Expression of microtubule-associated protein-2a and other novel microtubule-associated protein-2 transcripts in human fetal spinal cord

Journal of Neurochemistry

Volumn 68, Issue 1, 1997, Pages 383-391

  Kalcheva, Nellie ^a   Weidenheim, Karen M ^a   Kress, Yvonne ^a   Shafit Zagardo, Bridget ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

Development; Human fetal spinal cord; Microtubule associated protein 2b+8; Microtubule associated protein 2a; Synaptogenesis

Indexed keywords

MICROTUBULE ASSOCIATED PROTEIN 2;

ARTICLE; FETUS; FETUS DEVELOPMENT; HUMAN; HUMAN TISSUE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; RNA SPLICING; SPINAL CORD; SYNAPTOGENESIS;

EID: 0031037198     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1471-4159.1997.68010383.x     Document Type: Article

References (26)

1. Albala J. S., Kalcheva N., and Shafit-Zagardo B. (1993) Characterization of the transcripts encoding two isoforms of human microtubule-associated protein-2 (MAP-2). Gene 136, 377-378.
2. Albala J. S., Kress Y., Liu W.-K., Weidenheim K., Yen S.-H. C., and Shafit-Zagardo B. (1995) Human microtubule-associated protein-2c localizes to dendrites and axons in fetal spinal motor neurons. J. Neurochem. 64, 2480-2490.
3. Bernhardt R. and Matus A. (1984) Light and electron microscopic studies of the distribution of microtubule-associated protein 2 in rat brain: a difference between the dendritic and axonal cytoskeletons. J. Comp. Neurol. 226, 203-221.
4. Caceres A., Binder L. I., Payne M. R., Bender P., Rebhun L., and Steward O. (1984) Differential subcellular localization of tubulin and the microtubule-associated protein MAP2 in brain tissue as revealed by immunohistochemistry with monoclonal antibodies. J. Neurosci. 4, 394-410.
5. Caceres A., Mautino J., and Kosik K. S. (1992) Suppression of MAP2 in cultured cerebellar macroneurons inhibits minor neurite formation. Neuron 9, 607-618.
6. Chen J., Kanai Y., Cowan N. J., and Hirokawa N. (1992) Projection domains of MAP-2 and tau determine the spacing between microtubules in dendrites and axons. Nature 360, 674-677.
7. Chung W. J., Kindler S., Seidenbecher C., and Garner C. C. (1996) MAP-2a, an alternatively spliced variant of microtubule-associated protein 2. J. Neurochem. 66, 1273-1281.
8. DeCamilli P., Miller P. E., Navone F., Theurkauf W. E., and Vallee R. B. (1984) Distribution of microtubule-associated protein 2 in the nervous system of the rat studied by immunofluorescence. Neuroscience 11, 817-846.
9. Gray E. G. (1959) Axo-somatic and axo-dendritic synapses of the cerebral cortex. An electron microscopy study. J. Anat. 93, 420-433.
10. Huber G. and Matus A. (1984) Differences in the cellular distribution of two microtubule-associated proteins, MAP-1 and MAP-2, in rat brain. J. Neurosci. 4, 151-160.
11. Kalcheva N. and Shafit-Zagardo B. (1995) Three unique 5′ untranslated regions are spliced to common coding exons of high- and low-molecular-weight microtubule-associated protein-2. J. Neurochem. 65, 1472-1480.
12. Kalcheva N., Albala J. S., O'Guin K., Rubino H., Garner C. C., and Shafit-Zagardo B. (1995) Genomic structure of human microtubule-associated protein-2 (MAP2) and characterization of newly identified isoforms. Proc. Natl. Acad. Sci. USA 92, 10894-10898.
13. Laemmli U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
14. Mattiace L. A., Davies P., and Dickson D. W. (1991) Detection of HLA-DR on microglia in the human brain is a function of both clinical and technical factors. Am. J. Pathol. 136, 1101-1114.
15. Matus A. (1988) Microtubule-associated proteins: their potential role in determining neuronal morphology. Annu. Rev. Neurosci. 11, 29-44.
16. Neve R. L., Harris P., Kurnit D. M., Kosik K. S., and Donlon T. A. (1986) Identification of cDNAs for human tau and the chromosomal localization of the genes for tau and MAP-2. Mol. Brain Res. 1, 271-280.
17. Papandrikopoulou A., Doll T., Tucker R. P., Gamer C. C., and Matus A. (1989) Embryonic MAP2 lacks the cross-linking side arm sequences and dendritic targeting signal of adult MAP2. Nature 340, 650-652.
18. Riederer B. and Matus A. (1985) Differential expression of distinct microtubule-associated proteins during brain development. Proc. Natl. Acad. Sci. USA 82, 6006-6009.
19. Schoenen J. and Faull R. L. M. (1990) Spinal cord: cytoarchitectural, dendroarchitectural, and myeloarchitectural organization, in The Human Nervous System (Paxinos G., ed), pp. 19-51. Academic Press, San Diego.
20. Shafit-Zagardo B., Kalcheva N., Dickson D., Davies P., and Kress Y. (1997) Distribution and subcellular localization of high-molecular-weight microtubule-associated protein-2 expressing exon 8 in brain and spinal cord. J. Neurochem. (in press).
21. Sharma N., Kress Y., and Shafit-Zagardo B. (1994) Antisense MAP-2 oligonucleotides induce changes in microtubule assembly and neuritic elongation in pre-existing neurites of rat cortical neurons. Cell Motil. Cytoskeleton 27, 234-247.
22. Sidman R. L. and Rakic P. (1982) Development of the human central nervous system, in Histology and Histopathology of the Nervous System (Haymaker W. and Adams R. D., eds), pp. 3-145. Charles C. Thomas, Springfield, Illinois.
23. Tohyama T., Lee V. M.-Y., Rorke L. B., and Trojanowski J. Q. (1991) Molecular milestones that signal axonal maturation and the commitment of human spinal cord precursor cells to the neuronal or glial phenotype in development. J. Comp. Neurol. 310, 285-299.
24. Towbin H., Staehelin T., and Gordon J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350-4354.
25. 2 epitopes revealed with monoclonal antibodies in microwave-denatured human nervous system tissue. J. Neurosci. Methods 29, 171-180.
26. Viereck C., Tucker R. P., Binder L. I., and Matus A. (1988) Phylogenetic conservation of brain microtubule-associated proteins MAP2 and tau. Neuroscience 26, 893-904.