Investigation of ribosome binding by the shiga toxin A1 subunit, using competition and site-directed mutagenesis

Journal of Bacteriology

Volumn 179, Issue 4, 1997, Pages 1368-1374

  Skinner, L M ^a,b   Jackson, M P ^a  

^a WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b PENN STATE MILTON S HERSHEY MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RIBOSOME INACTIVATING PROTEIN; RIBOSOME RNA; SHIGA TOXIN;

ARTICLE; BINDING COMPETITION; ENZYME ACTIVITY; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; RIBOSOME; SITE DIRECTED MUTAGENESIS;

ESCHERICHIA COLI; EUKARYOTA;

EID: 0031046389     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.4.1368-1374.1997     Document Type: Article

References (34)

1. Boyer, H. W., and D. Roulland-Dussoix. 1969. A complementation analysis of the restriction and modification of DNA in Escherichia coli J. Mol. Biol. 41:459-472.
2. Deresiewicz, R. L., S. B. Calderwood, J. D. Robertus, and R. J. Collier. 1992. Mutations affecting the activity of Shiga-like toxin I A-chain. Biochemistry 31:3272-3280.
3. Endo, Y., Y.-L. Chan, A. Lin, K. Tsurugi, and I. G. Wool. 1988. The cytotoxins α-sarcin and ricin retain their specificity when tested on a synthetic oligoribonucleotide (35-mer) that mimics a region of 28S ribosomal ribonucleic acid. J. Biol. Chem. 263:7917-7920.
4. Endo, Y., A. Gluck, and I. G. Wool. 1991. Ribosomal RNA identity elements for ricin A-chain recognition and catalysis. J. Mol. Biol. 221:193-207.
5. Endo, Y., K. Mitsui, M. Motizuki, and K. Tsurugi. 1987. The mechanism of action of ricin and related toxic lectins on eucaryotic ribosomes. J. Biol. Chem. 262:5908-5912.
6. Endo, Y., and K. Tsurugi. 1987. RNA N-glycosidase activity of ricin A-chain. J. Biol. Chem. 262:8128-8130.
7. Endo, Y., K. Tsurugi, and J. M. Lambert. 1988. The site of action of six different ribosome-inactivating protein from plants on eukaryotic ribosomes: the RNA N-glycosidase activity of the proteins. Biochem. Biophys. Res. Commun. 150:1032-1036.
8. Endo, Y., K. Tsurugi, T. Yutsudo, Y. Takeda, T. Ogasawara, and K. Igarashi. 1988. Site of action of a Vero toxin (VT2) from Escherichia coli O157:H7 and of Shiga toxin on eucaryotic ribosomes. Eur. J. Biochem. 171:45-50.
9. Fernandez-Puentes, C., S. Benson, S. Olsnes, and A. Pihl. 1976. Protective effect of elongation factor 2 on the inactivation of ribosomes by the toxic lectins abrin and ricin. Eur. J. Biochem. 64:437-443.
10. Fraser, M. E., M. M. Chernaia, Y. V. Koslov, and M. N. G. James. 1994. Crystal structure of the holotoxin from Shigella dysenteriae at 2.5 Å resolution. Struct. Biol. 1:59-64.
11. Garred, O., B. van Deurs, and K. Sandvig. 1995. Furin-induced cleavage and activation of Shiga toxin. J. Biol. Chem. 270:10817-10821.
12. Gentry, M. K., and J. M. Dalrymple. 1980. Quantitative microtiter assay for Shigella toxin. J. Clin. Microbiol. 12:361-366.
13. Greener, A. 1990. E coli SURE strain: clone "unclonable" DNA. Strategies 3:5-6.
14. Guillot, D., F. Penin, A. DiPietro, B. Sontag, J. P. Lavergne, and J. P. Reboud. 1993. GTP binding to elongation factor eEf-2 unmasks a tryptophan residue required for biological activity. J. Biol. Chem. 268:20911-20916.
15. Haddad, J. E., and M. P. Jackson. 1993. Identification of the Shiga toxin A-subunit residues required for holotoxin assembly. J. Bacteriol. 175:7652-7657.
16. Hovde, C. J., S. B. Calderwood, J. J. Mekalanos, and R. J. Collier. 1988. Evidence that glutamic acid 167 is an active site residue of Shiga-like toxin I. Proc. Natl. Acad. Sci. USA 85:2568-2572.
17. Jackson, M. P. 1990. Structure-function analyses of Shiga toxin and the Shiga-like toxins. Microb. Pathog. 8:235-242.
18. Jackson, M. P., R. L. Deresiewicz, and S. B. Calderwood. 1990. Mutational analysis of the Shiga toxin and Shiga-like toxin II enzymatic subunits. J. Bacteriol. 172:3346-3350.
19. Jackson, M. P., E. A. Wadolkowski, D. L. Weinstein, R. K. Holmes, and A. D. O'Brien. 1989. Functional analysis of the Shiga toxin and Shiga-like toxin type II variant binding subunits by using site-directed mutagenesis. J. Bacteriol. 172:653-658.
20. Katzin, B. J., E. J. Collins, and J. D. Robertus. 1991. Structure of ricin A-chain at 2.5Å. Proteins 10:251-259.
21. Liu, Z., D. B. Batt, and G. G. Carmichael. 1994. An improved rapid method of isolating RNA from cultured cells by SDS-acid phenol/chloroform extraction. BioTechniques 16:56-57.
22. Lundblad, R. L. 1991. Chemical reagents for protein modification, 2nd ed. CRC Press, Inc., Boca Raton, Fla.
23. Maden, B. E. H. 1988. Locations of methyl groups in 28S rRNA of Xenopus laevis and man. J. Mol. Biol. 201:289-314.
24. Ready, M. P., Y. Kim, and J. D. Robertus. 1991. Site-directed mutagenesis of ricin A-chain and implications for the mechanism of action. Proteins 10:270-278.
25. Saiki, R. K., D. H. Gelfand, S. Stoffel, S. J. Scharf, R. Higuchi, G. T. Horn, K. B. Mullis, and H. A. Erlich. 1988. Primer-directed enzymatic amplification of DNA with thermostable DNA polymerase. Science 239:487-491.
26. Sallustio, S., and P. Stanley. 1990. Isolation of Chinese hamster ovary ribosomal mutants differentially resistant to ricin, .abrin, and modeccin. J. Biol. Chem. 265:582-588.
27. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
28. Sandvig, K., S. Olsnes, J. E. Brown, O. W. Peterson, and B. van Deurs. 1989. Endocytosis from coated pits of Shiga toxin: a glycolipid-binding protein from Shigella dysenteriae 1. J. Cell Biol. 108:1331-1343.
29. Schlossman, D., D. Withers, P. Welsh, A. Alexander, J. Robertus, and A. Frankel. 1989. Role of glutamic acid 177 of the ricin toxin A chain in enzymatic inactivation of ribosomes. Mol. Cell. Biol. 9:5012-5021.
30. Stirpe, F., S. Bailey, S. P. Miller, and J. W. Bodley. 1988. Modification of ribosomal RNA by ribosome-inactivating proteins from plants. Nucleic Acids Res. 16:1349-1357.
31. Studier, F. W., and B. A. Moffat. 1986. Use of bacteriophage T7 RNA polymerase to direct selective high level expression of cloned genes. J. Mol. Biol. 189:113-130.
32. Watanabe, K., E. Honjo, T. Tsukamoto, and G. Funatsu. 1992. Fluorescence studies on the interaction of adenine with ricin A-chain. FEBS Lett. 304:249-251.
33. Yamasaki, S., M. Furutani, K. Ito, K. Igarashi, M. Nishibuchi, and Y. Takeda. 1991. Importance of arginine at position 170 of the A subunit of verotoxin 1 produced by enterohemorrhagic E coli for toxin activity. Microb. Pathog. 11:1-9.
34. Zamboni, M., M. Brigotti, F. Rambelli, L. Montanaro, and S. Sperti. 1989. High-pressure-liquid-chromatographic and fluorimetric methods for the determination of adenine released from ribosomes by ricin and gelonin. Biochem. J. 259:639-643.