Competitive PCR quantification of pro- and anti-inflammatory cytokine mRNA in the central nervous system during autoimmune encephalomyelitis

Journal of Neuroimmunology

Volumn 73, Issue 1-2, 1997, Pages 197-206

  Tanuma, Naoyuki ^a   Kojima, Takashi ^a   Shin, Taekyun ^a   Aikawa, Yukihiko ^a   Kohji, Toshihiko ^a   Ishihara, Yoshihiro ^a   Matsumoto, Yoh ^a  

^a TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE   (Japan)

Author keywords

competitive PCR; cytokine; experimental autoimmune encephalomyelitis

Indexed keywords

GAMMA INTERFERON; INTERLEUKIN 10; MESSENGER RNA; TRANSFORMING GROWTH FACTOR BETA1; TUMOR NECROSIS FACTOR ALPHA;

ALLERGIC ENCEPHALOMYELITIS; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; AUTOIMMUNITY; CONTROLLED STUDY; ETIOLOGY; FEMALE; MALE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RAT;

ANIMALS; CELL SEPARATION; CENTRAL NERVOUS SYSTEM; CYTOKINES; ENCEPHALOMYELITIS, AUTOIMMUNE, EXPERIMENTAL; FEMALE; FLOW CYTOMETRY; IMMUNOHISTOCHEMISTRY; INTERFERON TYPE II; MALE; POLYMERASE CHAIN REACTION; RATS; RATS, INBRED LEW; RNA, MESSENGER; TUMOR NECROSIS FACTOR-ALPHA;

EID: 0031043631     PISSN: 01655728     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-5728(96)00199-3     Document Type: Article

References (33)

1. Adams, D.O. and Hamilton, T.A. (1987) Molecular transductional mechanisms by which IFN-γ and other signals regulate macrophage development. Immunol. Rev. 97, 5-27.
2. Becker-André, M. and Hahlbrock, K. (1989) Absolute mRNA quantification using the polymerase chain reaction (PCR). A novel approach by a PCR aided transcript titration assay (PATTY). Nucleic Acids Res. 17, 9437-9446.
3. Gilliland, G., Perrin, S., Blanchard, K. and Bunn, H.F. (1990) Analysis of cytokine mRNA and DNA: Detection and quantitation by competitive polymerase chain reaction. Proc. Natl. Acad. Sci. USA 87, 2725-2729.
4. Hünig, T., Wallny, H., Hartley, J.K., Lawetzky, A. and Tiefenthaler, G. (1989) A monoclonal antibody to a constant determinant of the rat T cell receptor that induces T cell activation. Differential activity with subsets of immature and mature T lymphocytes. J. Exp. Med. 169, 73-86.
5. Issazadeh, S., Mustafa, M., Ljungdahl, Å., Höjeberg, B., Dagerlind, Å., Elde, R. and Ollson, T. (1995a) Interferon γ, interleukin 4 and transforming growth factor β in experimental autoimmune encephalomyelitis in Lewis rats: dynamics of cellular mRNA expression in the central nervous system and lymphoid cells. J. Neurosci. Res. 40, 579-590.
6. Issazadeh, S., Ljungdahl, Å., Höjeberg, B., Mustafa, M. and Ollson, T. (1995b) Cytokine production in the central nervous system of Lewis rats with experimental autoimmune encephalomyelitis: dynamics of mRNA expression for interleukin-10, interleukin-12, cytolysin, tumor necrosis factor α and tumor necrosis factor β. J. Neuroimmunol. 61, 205-212.
7. Johns, L.D., Flanders, K.C., Ranges, G.E. and Sriram, S. (1991) Successful treatment of experimental allergic encephalomyelitis with transforming growth factor-β1. J. Immunol. 147, 1792-1796.
8. + suppressor cells inhibit the function of effector cells of experimental autoimmune encephalomyelitis through a mechanism involving transforming growth factor-β. J. Immunol. 146, 1163-1168.
9. + suppressor cells of autoimmune encephalomyelitis respond to T cell receptor-associated determinants on effector cells by interleukin-4 secretion. Eur. J. Immunol. 22, 1757-1763.
10. Kennedy, M.K., Torrance, D.S., Picha, K.S. and Mohler, K.M. (1992) Analysis of cytokine mRNA expression in the central nervous system of mice with experimental autoimmune encephalomyelitis reveals that IL-10 mRNA expression correlates with recovery. J. Immunol. 149, 2496-2505.
11. Kiefer, R., Gold, R., Gehrmann, J., Lindholm, D., Wekerle, H. and Kreutzberg, G.W. (1993) Transforming growth factor beta expression in reactive spinal cord microglia and meningeal inflammatory cells during experimental allergic neuritis. J. Neurosci. Res. 36, 391-398.
12. Kiefer, R., Funa, K., Schweitzer, T., Jung, S., Bourde, O., Toyka, K.V. and Hartung, H-P. (1996) Transforming growth factor-β1 in experimental autoimmune neuritis. Cellular localization and time course. Am. J. Pathol. 148, 211-223.
13. Kuruvilla, A.P., Shah, R., Hochwald, G.M., Liggitt, H.D., Palladino, M.A. and Thorbecke, G.J. (1991) Protective effect of transforming growth factor β1 on experimental autoimmune disease in mice. Proc. Natl. Acad. Sci. USA 88, 2918-2921.
14. Matsumoto, Y. and Fijiwara, M. (1987) The immunopathology of adoptively transferred experimental allergic encephalomyelitis (EAE) in Lewis rats. Part 1. Immunohistochemical examination of developing lesion of EAE. J. Neurol. Sci. 77, 35-47.
15. Matsumoto, Y., Ohmori, K. and Fujiwara, M. (1992) Immune regulation by brain cells in the central nervous system: microglia but not astrocytes present myelin basic protein to encephalitogenic T cells under in vivo-mimicking conditions. Immunology 76, 209-216.
16. McDonald, A.H. and Swanborg, R.H. (1988) Antigen-specific inhibition of immune interferon production by suppressor cells of autoimmune encephalomyelitis. J. Immunol. 140, 1132-1138.
17. Mizuno, T., Sawada, M., Marunouchi, T. and Suzumura, A. (1994) Production of interleukin-10 by mouse glial cells in culture. Biochem. Biophys. Res. Commun. 205, 1907-1915.
18. Mustafa, M., Diener, P., Höjeberg, B., van der Meide, P. and Olsson, T. (1991) T cell immunity and interferon γ secretion during experimental allergic encephalomyelitis in Lewis rats. J. Neuroimmunol. 31, 165-177.
19. Ohmori, H., Hong, Y., Fujiwara, M. and Matsumoto, Y. (1992) In situ demonstration of proliferating cells in the rat central nervous system during experimental autoimmune encephalomyelitis. Evidence suggesting that most infiltrating T cells do not proliferate in the target organ. Lab. Invest. 66, 54-62.
20. Okuda, Y., Nakatsuji, Y., Fujimura, H., Esumi, H., Ogura, T., Yanagihara, T. and Sakoda, S. (1995) Expression of inducible isoform of nitric oxide synthase in the central nervous system of mice correlates with the severity of actively induced experimental allergic encephalomyelitis. J. Neuroimmunol. 62,103-112.
21. Panitch, H.S., Hirsh, R.L., Schindler, J. and Johnson, K.P. (1987) Treatment of multiple sclerosis with gamma interferon: exacerbations associated with activation of the immune system. Neurology 37, 1097-1102.
22. Racke, M.K., Dhib-Jalbut, S., Cannella, B., Albert, P.S., Raine, C.S. and McFarlin, D.E (1991) Prevention and treatment of chronic relapsing experimental allergic encephalomyelitis by transforming growth factor-β1. J. Immunol. 146, 3012-3017.
23. Raine, C.S. (1976) Experimental allergic encephalomyelitis and related conditions. In: H.M. Zimmerman (Ed.), Progress in Neuropathology, Vol. 3, Grune and Stratton, San Francisco, CA, pp. 225-251.
24. Renno, T., Krakowski, M., Piccirillo, C., Lin, J. and Owens, T. (1995) TNF-α expression by resident microglia and infiltrating leukocytes in the central nervous system of mice with experimental allergic encephalomyelitis. J. Immunol. 154, 944-953.
25. Rieckmann, P., Albrecht, M., Kitze, B., Weber, T., Tumani, H., Broocks, A., Lüer, W., Helwig, A. and Poser, S. (1995) Tumor necrosis factor-alpha messenger RNA expression in patients with relapsing-remitting multiple sclerosis is associated with disease activity. Ann. Neurol. 37, 82-88.
26. Robinson, A.P., White, T.M. and Mason, D.W. (1986) Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and OX-42, the latter recognizing complement receptor type 3. Immunology 57, 239-247.
27. Rott, O., Fleischer, B. and Cash, E. (1994) Interleukin-10 prevents experimental allergic encephalomyelitis in rats. Eur. J. Immunol. 24, 1434-1440.
28. Ruddle, N.H, Bergman, C.M., McGrath, KM, Lingenheld, E.G., Grunnet, M.L., Padula, S.J. and Clark, R.B. (1990) An antibody to lymphotoxin and tumor necrosis factor prevents transfer of experimental allergic encephalomyelitis. J. Exp. Med. 172, 1193-1200.
29. Schmidt, E.E. and Merrill, G.F. (1991) Changes in dihydrofolate reductase (DHFR) mRNA levels can account fully for changes in DHFR synthesis rates during terminal differentiation in a highly amplified myogenic cell line. Mol. Cell. Biol. 11, 3726-3734.
30. Selmaj, K., Raine, C.S. and Cross, A.H. (1991) Anti-tumor necrosis factor therapy abrogates autoimmune demyelination. Ann. Neurol. 30, 694-700.
31. Selmaj, K., Papierz, W., Glabinski, A. and Kohno, T. (1995) Prevention of chronic relapsing experimental autoimmune encephalomyelitis by soluble tumor necrosis factor receptor I. J. Neuroimmunol. 56, 135-141.
32. Siebert, P.D. and Larrick, J.W. (1992) Competitive PCR. Nature 359, 557-558.
33. Weinberg, A.D., Wallin, J.J., Jones, R.E., Sullivan, T.J., Bourdette, D.N., Vandenbark, A.A. and Offner, H. (1994) Target organ-specific up-regulation of the MRC OX-40 marker and selective production of Th1 lymphokine mRNA by encephalitogenic T helper cells isolated from the spinal cord of rats with experimental autoimmune encephalomyelitis. J. Immunol. 152, 4712-4721.