Effects of hypertonia on voltage-gated ion currents in freshly isolated hippocampal neurons, and on synaptic currents in neurons in hippocampal slices

Brain Research

Volumn 748, Issue 1-2, 1997, Pages 157-167

  Huang, Rong ^a   Somjen, George G ^a  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

cell volume; excitatory postsynaptic current; freshly isolated cell; hypertonia; ion channel; osmotic effect; perforated patch clamp; synaptic current; voltage gated current; whole cell patch clamp

Indexed keywords

MANNITOL;

ANIMAL TISSUE; ARTICLE; BRAIN SLICE; CALCIUM CURRENT; CONTROLLED STUDY; ELECTROPHYSIOLOGY; HIPPOCAMPUS; MUSCLE HYPERTONIA; NONHUMAN; PATCH CLAMP; POTASSIUM CURRENT; PRIORITY JOURNAL; RAT; SODIUM CURRENT;

ANIMALS; CALCIUM CHANNELS; CELL SEPARATION; ELECTROPHYSIOLOGY; HIPPOCAMPUS; HYPERTONIC SOLUTIONS; ION CHANNEL GATING; ION CHANNELS; NEURONS; PATCH-CLAMP TECHNIQUES; POTASSIUM CHANNELS; RATS; RATS, SPRAGUE-DAWLEY; SYNAPSES;

EID: 0031045051     PISSN: 00068993     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-8993(96)01294-2     Document Type: Article

References (35)

1. Abe, Y., Furukawa, K., Itoyama, Y. and Akaike, N., Glycine response in acutely dissociated ventromedial hypothalamic neurons of the rat: new approach with gramicidine perforated patch technique, J. Neurophysiol., 72 (1994) 1530-1537.
2. Andrew, R.D., Seizure and acute osmotic change: clinical and neurophysiological aspects, J. Neurol. Sci., 101 (1991) 7-18.
3. Balestrino, M. and Somjen, G.G., Concentration of carbon dioxide, interstitial pH and synaptic transmission in hippocampal formation of the rat, J. Physiol. (Lond.), 396 (1988) 247-266.
4. Ballyk, B.A., Quackenbush, S.J. and Andrew, R.D., Osmotic effects on the CA1 neuronal population in hippocampal slices with special reference to glucose, J. Neurophysiol., 65 (1991) 1055-1066.
5. Blanton, M.G., Turco, J.J.L. and Kriegstein, A.R., Whole cell recording from neurons in slices of reptilian and mammalian cerebral cortex, J. Neurosci. Meth., 30 (1989) 203-210.
6. ++ currents by hypertonicity, Soc. Neurosci. Abst., 16 (1990) 505.
7. 2+-dependent stimulation of quantal neurosecretion in avian ciliary ganglion neurons, J. Neurophysiol., 68 (1992) 1229-1234.
8. Chebabo, S.R., Hester, M.A., Aitken, P.G. and Somjen, G.G., Hypotonie exposure enhances synaptic transmission and triggers spreading depression in hippocampal tissue slices, Brain Res., 695 (1995) 203-216.
9. 2+ influx, J. Neurophysiol., 73 (1995) 2553-2557.
10. Czéh, G., Aitken, P.G. and Somjen, G.G., Membrane currents in CA1 pyramidal cells during spreading depression (SD) and SD-like hypoxic depolarization, Brain Res., 632 (1993) 195-208.
11. Dudek, F.E., Obenaus, A. and Tasker, J.G., Osmolality-induced changes in extracellular volume alter epileptiform bursts independently of chemical synapses in the rat: importance of non-synaptic mechanisms in hippocampal epileptogenesis, Neurosci. Lett., 120 (1990) 267-270.
12. Feig, P.U. and McCurdy, D.K., The hypertonic state, New Engl. J. Med., 26 (1977) 1444-1453.
13. Fisher, R.E., Gray, R. and Johnston, D., Properties and distribution of single voltage-gated calcium channels in adult hippocampal neurons, J. Neurophysiol., 64 (1990) 91-104.
14. Hamill, O.P., Marty, A., Neher, E., Sakmann, B. and Sigworth, F.J., Improved patch-clamp technique for high resolution current recording from cells and cell-free patches, Pflügers Arch., 391 (1981) 85-100.
15. Hille, B., Ionic Channels of Excitable Membranes, Edn. 2, Sinauer Associates, Sunderland, MA, 1992.
16. Huang, R., Aitken, P.G. and Somjen, G.G., The extent and mechanism of the loss of function caused by strongly hypotonie solutions in rat hippocampal tissue slices, Brain Res., 695 (1995) 195-202.
17. Huang, R., Aitken, P.G. and Somjen, G.G., Hypertonic environment attenuates depolarization and improves recovery from hypoxia in hippocampal tissue slices, J. Cereb. Blood Flow Metab., 16 (1996) 462-467.
18. + currents of freshly isolated hippocampal neurons during graded and prolonged hypertonic exposure, Pflügers Arch., 430, Suppl. (1995) R122 (abstract).
19. Huang, R. and Somjen, G.G., The effect of graded hypertonia on interstitial volume, tissue resistance and synaptic transmission in rat hippocampal tissue slices, Brain Res., 702 (1995) 181-187.
20. Hubbard, J.I., Jones, S.F. and Landau, E.M., An examination of the effects of osmotic pressure changes upon transmitter release from mammalian motor nerve terminals, J. Physiol. (Lond.), 197 (1968) 639-657.
21. Karst, H., Joëls, M. and Wadman, W.J., Low-threshold calcium current in dendrites of the adult rat hippocampus, Neurosci. Lett., 164 (1993) 154-158.
22. Kay, A.R. and Wong, R.K.S., Isolation of neurons suitable for patch clamping from adult mammalian central nervous system, J. Neurosci. Meth., 16 (1986) 227-238.
23. Kyrozis, A. and Reichling, D.B., Perforated-patch recording with gramicidin avoids artifactual changes in intracellular chloride concentration, J. Neurosci. Meth., 57 (1995) 27-35.
24. Loeb, J.L., The hyperosmolar state, New Engl. J. Med., 290 (1974) 1184-1187.
25. Numann, R.E., Wadman, W.J. and Wong, R.K.S., Outward currents of single hippocampal cells obtained from the adult guinea pig, J. Physiol. (Lond.), 393 (1987) 331-353.
26. Paoletti, P. and Ascher, P., Mechanosensitivity of NMDA receptors in cultured mouse central neurons, Neuron, 13 (1994) 645-655.
27. Rhee, J.-S., Ebihara, S. and Akaike, N., Gramicidin perforated patch-clamp technique reveals glycine-gated outward chloride current in dissociated nucleus solitarii neurons of the rat, J. Neurophysiol, 72(1994) 1103-1108.
28. Somjen, G.G., Faas, G.C., Vreugdenhil, M. and Wadman, W.J., Channel shutdown: a response of hippocampal neurons to adverse environments, Brain Res., 632 (1993) 180-194.
29. Somjen, G.G. and Tombaugh, G.C., pH modulation of neuronal excitability and central nervous system functions. In: K. Kaila and B.R. Ransom (Eds.), pH and Brain Function, Wiley, New York, 1996, in press.
30. Somjen, G.G., Wadman, W.J., Juta, A., Borgdorf, A., Aitken, P.G. and Kiehart, D.P., Osmotically induced volume changes of freshly isolated hippocampal neurons, FASEB J., 10 (1996) A86 (abstract).
31. Takahashi, K., Wakamori, M. and Akaike, N., Hippocampal CA1 pyramidal cells of rats have four voltage-dependent calcium conductances, Neurosci. Lett., 104 (1989) 229-234.
32. Tombaugh, G.C., Mild acidosis delays hypoxic spreading depression and improves neuronal recovery in rat hippocampal slices, J. Neurosci., 14 (1994) 5635-5643.
33. 2+ currents in isolated rat CA1 neurons, J. Physiol. (Lond.), 493 (1996) 719-732.
34. + sensitivity of excitatory amino acid receptor channels in rat cerebellar granule cells, J. Physiol. (Lond.), 433 (1991) 727-763.
35. Vreugdenhil, M., Somjen, G.G. and Wadman, W.J., Effects of strongly anisosmotic and NaCl deficient solutions on muscimol- and glutamate-evoked whole-cell currents in freshly dissociated hippocampal neurons, Brain Res., 670 (1995) 89-96.