Isolation and characterization of a genomic clone for the gene of an insect molting enzyme, chitinase

Insect Biochemistry and Molecular Biology

Volumn 27, Issue 1, 1997, Pages 37-47

  Choi, Hee Kyung ^a   Choi, Kyung Hyun ^a   Kramer, Karl J ^a,b   Muthukrishnan, Subbaratnam ^a  

^a KANSAS STATE UNIVERSITY   (United States)

^b AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

Chitinase; Chitinolytic enzyme; DNA sequence; Exon; Gene structure; Genomic clone; Intron; Manduca sexta; Molting enzyme; Tobacco hornworm

Indexed keywords

CHITINASE; COMPLEMENTARY DNA; DNA;

AMINO ACID SEQUENCE; ANIMAL; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; DNA PROBE; ENZYME ISOLATION; ENZYMOLOGY; GENE; GENETICS; GENOME; INTRON; LEPIDOPTERA; MOLECULAR CLONING; MOLECULAR GENETICS; MOLTING; NONHUMAN; NUCLEOTIDE SEQUENCE; SEQUENCE ANALYSIS; SOUTHERN BLOTTING; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BLOTTING, SOUTHERN; CHITINASE; CLONING, MOLECULAR; DNA; GENES, INSECT; GENOME; INTRONS; MANDUCA; MOLECULAR SEQUENCE DATA; MOLTING;

EID: 0031040174     PISSN: 09651748     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0965-1748(96)00066-5     Document Type: Article

References (29)

1. Altschul S. F., Gish W., Miller W., Myers E. W. and Lipman D. J. (1990) Basic local alignment search tool. J. Molec. Biol. 215, 403-410.
2. Bell R. and Joachim F. G. (1976) Techniques for rearing laboratory colonies of tobacco hornworms and pink bollworms. Ann. Entomol. Soc. Am. 69, 365-373.
3. Blaiseau P. L. and Lafay J. F. (1992) Primary structure of a chitinase encoding gene (chil) from the filamentous fungus Aphanocladium album: similarity to bacterial chitinase. Gene 120, 243-248.
4. Boot R. G., Renkema G. H., Strijland A., Van Zonneveld A. J. and Aerts J. M. F. G. (1995) Cloning of a cDNA encoding chitotriosidase, a human chitinase produced by macrophages. J. Biol. Chem. 270, 26252-26256.
5. Cherbas L., Lee K. and Cherbas P. (1991) Identification of ecdysone responsive element by analysis of the Drosophila Eip 28/29 gene. Genes Devl. 5, 120-131.
6. Chrispeels M. J. and Raikhel N. V. (1991) Lectins, lectin genes, and their role in plant defense. Plant Cell 3, 1-9.
7. Ding X. (1995) Manduca chitinase-mediated resistance to tobacco budworm (Heliothis virescens) and tobacco hornworm (Manduca sexta) larvae in transgenic tobacco plants. Ph.D. Dissertation, Kansas State University, Manhattan, 73 pp.
8. Feinberg A. P. and Vogelstein B. (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analyt. Biochem. 132, 6-13.
9. Flach J., Pilet P. E. and Jolles P. (1992) What's new in chitinase research? Experientia 48, 701-716.
10. Fuhrman J. A. (1995) Filarial chitinases. Parasitol. Today 11, 259-261.
11. Fuhrman J. A., Lane W. S., Smith R. F., Piessens W. F. and Perler F. B. (1992) Transmission-blocking antibodies recognize microfilarial chitinase in brugian lymphatic filariasis. Proc. Natn. Acad. Sci. U.S.A. 89, 1548-1552.
12. Fukamizo T. and Kramer K. J. (1985a) Mechanism of chitin oligosaccharide hydrolysis by the binary enzyme chitinase system in insect moulting fluid. Insect Biochem. 15, 1-7.
13. Fukamizo T. and Kramer K. J. (1985b) Mechanism of chitin hydrolysis by the binary chitinase system in insect moulting fluid. Insect Biochem. 15, 141-145.
14. Gaynor J. J. and Unkenholz K. M. (1989) Sequence analysis of a genomic clone encoding an endochitinase from Solanum tuberosum. Nucl. Acids Res. 17, 5855-5857.
15. Gopalakrishnan B., Muthukrishnan S. and Kramer K. J. (1995) Baculovirus-mediated expression of a Manduca sexta chitinase gene: properties of the recombinant protein. Insect Biochem. Molec. Biol. 25, 255-265.
16. Hakala B. E., White C. and Recklies A. D. (1993) Human cartilage gp-39, a major secretory product of articular chrondrocytes and synovial cells, is a mammalian member of a chitinase protein family. J. Biol. Chem. 268, 25803-25810.
17. Kirkpatrick R. B., Matico R. E., McNulty D. E., Strickler J. E. and Rosenberg M. (1995) An abundantly secreted glycoprotein from Drosophila melanogaster, is related to mammalian secretory proteins produced in rheumatoid tissues and by activated macrophages. Gene 153, 147-154.
18. Koga D., Fujimoto H., Funakoshi T., Utsumi T. and Ide A. (1989) Appearance of chitinolytic enzymes in integument of Bombyx mori during the larval-pupal transformation. Evidence for zymogenic forms. Insect Biochem. 19, 123-128.
19. Koga D., Funakoshi T., Mizuki K., Ide A., Kramer K. J., Zen K. C., Choi H. K. and Muthukrishnan S. (1992) Immunoblot analysis of chitinolytic enzymes in integument and molting fluid of the silkworm, Bombyx mori, and the tobacco hornworm, Manduca sexta. Insect Biochem. Molec. Biol. 22, 305-311.
20. Kramer K. J., Corpuz L., Choi H. K. and Muthukrishnan S. (1993) Sequence of a cDNA and expression of the gene encoding epidermal and gut chitinases of Manduca sexta. Insect Biochem. Molec. Biol. 23, 691-701.
21. Krishnan A., Nair P. N. and Jones D. (1994) Isolation, cloning and characterization of a new chitinase stored in active form in chitin-lined venom reservoir. J. Biol. Chem. 269, 20971-20976.
22. Kuranda M. J. and Robbins P. W. (1991) Chitinase is required for cell separation during growth of Saccharomyces cerevisiae. J. Biol. Chem. 266, 19758-19767.
23. Malette B., Paquette Y., Merlen Y. and Bleau G. (1995) Oviductins possess chitinase-and mucin-like domains: a lead in the search for the biological function of these oviduct-specific ZP-associating glycoproteins. Molec. Reprod. Devl. 41, 384-397.
24. Renkema G. H., Boot R. G., Muijsers A. O., Donker-Koopman W. E. and Aerts J. M. F. G. (1995) Purification and characterization of human chitotriosidase, a novel member of the chitinase family of proteins. J. Biol. Chem. 270, 2198-2202.
25. Rogers S. G. and Weiss B. (1980) Exonuclease III of Escherichia coli, K-12, an AP endonuclease. Meths. Enzymol. 65, 201-210.
26. Sambrook J., Fritsch E. F. and Maniatis T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Publications, New York.
27. Shinshi H., Neuhaus J. M., Ryals J. and Meins F. (1990) Structure of a tobacco endochitinase gene: evidence that different chitinase genes can arise by transposition of sequences encoding a cysteine-rich domain. Pl. Molec. Biol. 14, 357-368.
28. Southern E. M. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Molec. Biol. 98, 503-517.
29. Watanabe T., Kobori K., Miyashita K., Fujii T., Sakai H., Uchida M. and Tanaka H. (1993) Identification of glutamic acid 204 and aspartic acid 200 in chitinase A1 of Bacillus circulons, WL-12 as essential residues for catalytic activity. J. Biol. Chem. 268, 18567-18572.