Real-time assay of the interaction of a GST fusion protein with a protein ligate using resonant mirror technique

BioTechniques

Volumn 22, Issue 2, 1997, Pages 269-271

  Rubio, Ignacio ^a   Buckle, Philip ^b   Trutnau, Hans ^c   Wetzker, Reinhard ^a  

^a FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

^b Affinity Sensors   (United Kingdom)

^c Affinity Sensors   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

HYBRID PROTEIN; PHOSPHATIDYLINOSITIDE;

ARTICLE; BINDING AFFINITY; IMMUNOASSAY; LIGAND BINDING; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN DETERMINATION;

EID: 0031044818     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97222bm15     Document Type: Article

References (6)

1. Buckle, P., R.J. Davis, T. Kinning, D. Yeung, P.R. Edwards, D. Pollard-Knight and C.R. Lowe. 1993. The resonant mirror: a novel optical biosensor for direct sensing of biomolecular interactions part II: applications. Biosens. Bioelectron. 8:355-363.
2. Cush, R., J.M. Cronin, W.J. Stewart, C.H. Maule, J. Molloy and N.J. Goddard. 1993. The resonant mirror: a novel optical biosensor for direct sensing of biomolecular interactions part I: principle of operation and associated instrumentation. Biosens. Bioelectron. 8:347-353.
3. End, P., I. Gout, M.J. Fry, G. Panayotou, R. Dhand, K. Yonezawa, M. Kasuga and M.D. Waterfield. 1993. A biosensor approach to probe the structure and function of the p85a subunit of the phosphatidylinositol 3-kinase complex. J. Biol. Chem. 268:10066-10075.
4. Liedberg, B., C. Nylander and I. Lundstrum. 1983. Surface plasmon resonance for gas detection and biosensing. Sens. Actuators 4:299-304.
5. Rodriguez-Viciana, P., P.H. Warne, R. Dhand, B. Vanhaesebroeck, I. Gout, M.J. Fry, M.D. Waterfield and J. Downward. 1994. Phosphatidylinositol-3-OH kinase as a direct target of Ras. Nature 370:527-532.
6. Stoyanov, B., S. Volinia, T. Hanck, I. Rubio, M. Loubchenkov, D. Malek, S. Stoyanova, B. Vanhaesebroeck et al. 1995. Cloning and characterization of a human G protein-activated phosphoinositide 3-kinase. Science 692:690-693.