Family of plasmid vectors for the expression of β-galactosidase fusion proteins in eukaryotic cells

BioTechniques

Volumn 22, Issue 2, 1997, Pages 254-258

  Ivics, Zoltán ^a   Izsvák, Zsuzsanna ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE;

ARTICLE; DNA DETERMINATION; ENZYME ACTIVITY; EUKARYOTE; GENE EXPRESSION; OPEN READING FRAME; PLASMID; PROTEIN ANALYSIS; SIGNAL TRANSDUCTION; SPECTROPHOTOMETRY;

EUKARYOTA;

EID: 0031043893     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97222bm12     Document Type: Article

References (11)

1. Beale, E.G., E-A. Deeb, R.S. Handley, H. Akhavan-Tafti and A.P. Schaap. 1992. A rapid and simple chemiluminescent assay for Escherichia coli β-galactosidase. BioTechniques 12:320-324.
2. Bétancourt, O.H., J. Attal, M.C. Théron, C. Puissant and L.M. Houdebine. 1993. Efficiency of introns from various origins in fish cells. Mol. Mar. Biol. Biotech. 2:181-188.
3. Edlund, T., M.D. Walker, P.J. Barr and W.J. Rutter. 1985. Cell-specific expression of the rat insulin gene: evidence for role of two distinct 5′ flanking elements. Science 230:912-916.
4. Eustice, D.C., P.A. Feldman, A.M. Colberg-Poley, R.M. Buckery and R.H. Neubauer. 1991. A sensitive method for the detection of β-galactosidase in transfected mammalian cells. BioTechniques 11:739-743.
5. Ivics, Z., Z. Izsvák, A. Minter and P.B. Hackett. 1996. Identification of functional domains and evolution of Tcl-like transposable elements. Proc. Natl. Acad. Sci. USA 93:5008-5013.
6. Kozak, M. 1987. An analysis of 5′-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res. 15:8125-8131.
7. Lin, S., S. Yang and N. Hopkins. 1994. LacZ expression in germline transgenic zebrafish can be detected in living embryos. Dev. Biol. 167:77-83.
8. MacGregor, G.R. and C.T. Caskey. 1989. Construction of plasmids that express E. coli β-galactosidase in mammalian cells. Nucleic Acids Res. 17:2365.
9. Minton, N.P. 1984. Improved plasmid vectors for the isolation of translational lac gene fusions. Gene 31:269-273.
10. Sanes, J.R., J.L.R. Rubinstein and J-F. Nicholas. 1986. Use of a recombinant retrovirus to study post-implantation cell lineage in mouse embryos. EMBO J. 5:3133-3142.
11. Zhang, Y-Z., J.J. Naleway, K.D. Larison, Z. Huang and R.P. Haugland. 1991. Detecting lacZ gene expression in living cells with new lipophilic, fluorogenic β-galactosidase substrates. FASEB J. 5:3108-3113.