Insertion of foreign random sequences of 120 amino acid residues into an active enzyme

FEBS Letters

Volumn 402, Issue 2-3, 1997, Pages 177-180

  Doi, Nobuhide ^a,b   Itaya, Mitsuhiro ^b   Yomo, Tetsuya ^c   Tokura, Seiichi ^a   Yanagawa, Hiroshi ^b  

^a HOKKAIDO UNIVERSITY   (Japan)

^b MITSUBISHI KASEI INST OF LIFE SCI   (Japan)

^c OSAKA UNIVERSITY   (Japan)

Author keywords

Insertional mutagenesis; Protein evolution

Indexed keywords

AMINO ACID; MUTANT PROTEIN; RIBONUCLEASE H;

ARTICLE; ESCHERICHIA COLI; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE;

AMINO ACID SEQUENCE; CIRCULAR DICHROISM; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE LIBRARY; MODELS, STRUCTURAL; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL; MUTAGENESIS, SITE-DIRECTED; POLYMERASE CHAIN REACTION; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; RIBONUCLEASE H, CALF THYMUS;

ESCHERICHIA COLI;

EID: 0031047590     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-5793(96)01522-0     Document Type: Article

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