, is associated with enhanced metacyclogenesis

European Journal of Biochemistry

Volumn 244, Issue 2, 1997, Pages 596-603

  Tomas, Ana M ^a   Miles, Michael A ^a   Kelly, John M ^a  

^a LONDON SCHOOL OF HYGIENE AND TROPICAL MEDICINE   (United Kingdom)

Author keywords

Cruzipain; Metacyclogenesis; Overexpression; Transfection; Trypanosoma cruzi

Indexed keywords

CRUZIPAIN;

ANIMAL CELL; ARTICLE; CELL CYCLE; CELL DIFFERENTIATION; CONTROLLED STUDY; COSMID; CYTOCHEMISTRY; ENZYME ACTIVITY; EPIMASTIGOTE; GENE OVEREXPRESSION; GENE TRANSFER; IMMUNOELECTRON MICROSCOPY; INFECTION; INSECT; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; TRYPANOSOMA CRUZI;

ANIMALIA; INSECTA; TRYPANOSOMA; TRYPANOSOMA CRUZI;

EID: 0031047603     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.t01-1-00596.x     Document Type: Article

References (35)

1. Aslund, L., Henriksson, J., Campetella, O., Frasch, A. C. C., Petterson, U. & Cazzulo, J. J. (1991) The C-terminal extension of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi, Mol. Biochem. Parasitol. 56, 103-116.
2. Berti, P. J. & Storer, A. C. (1995) Alignment/phylogeny of the papain superfamily of cysteine proteases, J. Mol. Biol. 246, 273-283.
3. Bonaldo, M. C., D'Escoffier, L. N., Salles, J. M. & Goldenberg, S. (1991a) Characterisation and expression of proteases during Trypanosoma cruzi metacyclogenesis, Exp. Parasitol. 73, 44-51.
4. Bonaldo, M. C., Scharfstein, J., Murta, A. C. M. & Goldenberg, S. (1991b) Further characterisation of Trypanosoma cruzi gp57/51 as the major antigen expressed by differentiating epimastigotes, Parasitol. Res. 77, 567-571.
5. Bontempi, E., Martinez, J. & Cazzulo, J. J. (1989) Subcellular localisation of a cysteine proteinase from Trypanosoma cruzi, Mol. Biochem. Parasitol. 33, 43-47.
6. Brener, Z. (1986) Why vaccines do not work in Chagas' disease, Parasitol. Today 2, 196-197.
7. Campetella, O., Henriksson, J., Aslund, L., Frasch, A. C. C., Pettersson, U. & Cazzulo, J. J. (1992) The major cysteine proteinase (cruzipain) of Trypanosoma cruzi is encoded by multiple polymorphic tandemly organised genes located on different chromosomes, Mol. Biochem. Parasitol. 50, 225-234.
8. Duboise, S. M., Vannier-Santos, M. A., Costo-Pinto, D., Rivas, L., Pan, A. A., Traub-Cseko, Y., De Souza, W. & McMahon-Pratt, D. (1994) The biosynthesis, processing, and immunolocalisation of Leishmania pifanoi amastigote cysteine proteinases, Mol. Biochem. Parasitol. 68, 119-132.
9. Eakin, A. E., Mills, A. A., Harth, G., McKerrow, J. H. & Craik, C. S. (1992) The sequence, organisation, and expression of the major cysteine protease (cruzain) from Trypanosoma cruzi, J. Biol. Chem. 267, 7411-7420.
10. Eakin, A. E., McGrath, M. E., McKerrow, J. H., Fletterick, R. J. & Craik, C. S. (1993) Production of crystallisable cruzain, the major cysteine protease from Trypanosoma cruzi, J. Biol. Chem. 268, 6115-6118.
11. Franke de Cazzulo, B. M., Martinez, J., North, M. J., Coombs, G. H. & Cazzulo, J. J. (1994) Effects of proteinase inhibitors on the growth and differentiation of Trypanosoma cruzi, FEMS Microbiol. Lett. 124, 81-86.
12. Greig, S. & Ashall, F. (1990) Electrophoretic detection of Trypanosoma cruzi peptidases, Mol. Biochem. Parasitol. 39, 31-38.
13. Gustafsson, L. L., Beerman, B. & Abdi, Y. A. (1987) Handbook of drugs for tropical parasitic diseases, Taylor and Francis Ltd, London.
14. Harth, G., Andrews, N., Mills, A. A., Engel, J. C., Smith, R. & McKerrow, J. H. (1993) Peptide-fluoromethyl ketones arrest replication and intracellular replication of Trypanosoma cruzi in vitro, Mol. Biochem. Parasitol. 58, 17-27.
15. Hoare, C. A. (1972) The trypanosomes of mammals, Blackwell Scientific Publications, Oxford, UK.
16. Kelly, J. M. (1993) Isolation of DNA and RNA from Leishmania, in Protocols in molecular parasitology (Hyde, J. E., ed.) pp. 123-131, Humana Press, Totowa NJ.
17. Kelly, J. M., Ward, H., Miles, M. A. & Kendall, G. (1992) A shuttle vector which facilitates the expression of transfected genes in Trypanosoma cruzi and Leishmania, Nucleic Acids Res. 20, 3963-3969.
18. Kelly, J. M., Das, P. & Tomas, A. M. (1994) An approach to functional complementation by introduction of large DNA fragments into Trypanosoma cruzi and Leishmania donovani using a cosmid shuttle vector, Mol. Biochem. Parasitol. 65, 51-62.
19. Kelly, J. M., Taylor, M. C., Rudenko, G. & Blundell, P. (1995) Transfection of the African and American trypanosomes, in Electroporation Protocols for Microorganisms (Nickoloff, J. A., ed.) pp. 349-359, Humana Press, Totowa NJ.
20. Kendall, G., Wilderspin, A. F., Ashall, F., Miles, M. A. & Kelly, J. M. (1990) Trypanosoma cruzi glycosomal glyceraldehyde 3-phosphate dehydrogenase does not conform to the 'hotspot' topogenic signal model, EMBO J. 9, 2751-2758.
21. Lima, A. P. C. de A., Tessier, D. C., Thomas, D. Y., Scharfstein, J., Storer, A. C. & Vernet, T. (1994) Identification of new cysteine protease isoforms in Trypanosoma cruzi, Mol. Biochem. Parasitol. 67, 333-338.
22. McKerrow, J. H., McGrath, M. E. & Engel, J. C. (1995) The cysteine protease of Trypanosoma cruzi as a model for antiparasitic drug design, Parasitol. Today 11, 279-282.
23. McGrath, M. E., Eakin, A. E., Engel, J. C., McKerrow, J. H., Craik, C. S. & Fletterick, R. J. (1995) The crystal structure of cruzain: a therapeutic target for Chagas' disease, J. Mol. Biol. 247, 251-259.
24. Meirelles, M. N. L., Juliano, L., Carmona, E., Silva, S. G., Costa, E. M., Murta, A. C. M. & Scharfstein, J. (1992) Inhibitors of the major cysteinyl proteinase (gp57/51) impair host cell invasion and arrest intracellular development of Trypanosoma cruzi in vitro, Mol. Biochem. Parasitol. 52, 175-184.
25. Miles, M. A. (1993) Culturing and biological cloning of Trypanosoma cruzi, in Protocols in molecular parasitology (Hyde, J. E., ed.) pp. 15-28, Humana Press, Totowa NJ.
26. Mottram, J. C., North, M. J., Barry, J. D. & Coombs, G. H. (1989) A cysteine proteinase cDNA from Trypanosoma brucei predicts an enzyme with an unusual C-terminal extension, FEBS Lett. 258, 211-215.
27. Mottram, J. C., Souza, A. E., Hutchinson, J. E., Carter, R., Frame, M. J. & Coombs, G. H. (1996) Evidence from the disruption of the lmcpb gene array of Leishmania mexicana that the cysteine proteinases are virulence factors, Proc. Natl Acad. Sci. USA 93, 6008-6013.
28. Murta, A. C. M., Persechini, P. M., Souto-Padron, T., De Souza, W., Guimaraes, J. A. & Scharfstein, J. (1990) Structural and functional identification of Gp57/51 antigen of Trypanosoma cruzi as a cysteine proteinase, Mol. Biochem. Parasitol. 43, 27-38.
29. Oullette, M. A. & Papadopoulou, B. (1993) Mechanisms of drug resistance in Leishmania, Parasitol. Today 9, 150-153.
30. Scharfstein, J., Schecter, M., Senna, M., Peralta, J. M., Mendonca-Previato, L. & Miles, M. A. (1986) Trypanosoma cruzi: characterisation and isolation of a 57/51,000 MW surface glycoprotein expressed by epimastigotes and bloodstream trypomastigotes, J. Immunol. 137, 1336-1341.
31. Soares, M. J., Souto-Padron, T. & De Souza, W. (1992) Identification of a large pre-lysosomal compartment in the pathogenic protozoan Trypanosoma cruzi, J. Cell Sci. 102, 157-167.
32. Souto-Padron, T., Campetella, O. E., Cazzulo, J. J. & De Souza, W. (1990) Cysteine proteinase in Trypanosoma cruzi: immunocytochemical localisation and involvement in parasite-host cell interaction, J. Cell Sci. 96, 485-490.
33. Souza, A. E., Waugh, S., Coombs, G. H. & Mottram, J. C. (1992) Characterisation of a multicopy gene for a major stage-specific proteinase of Leishmania mexicana, FEBS Lett. 311, 124-127.
34. Tomas, A. M. & Kelly, J. M. (1996) Stage-regulated expression of cruzipain the major cysteine proteinase of Trypanosoma cruzi is independent of the level of RNA, Mol. Biochem. Parasitol. 76, 91-103.
35. World Health Organisation (1990) Chagas' disease: frequency and geographical distribution, Weekly Epidemiol. Res. 65, 257-262