The isolation and characterization of an immunomodulatory and anti-tumor polysaccharide preparation from Flammulina velutipes

Immunopharmacology

Volumn 35, Issue 3, 1997, Pages 255-263

  Leung, M Y K ^a   Fung, K P ^a   Choy, Y M ^a  

^a CHINESE UNIVERSITY OF HONG KONG   (Hong Kong)

Author keywords

anti tumor polysaccharide; Flammulina velutipes; immunostimulation; mushroom

Indexed keywords

ANTINEOPLASTIC AGENT; CYCLOHEXIMIDE; FLAMMULINA VELUTIPES EXTRACT; IMMUNOMODULATING AGENT; PLANT EXTRACT; POLYSACCHARIDE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTINEOPLASTIC ACTIVITY; ARTICLE; BLEEDING; CANCER GRAFT; CELL WALL; CONTROLLED STUDY; DRUG ISOLATION; DRUG STRUCTURE; FEMALE; HUMAN; IMMUNOMODULATION; INTRAPERITONEAL DRUG ADMINISTRATION; LYMPHOCYTE PROLIFERATION; MALE; MOLECULAR WEIGHT; MOUSE; MUSHROOM; PRIORITY JOURNAL; SARCOMA 180; SHRIMP; SPLEEN LYMPHOCYTE; VASODILATATION;

ADJUVANTS, IMMUNOLOGIC; ANIMALS; ANTINEOPLASTIC AGENTS; BASIDIOMYCOTA; CELL DIVISION; CELL WALL; FEMALE; LYMPHOCYTE ACTIVATION; LYMPHOCYTES; MALE; MICE; MICE, INBRED BALB C; MICE, INBRED ICR; MOLECULAR WEIGHT; NEOPLASM TRANSPLANTATION; POLYSACCHARIDES; SARCOMA 180; SPLEEN;

EID: 0031042159     PISSN: 01623109     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0162-3109(96)00157-9     Document Type: Article

References (36)

1. Berczi I. Neuroendocrine defence in endotoxin shock (a review), Acta Microbiol Hung 1993; 40: 265-302.
2. Bernheimer AW and Oppenheim JD. Some properties of flammutoxin from the edible mushroom Flammulina velutipes. Toxicon 1987; 25: 1145-1152.
3. Blaschek W, Kasbauer J, Kraus J and Franz G. Pythium aphanidermatum: culture, cell-wall composition and isolation and structure of antitumor storage and solubilised cell wall (1 → 3), (1 → 6)-β-D-glucans. Carbohydr Res 1992; 231: 239-307.
4. Blumenthal HJ. Reserve carbohydrates in Fungi. In: JE Smith and DR Berry Eds. The Filamentous Fungi, Vol. 11, Biosynthesis and Metabolism, Edward Arnold, 1978.
5. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248-254.
6. Chaplin MF and Kennedy JF. Uronic acid (Carbazole) assay. In: Carbohydrate Analysis - A practical approach. IRL Press, 1986; pp. 129-130.
7. Crop JK and Austen KF. Properties of glucans that activate the human alternative complement pathway and interact with the human monocyte β-glucan receptor. Immun 1985; 135: 3388-3393.
8. Dubois M, Gilles KA, Hamilton JK, Rebers PA and Smith F. Colorimetric method for the determination of sugars and related substances. Anal Chem 1956; 28: 350-356.
9. Fujimoto H, Nakayama M, Nakayama Y and Yamazaki M. Isolation and characterization of immunosuppressive components of three mushrooms, Pisolithus tinctorius, microporus flabelliforrnis and lenzites betulina. Chem Pharm Bull 1994, 42: 694-697.
10. Fung KP, Cheng CP, Luey LU, Loh SC, Choy YM and Lee CY. Effect of combined treatment of anti-inflammatory drug and mannoheptulose on the production of tumor necrosis factor and endotoxin shock in mice. Cancer Lett 1988; 39: 137-143.
11. Hussain RF, Nouri AME and Oliver RTD. A new approach for measurement of cytotoxicity using colorimetric assay. J Immun Method 1993; 160: 89-96.
12. Ikekawa T, Uehara N, Maeda Y, Nakaniski M and Fukuoka F. Antitumor activity of aqueous extracts of edible mushrooms. Cancer Res 1969; 29: 734-735.
13. Ikekawa T, Ideda Y, Yoshioka Y, Nakanishi K, Yokoyama E and Yamazaki E. Studies on antitumor polysaccharides of Flammulina velutipes (Curt. ex Fr.) Sing. II. The structure of EA 3 and further purification of EA 5. J pharmacobiodyn 1982, 5: 576-581.
14. Jacqueline S, Kabat EA and Morrison SL. Studies on mouse hybridomas secreting IgM or IgA antibodies to α(1→6)-linked dextran. Cell Immun 1981; 18: 831-846.
15. Koppel C. Clinical symtomatology and management of mushroom poisoning. Toxicon 1993; 31: 1513-1540.
16. Kraus J, Blaschek W, Schutz M and Franz G. Antitumor activity of cell wall β-1, 3/1,6-glucans from Phytophthora species. Planta Med 1992; 58: 39-42.
17. Liu WK, Ng TB, Sze SF and Tsui KW. Activation of peritoneal macrophages by polysaccharopeptide from the mushroom, Coriolus versicolor. Immunopharmacol 1993; 26: 139-146.
18. Maeda YY, Suga T, Watanabe ST, Rokutanda M and Chihara G. Induction of early acute phase serum factors associated with inflammation by Lentinan. In: T Aoki, E subura and I Urushizaki Eds. Manipulation of Host Defence Mechanisms, Excerpta Medica, 1984; APCS No. 38.
19. Molitoris HP, Mushrooms in medicine. Folia Microbiol 1994; 39: 91-98.
20. Mosier DE, Scher I, Ruhl H, Cohen PL, Zitron I and Paul WE. Activation of Normal and Defective B Lymphocytes by Thymus-Independent Antigens. In: JJ Oppenheim and DL Rosenstreich Eds. Mitogens in Immunobiology. Academic Press, 1976.
21. Nakanishi I. Demonstration of Curdlan-type polysaccharide and some other β-(1 → 3)-D-glucans in microorganisms with aniline blue. J Gen Appl Microbiol 1976; 22: 1-11.
22. Ohkuma T, Otagiri K, Ikekawa T and Tanaka S. Augmentation of antitumor activity by combined cryo-destruction of sarcoma 180 and protein-bound polysaccharide, EA6, isolated from Flammulina velutipes (Curt. ex Fr.) Sing. In ICR mice. J Pharmacobiodyn 1982; 5: 439-444.
23. Ohkuma T, Tanaka S and Ikekawa T. Augmentation of the host's immunity by combined cryodestruction of sarcoma 180 and administration of protein bound polysaccharide, EA 6, isolated from Flammulina velutipes (Curt. ex Fr.) Sing in ICR mice. J Pharmacobiodyn 1983; 6: 88-95.
24. Ohno N, Suzuki I, Oikawa S, Sato K, Miyazaki T and Yadomae T. Antitumor activity and structural characterization of glucans extracted from cultured fruit bodies of Grifola frondosa. Chem Pharm Bull 1984; 32: 1142-1151.
25. Otagiri K, Ohkuma T, Ikekawa T and Tanaka S. Intensification of antitumor immunity by protein-bound polysaccharide, EA6, derived from Flammulina velutipes (Curt. Ex Fr.) Sing. combined with murine leukemia L1210 vaccine in animal experiments. J Pharmacobiodzn 1983; 6: 96-104.
26. Solis PN, Wright CW, Anderson MM, Gupta MP and Philipson JD. A microwell cytotoxicity assay using Artemia Salina (Brine Shrimp). Planta Medica 1992; 59: 250-252.
27. Trevelyan WE, Proter DP and Harrison JS. Detection of sugars on paper chromatograms. Nature 1950; 166: 444-445.
28. Tsuchida K, Aoyagi Y, Odani S, Mita T and Isemura M. Isolation of a novel collagen binding protein from the mushroom, Hypsizigus marmoreus, which inhibits the Lewis lung carcinoma cell adhesion to type IV collagen. J Biol Chem 1995; 270: 1481-1484.
29. Wessels JGH, Mol PC, Sietsma JH and Vermerilen CA. Wall Structure, Wall Growth, and Fungal Cell Morphogenesis. In: PJ Kuhn, APJ Trinci, MJ Jung, MW Goosey, LG Copping Eds. Biochemistry of Cell Walls and Membranes in Fungi. Springer Verlag, 1990.
30. Wong CK, Leung KN, Fung KP, Pang PKT and Choy YM. Mitogenic and tumor necrosis factor producing activities of Pseudostellaria heterophylla. Int J Immunopharmacol 1992; 14: 1315-1320.
31. Wong CK, Leung KN, Fung KP and Choy YM, Immunomodulatory and anti-tumor polysaccharides from medicinal plants. J Int Med Res 1994a; 22: 299-312.
32. Wong CK, Leung KN, Fung KP and Choy YM. Effects of Pseudostellaria heterophylla on proliferation and differentiation of murine bone marrow cells. Immunopharmacol Immunotoxicol 1994b; 16: 71-84.
33. Wong CK, Leung KN, Fung MC, Fung KP and Choy YM. The induction of cytokine gene expression in murine peritoneal macrophages by Pseudostellaria heterophylla. Immunopharmacol Immunotoxicol 1994c; 16: 347-357.
34. Wong CK, Leung KN, Fung KP and Choy YM. The immunostimulating activities of anti-tumor polysaccharides from Pseudostellaria heterophylla. Immunopharmacol 1994d; 28: 47-54.
35. Wong CK, Leung KN, Fung KP, Pang PKT and Choy YM. Tumor necrosis factor eliciting fractions separated from Pseudostellaria heterophylla. Int J Immunopharmacol 1994e; 16: 271-277.
36. Yoshioka Y, Sano T and Ikekawa T. Studies on Antitumor Polysaccharides of Flammulina velutipes (Curt. ex Fr.) Sing. I. Chem Pharm Bull 1973; 21: 1772-1776.