Flow cytometric applications of sulforhodamine 101 as a fluorescent stain for total cellular protein

Biotechnic and Histochemistry

Volumn 72, Issue 1, 1997, Pages 1-9

  Engelhard, Herbert H ^a,b  

^a NORTHWESTERN UNIVERSITY   (United States)

^b NONE   (United States)

Author keywords

4',6 diamidino 2 phenylindole; cell protein; flow cytometry; fluorescein isothiocyanate; fluorescence; gliobla stoma multiforme; multiparameter analysis; promyelocytic leukemia; sulforhodamine 101

Indexed keywords

CELL PROTEIN; FLUORESCEIN ISOTHIOCYANATE; FLUORESCENT DYE; RHODAMINE;

ANALYTIC METHOD; ARTICLE; BLAST TRANSFORMATION; CELL SIZE; FLOW CYTOMETRY; HUMAN; HUMAN CELL; LYMPHOCYTE ACTIVATION; LYMPHOCYTE DIFFERENTIATION; PERIPHERAL LYMPHOCYTE; PROMYELOCYTIC LEUKEMIA; PROTEIN ANALYSIS;

PHYTOLACCACEAE;

EID: 0031042236     PISSN: 10520295     EISSN: None     Source Type: Journal    
DOI: 10.3109/10520299709082204     Document Type: Article

References (32)

1. Baserga, R. 1984. Growth in size and cell DNA replication. Exp. Cell. Res. 151: 1-5.
2. Clevenger, C. V., Epstein, A. L. and Bauer, K. D. 1987. Modulation of the nuclear antigen p105 as a function of cell-cycle progression. J. Cell. Physiol. 130: 336-343.
3. Cooper, H. L. 1973. Effects of mitogens on the mitotic cycle: a biochemical evaluation of lymphocyte activation. In: Drugs and the Cell Cycle, Zimmerman, A. M., Padilla, G. M. and Cameron, I. L., eds. Academic Press, New York. pp. 137-181.
4. Crissman, H. A. and Steinkamp, J. A. 1973. Rapid, simultaneous measurement of DNA, protein and cell volume in single cells from large mammalian cell populations. J. Cell Biol. 59: 766-771.
5. Crissman, H. A. and Steinkamp, J. A. 1982. Rapid, one step staining procedures for analysis of cellular DNA and protein by single and dual laser flow cytometry. Cytometry 3: 84-90.
6. Crissman, H. A. and Steinkamp, J. A. 1987. Multivariate cell analysis: techniques for correlated measurements of DNA and other cellular constituents. In: Techniques in Cell Cycle Analysis, Gray, J. W. and Darzynkiewicz, Z., eds. Humana Press, Clifton, NJ. pp. 163-206.
7. Crissman, H. A., Van Egond, J., Holdrinet, R. S., Pennings, A. and Haanen, C. 1981. Simplified method for DNA and protein staining of human hematopoietic cell samples. Cytometry 2: 59-62.
8. Davidson, R. S. and Hilchenbach, M. M. 1990. The use of fluorescent probes in immunochemistry. Photochem. Photobiol. 52: 431-438.
9. Dyson, J. E., McLaughlin, J. B., Surrey, C. R., Simmons, D. M. and Daniel, J. 1987. Effects of hyperthermia, irradiation, and cytotoxic drugs on fluorescein isothiocyanate staining intensity for flow cytofluorometry. Cytometry 8: 26-34.
10. Engelhard, H. H. 1993. A quantitative analysis of modulations occurring in cellular c-myc protein content, percent S phase and growth balance under growth-inhibitory conditions. Cell. Mol. Biol. Oxf. 39: 607-620.
11. Engelhard, H. H., Krupka, J. L. and Bauer, K. D. 1991. Simultaneous quantification of c-myc oncoprotein, total cellular protein, and DNA content using multiparameter flow cytometry. Cytometry 12: 68-76.
12. Ennis, H. L. and Lubin, M. 1964. Cycloheximide: aspects of inhibition of protein synthesis in mammalian cells. Science 146: 1474-1476.
13. Ffrench, M., Bryon, P. A., Fiere, D., Vu Van, H., Gentilhomme, O., Adeleine, P. and Viala. J. J. 1985. Cell-cycle, protein content and nuclear size in acute myeloid leukemia. Cytometry 6: 47-53.
14. Freeman, D. A. and Crissman, H. A. 1975. Evaluation of six fluorescent protein stains for use in flow microfluorometry. Stain Technol. 50: 279-284.
15. Hardy, R. R., Hayakawa, K., Haaijman, J. and Herzenberg, L. A. 1982. B-cell subpopulations identified by two-colour fluorescence analysis. Nature 297: 589-591.
16. Herz, F., Czerniak, B., Deitch, D., Wersto, R. P., Simmons, D. A. and Koss, L. G. 1991. Protein expression in relation to the cell cycle of exponentially-growing human prostatic epithelial cells. Cell Proliferation 24: 321-30.
17. Hutter, K. J., Stohr, M. and Eipel, H. E. 1980. Simultaneous DNA and protein measurements of microorganisms. In: Flow Cytometry IV, Laerum, O. D., Lindmo, T. and Thorud, E., eds. Universitetsforlaget, Bergen, Norway, pp. 100-102.
18. Kapuscinski, J. and Darzynkiewicz, Z. 1990. Spectral properties of fluorochromes used in flow cytometry. In: Methods in Cell Biology, Vol. 33, Prescott, D. M., ed. Academic Press, New York. pp. 655-669.
19. Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265-275.
20. Masotti, L., Cavatorta, P., Avatabile, M., Barcellona, M. L., von Berger, J. and Ragusa, N. 1982. Characterization of 4′,6-diamidino-2 phenylindole (DAPI) as a fluorescent probe of DNA structure. Italian J. Biochem. (Engl. Ed.) 31: 90-99.
21. Mueller, W. and Gautier, F. 1975. Interactions of heteroaromatic compounds with nucleic acids: A-T specific nonintercalating DNA ligands. Eur. J. Biochem. 54: 384-394.
22. Park, C. H., Kimler, B. F. and Smith, T. K. 1985. Comparison of the supravital DNA dyes Hoechst 33342 and DAPI for flow cytometry and clonogenicity studies of human leukemic marrow cells. Exp. Hematol. 13: 1039-1043.
23. Parks, D. R., Hardy, R. R. and Herzenberg, L. A. 1983. Dual immunofluorescence-new frontiers in cell analysis and sorting. Immunol. Today 4: 145-150.
24. Roti Roti, J. L., Higashikubo, R., Blair, O. C. and Uygur, N. 1982. Cell-cycle position and nuclear protein content. Cytometry 3: 91-96.
25. Shapiro, H. M. 1988. Practical Flow Cytometry, 2nd ed. Alan R. Liss, New York pp. 126-138.
26. Stohr, M., Eipel, H., Goerttler, K. and Vogt-Schaden, M. 1977. Extended application of flow microfluorometry by means of dual laser excitation. Histochemistry 51: 305-313.
27. Stohr, M., Vogt-Schaden, M., Knobloch, M., Vogel, R. and Futterman, G. 1978. Evaluation of eight fluorochrome combinations for simultaneous DNA-protein flow analyses. Stain Technol. 53: 205-215.
28. Takahama, M. and Kagaya, A. 1988. Hematoporphyrin/DAPI staining: simplified simultaneous one-step staining of DNA and cell protein and trial application in automated cytological screening by flow cytometry. J. Histochem. Cytochem. 36: 1061-1067.
29. Thorsby, E. and Bratlie, A. 1970. A rapid method for preparation of pure lymphocyte suspension. In: Histocompatibility Testing, Terasaki, P. I., ed., Munksgaard, Copenhagen. pp. 655-659.
30. Titus, J. A., Haugland, R., Sharrow, S. O. and Segal, D. M. 1982. Texas red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies. J. Immunol. Methods 50: 193-204.
31. Traganos, F. 1984. Flow cytometry: principles and applications. Cancer Invest. 2: 149-163, 239-258.
32. Ulrich, W. 1992. Simultaneous measurement of DAPI-sulforhodamine 101 stained nuclear DNA and protein in higher plants by flow cytometry. Biotechnic & Histochemistry 67: 73-78.