The cDNA cloning and transient expression of a chicken gene encoding cytochrome P-450scc

Gene

Volumn 185, Issue 2, 1997, Pages 217-222

  Nomura, Osamu ^a   Nakabayashi, Osamu ^a   Nishimori, Katsuhiko ^a   Mizuno, Shigeki ^a  

^a TOHOKU UNIVERSITY   (Japan)

Author keywords

Conserved sequence region; Enzyme assay; Splicing out of polyadenylation signal; Transfection

Indexed keywords

25 HYDROXYCHOLESTEROL; COMPLEMENTARY DNA; CYTOCHROME P450; MESSENGER RNA PRECURSOR; PREGNENOLONE;

ADRENAL GLAND; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CHICKEN; COMPARATIVE STUDY; CONTROLLED STUDY; ENZYME ACTIVITY; FEMALE; GENE EXPRESSION; GENETIC TRANSFECTION; MALE; MAMMAL; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYADENYLATION; PRIORITY JOURNAL; PROTEIN DOMAIN; RAINBOW TROUT; RNA SPLICING;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CHICKENS; CHOLESTEROL SIDE-CHAIN CLEAVAGE ENZYME; CLONING, MOLECULAR; COS CELLS; DNA, COMPLEMENTARY; FEMALE; MALE; MAMMALS; MOLECULAR SEQUENCE DATA; ONCORHYNCHUS MYKISS; OVARY; RECOMBINANT PROTEINS; RNA SPLICING; RNA, MESSENGER; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; TESTIS; TISSUE DISTRIBUTION; TRANSFECTION;

ANIMALIA; GALLUS GALLUS; MAMMALIA; ONCORHYNCHUS MYKISS; SALMONIDAE;

EID: 0031045149     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(96)00645-2     Document Type: Article

References (26)

1. Abraham, G.E., Buster, J.E., Kyle, F.W., Corrales, P.C. and Teller, R.C. (1973) Radioimmunoassay of plasma pregnenolone. J. Clin. Endocrinol. Metab. 37, 40-45.
2. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. and Struhl, K. (Eds.) (1987) Current Protocols in Molecular Biology, Vol. 1. Wiley, New York, pp. 9.1.1-9.1.9.
3. Bergman, T. and Postlind, H. (1991) Characterization of mitochondrial cytochrome P-450 from pig kidney and liver catalysing 26-hydroxylation of 25-hydroxyvitamin D3 and C27 steroids. Biochem. J. 276, 427-432.
4. Chung, B.-c., Matteson, K.J., Voutilainen, R., Mohandas, T.K. and Miller, W.L. (1986) Human cholesterol side-chain cleavage enzyme, P450scc: cDNA cloning, assignment of the gene to chromosome 15, and expression in the placenta. Proc. Natl. Acad. Sci. USA 83, 8962-8966.
5. Elbrecht, A. and Smith, R.G. (1992) Aromatase enzyme activity and sex determination in chickens. Science 255, 467-470.
6. Feinberg, A.P. and Vogelstein, B. (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132, 6-13.
7. Henikoff, S. (1984) Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28, 351-359.
8. Herbomel, P., Bourachot, B. and Yaniv, M. (1984) Two distinct enhancers with different cell specificities coexist in the regulatory region of polyoma. Cell 39, 653-662.
9. Hurt, E.C. and Van Loon, A.P.G.M. (1986) How proteins find mitochondria and intramitochondrial compartments. Trends Biochem. Sci. 11, 204-207.
10. Kimura, S., Hanioka, N., Matsunaga, E. and Gonzalez, F.J. (1989) The rat clofibrate-inducible CYP4A gene subfamily I. Complete intron and exon sequence of the CYP4A1 and CYP4A2 genes, unique exon organization, and identification of a conserved 19-bp upstream element. DNA 8, 503-516.
11. Lill, R. and Neupert, W. (1996) Mechanisms of protein import across the mitochondrial outer membrane. Trends Cell Biol. 6, 56-61.
12. Mathew, P.A., Kagawa, N., Bhasker, C.R. and Waterman, M.R. (1990) Deduced amino acid sequence of heme binding region of chicken cholesterol side chain cleavage cytochrome P450. Prot. Seq. Data Anal. 3, 323-325.
13. arom (aromatase) that catalyzes the formation of estrogen from androgen. J. Biol. Chem. 263, 16358-16363.
14. Minowa, O., Sogawa, K., Higashi, Y. and Fujii-Kuriyama, Y. (1990) Functional expression of microsomal and mitochondrial cytochrome P-450 (d and SCC) in COS-7 cells from cloned cDNA. Cell Struct. Funct. 15, 21-30.
15. Morohashi, K., Fujii-Kuriyama, Y., Okada, Y., Sogawa, K., Hirose, T., Inayama, S. and Omura, T. (1984) Molecular cloning and nucleotide sequence of cDNA for mRNA of mitochondrial cytochrome P-450(SCC) of bovine adrenal cortex. Proc. Natl. Acad. Sci. USA 81, 4647-4651.
16. Mulheron, G.W., Stone, R.T., Miller, W.L. and Wise, T. (1989) Nucleotide sequence of cytochrome P-450 cholesterol side-chain cleavage cDNA isolated from porcine testis. Nucleic Acids Res. 17, 1773.
17. 5→4 isomerase unique to major steroidogenic tissues. Gene 162, 261-265.
18. 3. J. Biol. Chem. 266, 8690-8695.
19. Ono, H., Iwasaki, M., Sakamoto, N. and Mizuno, S. (1988) cDNA cloning and sequence analysis of a chicken gene expressed during the gonadal development and homologous to mammalian cytochrome P-450c17. Gene 66, 77-85.
20. Oonk, R.B., Krasnow, J.S., Beattie, W.G. and Richards, J.S. (1989) Cyclic AMP-dependent and -independent regulation of cholesterol side chain cleavage cytochrome P-450 (P-450scc) in rat ovarian granulosa cells and corpora lutea. J. Biol. Chem. 264, 21934-21942.
21. Picado-Leonard, J. and Miller, W.L. (1988) Homologous sequences in steroidogenic enzymes, steroid receptors and a steroid binding protein suggest a consensus steroid- binding sequence. Mol. Endocrinol. 2, 1145-1150.
22. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual, 2nd ed., Vol. 1. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, p. 7.43.
23. 1 metabolism. Biochemistry 28, 7546-7554.
24. Takahashi, M., Tanaka, M., Sakai, N., Adachi, S., Miller, W.L. and Nagahama, Y. (1993) Rainbow trout ovarian cholesterol side-chain cleavage cytochrome P450 (P450scc); cDNA cloning and mRNA expression during oogenesis. FEBS Lett. 319, 45-48.
25. von Heijne, G. (1986) Mitochondrial targeting sequences may form amphiphilic helices. EMBO J. 5, 1335-1342.
26. Wang, Y. and Weiner, H. (1994) Evaluation of electrostatic and hydrophobic effects on the interaction of mitochondrial signal sequences with phospholipid bilayers. Biochemistry 33, 12860-12867.