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Volumn 22, Issue 2, 1997, Pages 312-318

Highly sensitive method to detect mRNAs in individual cells by direct RT-PCR using Tth DNA polymerase

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE;

EID: 0031044819     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97222st04     Document Type: Article
Times cited : (19)

References (8)
  • 1
    • 0028966584 scopus 로고
    • Functional isolation and characterization of human hematopoietic stem cells
    • Berardi, A.C., A. Wang, J.D. Levine, P. Lopez and D.T. Scadden. 1995. Functional isolation and characterization of human hematopoietic stem cells. Science 267:104-108.
    • (1995) Science , vol.267 , pp. 104-108
    • Berardi, A.C.1    Wang, A.2    Levine, J.D.3    Lopez, P.4    Scadden, D.T.5
  • 2
    • 0028174829 scopus 로고
    • Detection of mRNA expression in a single cell by direct RT-PCR
    • Kumazaki, T., K. Hamada and Y. Mitsui. 1994. Detection of mRNA expression in a single cell by direct RT-PCR. BioTechniques 16:1017-1019.
    • (1994) BioTechniques , vol.16 , pp. 1017-1019
    • Kumazaki, T.1    Hamada, K.2    Mitsui, Y.3
  • 3
    • 0026523109 scopus 로고
    • Co-engagement of CD3 with LFA-1 or ICAM-1 adhesion molecules enhances the frequency of activation of single murine CD4+ and CD8+ T cells and induces synthesis of IL-3 and IFN-gamma but not IL-4 or IL-6
    • Maraskovsky, E., A.B. Troutt and A. Kelso. 1992. Co-engagement of CD3 with LFA-1 or ICAM-1 adhesion molecules enhances the frequency of activation of single murine CD4+ and CD8+ T cells and induces synthesis of IL-3 and IFN-gamma but not IL-4 or IL-6. Int. Immunol. 4:475-485.
    • (1992) Int. Immunol. , vol.4 , pp. 475-485
    • Maraskovsky, E.1    Troutt, A.B.2    Kelso, A.3
  • 4
    • 0028026306 scopus 로고
    • RT-PCR assay for detection of transcripts from very few cells using whole cell lysates
    • O'Brien, D.P., D. Billadeau and B. Van Ness. 1994. RT-PCR assay for detection of transcripts from very few cells using whole cell lysates. BioTechniques 16:586-590.
    • (1994) BioTechniques , vol.16 , pp. 586-590
    • O'Brien, D.P.1    Billadeau, D.2    Van Ness, B.3
  • 5
    • 0024571529 scopus 로고
    • Novel method for studying mRNA phenotypes in single or small numbers of cells
    • Rappolee, D.A., A. Wang, D. Mark and Z. Werb. 1989. Novel method for studying mRNA phenotypes in single or small numbers of cells. J. Cell. Biochem. 39:1-11.
    • (1989) J. Cell. Biochem. , vol.39 , pp. 1-11
    • Rappolee, D.A.1    Wang, A.2    Mark, D.3    Werb, Z.4
  • 6
    • 0025959560 scopus 로고
    • Follicular dendritic cells contain a unique gene repertoire demonstrated by single-cell polymerase chain reaction
    • Schriever, F., G. Freeman and L.M. Nadler. 1991. Follicular dendritic cells contain a unique gene repertoire demonstrated by single-cell polymerase chain reaction. Blood 77:787-791.
    • (1991) Blood , vol.77 , pp. 787-791
    • Schriever, F.1    Freeman, G.2    Nadler, L.M.3
  • 8
    • 0026487207 scopus 로고
    • Single-cell cDNA-PCR: Removal of contaminating genomic DNA from total RNA using immobilized DNase I
    • Ziegler, B.L., C. Lamping, S. Thoma and C.A. Thomas. 1992. Single-cell cDNA-PCR: removal of contaminating genomic DNA from total RNA using immobilized DNase I. BioTechniques 13:726-729.
    • (1992) BioTechniques , vol.13 , pp. 726-729
    • Ziegler, B.L.1    Lamping, C.2    Thoma, S.3    Thomas, C.A.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.