Optimization of the isolation and effective use of mRNA from rat mast cells

Journal of Immunological Methods

Volumn 201, Issue 2, 1997, Pages 207-214

  Gilchrist, M ^a   MacDonald, A J ^a   Neverova, I ^a   Ritchie, B ^a   Befus, A D ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

Author keywords

heparinase; histidine decarboxylase; mast cell; paper chromatography; RNA; RT PCR

Indexed keywords

BETA ACTIN; HEPARIN; HEPARIN LYASE; HISTIDINE DECARBOXYLASE; MESSENGER RNA;

ANIMAL CELL; ARTICLE; CELL LINE; MALE; MAST CELL; NONHUMAN; PAPER CHROMATOGRAPHY; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

ANIMALS; CELLS, CULTURED; HEPARIN; HEPARIN LYASE; MALE; MAST CELLS; METHODS; PERITONEAL CAVITY; POLYMERASE CHAIN REACTION; POLYSACCHARIDE-LYASES; RATS; RATS, SPRAGUE-DAWLEY; RNA, MESSENGER;

ANIMALIA;

EID: 0031036890     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(96)00230-X     Document Type: Article

References (24)

1. Befus, A.D. (1994) Inflammation: mast cells. In: P.L. Ogra, J. Mestecky, M. Lamm, W. Strober, J. McGhee and J. Bienenstock (Eds.), Handbook of Mucosal Immunology. Academic Press, San Diego, CA, p. 307.
2. Befus, A.D. and Bienenstock, J. (1979) Immunologically-mediated intestinal mastocytosis in Nippostrongylus brasiliensis infected rats. Immunology 38, 95.
3. Benfey, P.N., Yin, F.H. and Leder, P. (1987) Cloning of the mast cell protease, RMCPII. Evidence for cell-specific expression and a multi-gene family. J. Biol. Chem. 262, 5377.
4. Beutler, E., Gelbart, T. and Kühl, W. (1990) Interference of heparin with the polymerase chain reaction. BioTechniques 9, 166.
5. Bissonnette, E.Y., Chin, B. and Befus, A.D. (1995) Interferons differentially regulate histamine and TNF-a in rat intestinal mucosal mast cells. Immunology 86, 12.
6. Chan, B.M.C., Ra, C., Hu, W., McNeill, K., Miller, H.R.P., Befus, D., Kinet, J.P. and Froese, A. (1990) Characterization of rat tissue cultured mast cells. Eur. J. Immunol. 20, 185.
7. Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156.
8. Enciso, J.A., Bissonnette, E.Y. and Befus, A.D. (1996) Regulation of mRNA levels of TNF-alpha and the alpha chain of the high-affinity receptor for IgE in mast cells by IFN-gamma and alpha/beta. Int. Arch. Allergy Immunol. 110, 114.
9. Fujimaki, H., Lee, T.D.G., Swieter, M., Saito, A., Tamaoki, T. and Befus, A.D. (1988) Isolation and in vitro translation of mRNA from rat peritoneal mast cells and rat basophilic leukemia cells. J. Immunol. Methods 114, 219.
10. Galli, S.J. (1993) New concepts about the mast cell. New Engl. J. Med. 328, 257.
11. Gordon, J.R. and Galli, S.J. (1990) Mast cells as a source of both preformed and immunologically inducible TNF-α cachectin. Nature 346, 274.
12. Imai, H., Yamada, O., Morita, S., Suehiro, S. and Kurimura, T. (1992) Detection of HIV-1 RNA in heparinized plasma of HIV-1 seropositive individuals. J. Virol. Methods 27, 203.
13. Izraeli, S., Pfleiderer, C. and Lion, T. (1991) Detection of gene expression by PCR amplification of RNA derived from frozen heparinized whole blood. Nucleic Acids Res. 19, 6051.
14. Jordana, M. (1993) Mast cells and fibrosis - Who's on first? Am. J. Respir. Cell Mol. Biol. 8, 7.
15. Kormanec, J. and Farkasovsky, M. (1993) Simple and reliable determination of radioactive precursors incorporation into nucleic acids in-vitro. BioTechniques 15, 400.
16. Oberhauser, A.F., Balan, V., Fernandez-Badilla, C.L. and Fernandez, J.M. (1994) RT-PCR cloning of Rab3 isoforms expressed in peritoneal mast cells. FEBS Lett. 339, 171.
17. Roche, W.R. (1985) Mast cells and tumors: the specific enhancement of tumor proliferation in vitro. Am. J. Pathol. 119, 57.
18. Shanahan, F., Denburg, J., Fox, J., Bienenstock, J. and Befus, A.D. (1985) Effects of neuroenteric peptides on histamine release. J. Immunol. 135, 1331.
19. Tantravahi, R.V., Stevens, R.L., Austen, K.F. and Weis, J.H. (1986) A single gene in mast cells encodes the core peptides of heparin and chondroitin sulfate proteoglycans. Proc. Natl. Acad. Sci. USA 83, 9207.
20. Trabucchi, E., Radaelli, E., Marazzi, M., Foschi, D., Musazzi, M., Veronesi, A.M. and Montorsi, W. (1988) The role of mast cells in wound healing. Int. J. Tiss. Reac. X, 367.
21. Tsai, M., Miyamoto, M., Tam, S.-Y., Wang, Z.-S. and Galli, S.J. (1995) Detection of mouse mast cell-associated protease mRNA: Heparinase treatment greatly improves RT-PCR of tissues containing mast cell heparin. Am. J. Pathol. 146, 335.
22. Warbrick, E.V., Taylor, A.M., Botchkarev, V.A. and Coleman, J.W. (1995) Rat connective tissue-type mast cells express MHC class II: up-regulation by IFN-γ. Cell. Immunol. 163, 222.
23. Williams, C.M.W. and Coleman, J.W. (1995) Induced expression of mRNA for IL-5, IL-6, TNF-α, MIP-2 and IFN-γ in immunologically activated rat peritoneal mast cells: inhibition by dexamethasone and cyclosporin A. Immunology 86, 244.
24. Yurt, R.W., Leid, R.W. and Austen, K.F. (1977) Native heparin from rat peritoneal mast cells. J. Biol. Chem. 252, 518.