Expression of transferrin receptors in endothelial cells transfected by electroporation

European Journal of Cell Biology

Volumn 72, Issue 1, 1997, Pages 79-89

  Heltianu, Constantina ^a   Serban, Geo ^a   Alexandru, Valentina ^a   Gafencu, Anca ^a   Simionescu, Nicolae ^a   Simionescu, Maya ^a  

^a INSTITUTE OF CELLULAR BIOLOGY AND PATHOLOGY NICOLAE SIMIONESCU   (Romania)

Author keywords

Electroporation; Endothelium; Transfection; Transferrin receptor

Indexed keywords

ANTIBIOTIC G 418; HORSERADISH PEROXIDASE; IRON BINDING PROTEIN; TRANSFERRIN RECEPTOR;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL MEMBRANE; CELL NUCLEUS MEMBRANE; CELL ORGANELLE; CELL ULTRASTRUCTURE; CONTROLLED STUDY; CYTOCHEMISTRY; CYTOPLASM VESICLE; DNA TRANSFECTION; ELECTROPORATION; ENDOCYTOSIS; ENDOSOME; ENDOTHELIUM CELL; EXOCYTOSIS; GOLGI COMPLEX; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN SYNTHESIS; REPORTER GENE; ROUGH ENDOPLASMIC RETICULUM;

ANIMALIA; ARMORACIA RUSTICANA;

ANIMALS; AORTA; CALCIUM PHOSPHATES; CATTLE; CELLS, CULTURED; ELECTROPORATION; ENDOTHELIUM, VASCULAR; GENE EXPRESSION; HUMANS; RECEPTORS, TRANSFERRIN; RECOMBINANT FUSION PROTEINS; TRANSFECTION;

EID: 0031037455     PISSN: 01719335     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (47)

1. Baum, C., P. Forster, S. Hegewisch-Becker, K. Harberg: An optimised electroporation protocol applicable to a wide range of cell lines. BioTechniques 17, 1058-1062 (1994).
2. Cerneus, D. P., A. van der Ende: Apical and basolateral transferrin receptors in polarised BeWo cells recycle through separate endosomes. J. Cell Biol. 114, 1149-1158 (1991).
3. Connolly, C. N., C. E. Futter, A. Gibson, C. R. Hopkins, D. F. Cutler: Transport into and out of the Golgi complex studied by transfecting cells with cDNAs encoding horseradish peroxidase. J. Cell Biol. 127, 641-652 (1994).
4. Fickling, S. A., J. A. Tooze, G. St. J. Whitley: Characterisation of human umbilical vein endothelial cell lines produced by transfection with the early region of SV40. Exp. Cell Res. 201, 517-521 (1992).
5. Fitzgerald, U., J. S. McLean, C. MacDonald: Production of immortal human endothelial cell lines by strontium phosphate transfection and electroporation of SV40 sequences. In Vitro Cell Dev. Biol. 30A, 236-242 (1994).
6. Futter, C. E., C. N. Connolly, D. F. Cutler, C. R. Hopkins: Newly synthesised transferrin receptors can be detected in the endosome before they appear on the cell surface. J. Biol. Chem. 270, 10999-11003 (1995).
7. Ghitescu, L., A. Fixman, M. Simionescu, N. Simionescu: Specific binding sites for albumin restricted to plasmalemmal vesicles of continuous capillary endothelium: Receptor mediated transcytosis. J. Cell Biol. 102, 1304-1311 (1986).
8. Ghosh, R. N., D. L. Gelman, F. R. Maxfield: Quantification of low density lipoprotein and transferrin endocytic sorting in HEp 2 cells using confocal microscopy. J. Cell Sci. 107, 2177-2189 (1994).
9. 2-macroglobulin. Atherosclerosis 34, 391-405 (1979).
10. Hopkins, C. R., J. S. Trowbridge: Internalization and processing of transferrin and the transferrin receptor in human carcinoma H 431 cells. J. Cell Biol. 97, 508 (1983).
11. Hopkins, C. R.: The appearance and internalisation of transferrin receptors at the margins of spreading human tumour cells. Cell 40, 199-208 (1985).
12. Hopkins, C. R., A. Gibson, M. Shipman, K. Miller: Movement of internalised ligand-receptor complexes along a continuos endosomal reticulum. Nature 346, 335-339 (1990).
13. Hormia, M., V.-P. Lehto, I. Virtanen: Intracellular localization of factor VIII-related antigen and fibronectin in cultured human endothelial cells: evidence for divergent routes in intracellular translocation. Eur. J. Cell Biol. 33, 217-228 (1984).
14. Jaffe, E. A., W. Hoyer, R. L. Nachman: Synthesis of antihemophilic factor antigen by cultured human endothelial cells. J. Clin. Invest. 52, 2757-2764 (1973).
15. Jefferies, W. A., M. R. Brandon, S. V. Hunt, A. F. Williams, K. C. Gater, D. Y. Mason: Transferrin receptors on endothelium of brain capillaries. Nature 312, 162-163 (1984).
16. Jinga, V. V., I. Bogdan, J. Fruchter: Experimental model for the quantitative estimation of transendothelial transport in vitro; a two-compartment system. Rev. Roum. Morphol. Physiol. 23, 227-230 (1986).
17. Lassalle, P., C. La Grou, V. Delneste, J. Sanceau, J. Coll, G. Torpier, J. Wientzerbin, D. Stehelin, A. B. Tonnel, A. Carpon: Human endothelial cells transfected by SV 40T antigens characterization and potential use as a source of normal endothelial factors. Eur. J. Immunol. 22, 425-431 (1992).
18. Liu, S. Q., I. Yoshihiro, Y. Imanishi: Cell growth on immobilized cell growth factor. 9. Covalent immobilization of insulin, transferrin, and collagen to enhance growth of bovine endothelial cells. J. Biomed. Materials Res. 27, 909-915 (1993).
19. Manduteanu, I., D. Popov, A. Radu, M. Simionescu: Calf cardiac valvular endothelial cells in culture: production of glycosaminoglycans, prostacyclin and fibronectin. Atherosclerosis 72, 77-82 (1988).
20. Maxwell, I. H., F. Maxwell: Electroporation of mammalian cells with a firefly luciferase expression plasmid: kinetics of transient expression differ markedly among cell types. DNA 7, 557-562 (1988).
21. Milici, A. J., N. E. Watrous, H. Stukenbrok, G. E. Palade: Transcytosis of albumin in capillary endothelium. J. Cell Biol. 105, 2603-2612 (1987).
22. Neish, A. S., A. J. Williams, H. J. Palmer, M. Z. Witley, T. Collins: Functional analysis of the human vascular cell adhesion molecule 1 promoter. J. Exp. Med. 176, 1583-1593 (1992).
23. Podbilewcz, B., I. Mellman: ATP and cytosol requirements for transferrin recycling in intact and disrupted MDCK cells. EMBO J. 9, 3477-3487 (1990).
24. Rao, C. D., M. Pech, K. C. Robbins, S. A. Aaronson: The 5′ untranslated sequence of the c-sis/platelet-derived growth factor 2 transcript is a potent translational inhibitor. Mol. Cell. Biol. 8, 284-292 (1988).
25. Raub, T. J., C. R. Newton: Recycling kinetics and transcytosis of transferrin in primary cultures of bovine brain microvessel endothelial cells. J. Cell. Physiol. 149, 141-151 (1991).
26. Rech, E. L., E. S. Alves, M. R. Davey: Electroporation: a circuit diagram and computer program for assessment of physical parameters on eucaryotic cells. Technique - A J. of Methods in Cell and Mol. Biol. 1, 125-129 (1989).
27. Roberts, R. L., A. Sandra: Transport of transferrin across the blood-thymus barrier in young rats. Tissue and Cell 26, 757-766 (1994).
28. Rothenberger, S., B. J. Iacopetta, L. C. Kuhn: Endocytosis of the transferrin receptor requires the cytoplasmic domain but not its phosphorylation site. Cell 49, 423-431 (1987).
29. Ryan, V. S., R. Zhong, B. A. Hayes, G. Visner, M. L. Sauther: Regulation of endothelin-1 expression in normal and transfected endothelial cells. J. Cardiovasc. Pharmacol. 22, S38-S41 (1993).
30. Sambrook, J., E. F. Fritsch, T. Maniatis: Molecular Cloning. A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press 1989.
31. Schwachtgen, J. L., V. Ferreira, D. Meyer, D. Kerbirion-Nabias: Optimisation of transfection of human endothelial cells by electroporation. BioTechniques 17, 882-887 (1994).
32. Shah, D., W. C. Shen: The establishment of polarity and enhanced transcytosis of transferrin receptors in enterocyte-like CaCo-2 cells. J Drug Target 2, 93-99 (1994).
33. Shima, D. T., K. B. Saunders, A. Gougos, P. A. D'Amore: Alterations in gene expression associated with changes in the state of endothelial differentiations. Differentiation 58, 217-226 (1995).
34. Simionescu, M.: Transendothelial movement of large molecules in the microvasculature. In: A. P. Fischman, E. M. Renkin (eds): Pulmonary Edema, pp. 39-52. Am. Physiol. Soc. Bethesda 1979.
35. Simionescu, M., N. Simionescu: Endothelial transport of macromolecules: transcytosis and endocytosis. A look from cell biology. In: M. Simionescu, N. Simionescu (eds.): Cell Biology Reviews. Vol. 25. pp. 1-79. Springer International. Heidelberg, Berlin 1991.
36. Simionescu, N.: Enzymatic tracers in the study of vascular permeability. J. Histochem. Cytochem. 27, 1120-1130 (1979).
37. Simionescu, N.: Transcytosis and traffic of membranes in the endothelial cells. In: H. G. Schweiger (ed): International Cell Biology. pp. 657-672. Springer-Verlag. Berlin 1981.
38. Soda, R., M. Tavassoli: Liver endothelium and not hepatocytes or kupffer cells have transferrin receptors. Blood 63, 270-276 (1984).
39. Stinchombe, J. C., H. Nomoto, D. F. Cutler, C. R. Hopkins: Anterograde and retrograde traffic between the rough endoplasmic reticulum and the Golgi complex. J. Cell Biol. 131, 1387-1401 (1995).
40. Takebe, Y. M. Seiki, J. I. Fujisava, P. Hoy, K. Yokota, K. Arai, M. Yoshida, N. Arai: SR-alpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat. Mol. Cell. Biol. 8, 466-472 (1988).
41. Teissie, J., M.-P. Rols: Manipulation of cell cytoskeleton affects the lifetime of cell membrane electropermeabilization. Ann. N. Y. Acad. Sci. 691, 98-110 (1994).
42. Toose, J., M. Hollinshead: In AtT20 and HeLa cells brefeldin A induces the fusion of tubular endosomes and changes their distribution and some of their endocytic properties. J. Cell Biol. 118, 813-830 (1992).
43. Van Gelder, W., M. I. E. Huijskes-Heins, J. P. van Dijk, M. I. Cleton-Soeteman, H. G. van Eijk: Quantification of different transferrin receptor pools in primary cultures of porcine blood-brain endothelial cells. J. Neurochem. 64, 2708-2715 (1995).
44. Van Zonneveld, A.-J., S. A. Curriden, D. J. Loskutoff: Type 1-plasminogen activator inhibitor gene. Functional analysis and glycocorticoid regulation of its promoter. Proc. Natl. Acad. Sci. USA. 85, 5525-5529 (1988).
45. Vasile, E., F. Antohe, M. Simionescu, N. Simionescu: Transport pathways of beta-VLDL by aortic endothelium of normal and hypercholesterolemic rabbits. Atherosclerosis 75, 195-210 (1989).
46. Voyta, J. C., D. P. Via, E. C. Butterfield, B. R. Zetter: Identification and isolation of endothelial cells based on their increased uptake of acetylated LDL. J. Cell Biol. 99, 2034-2040 (1984).
47. Wolf, H., M. P. Rols, E. Boldt, E. Neumann, J. Teissie: Control by pulse parameters of electric field-mediated gene transfer in mammalian cells. Biophysical J. 66, 524-531 (1994).