Monoclonal antibody detection of Pseudomonas spp. in refrigerated meat by an indirect ELISA

Letters in Applied Microbiology

Volumn 24, Issue 1, 1997, Pages 5-8

  Gutierrez, R ^a   Garcia T ^a   Gonzalez I ^a   Sanz, B ^a   Hernandez P E ^a   Martin R ^a  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; CHEMICAL DETECTION; SURFACE PLASMON RESONANCE;

ANTIBODY DETECTION; DETECTION THRESHOLD; ELISA ASSAY; LIVE CELL; PSEUDOMONAS FLUORESCENS; PSEUDOMONAS SPP;

MONOCLONAL ANTIBODIES;

BACTERIUM ANTIBODY;

ANIMAL TISSUE; ARTICLE; BACTERIUM IDENTIFICATION; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FOOD CONTAMINATION; MEAT; NONHUMAN; PSEUDOMONAS; SWINE; TECHNIQUE;

ANIMALIA; BACTERIA (MICROORGANISMS); PSEUDOMONAS; PSEUDOMONAS FLUORESCENS; SUS SCROFA;

EID: 0031043467     PISSN: 02668254     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1472-765X.1997.00329.x     Document Type: Article

References (16)

1. Appl, C.P. and Marshall, R.T. (1984) Detachment of Pseudomonas fluorescens P26 from beef rinsed in salt and acid solutions. Journal of Food Protection 47, 537-541.
2. Bishop, J.R. and White, C.H. (1986) Assessment of dairy product quality and potential shelf-life: a review. Journal of Food Protection 49, 739-753.
3. Candlish, A.A.G. (1991) Immunological methods in food microbiology. Food Microbiology 8, 1-14.
4. Eriksson, P.V., Graciela, N.P., Paseti, M.F. and Manghi, M.A. (1995) Inhibition enzyme-linked immunosorbent assay for detection of Pseudomonas fluorescens on meat surface. Applied and Environmental Microbiology 61, 397-398.
5. González, I., Martín, R., García, T., Morales, P., Sanz, B. and Hernández, P.E. (1994) A sandwich enzyme-linked immunosorbent assay (ELISA) for detection of Pseudomonas fluorescens and related psychrotrophic bacteria in refrigerated meat. Journal of Food Protection 57, 710-714.
6. González, I., Martín, R., García, T., Morales, P., Sanz, B. and Hernández, P.E. (1996) Polyclonal antibodies against the protein F of Pseudomonas fluorescens for the detection of psychrotrophic bacteria in meat using an indirect enzyme-linked immunosorbent assay. Meat Science 42, 305-313.
7. Harlow, E. and Lane, D. (ed.) (1988) Ammonium sulphate precipitation. In Antibodies. A Laboratory Manual. p. 258. Cold Spring Harbor, New York : Cold Spring Harbor Laboratory.
8. Köhler, G. and Milstein, C. (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256, 495-497.
9. Labadie, J. and Desnier, I. (1992) Selection of cell wall antigens for the rapid detection of bacteria by immunological methods. Journal of Applied Bacteriology 72, 220-226.
10. May, S.A., Mikolajcik, E.M. and Richter, E.R. (1989) Conventional tube and microplate Limulus amoebocyte lysate procedures for determination of Gram-negative bacteria in milk. Journal of Dairy Science 72, 1137-1141.
11. McMeekin, T.A. (1982) Microbial spoilage of meats. In Developments in Food Microbiology ed. Davies, R. pp. 1-40. London : Applied Science Publishers.
12. Pérez de Castro, B., Asensio, M.A., Sanz, B. and Ordóñez, J.A. (1988) A method to assess the bacterial content of refrigerated meat. Applied and Environmental Microbiology 54, 1462-1465.
13. Pettipher, G.L., Mansell, R., McKinnon, C.H. and Cousins, C.M. (1980) Rapid membrane filtration-epifluorescent microscopy technique for direct enumeration of bacteria in raw milk. Applied and Environmental Microbiology 39, 423-429.
14. Samarajeewa, U., Wei, C.I., Huang, T.S. and Marshall, M.R. (1991) Application of immunoassay in the food industry. Critical Reviews in Food Science and Nutrition 29, 403-434.
15. Sogaard, H. and Lund, R. (1981) A comparison of three methods for enumeration of psychrotrophic bacteria in raw milk. In Psychrotrophic Microorganisms in Spoilage and Pathogenicity ed. Roberts, T.A., Hobbs, G., Christian, J.H.B. and Skovgaard N. pp. 109-116. London: Academic Press.
16. Sharpe, A.N. (1994) Developments in rapid methods for detection of agents of foodborne disease. Food Research International 27, 237-243.