Expression of a lipocalin in Pichia pastoris: Secretion, purification and binding activity of a recombinant mouse major urinary protein

FEBS Letters

Volumn 401, Issue 1, 1997, Pages 73-77

  Ferrari, E ^a   Lodi, T ^a   Sorbi, R T ^a   Tirindelli, R ^a   Cavaggioni, A ^b   Spisni, A ^a  

^a UNIVERSITY OF PARMA   (Italy)

^b UNIVERSITY OF PADOVA   (Italy)

Author keywords

Heterologous expression; Major urinary protein; Pichia pastoris

Indexed keywords

LIPOCALIN; SECRETORY PROTEIN;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ENDOMYCETALES; GENE EXPRESSION; ION EXCHANGE CHROMATOGRAPHY; MOUSE; NONHUMAN; OLFACTORY SYSTEM; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FOLDING; PROTEIN PURIFICATION;

ALPHA-GLOBULINS; AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; GENETIC VECTORS; MICE; MOLECULAR SEQUENCE DATA; PICHIA; PROTEINS; RECOMBINANT PROTEINS;

ANIMALIA; PICHIA PASTORIS; SACCHAROMYCETALES;

EID: 0031033438     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-5793(96)01436-6     Document Type: Article

References (28)

1. Clark, A.J., Ghazal, P., Bingham, R.W., Barrett, D. and Bishop, J.O. (1985) EMBO J. 12, 3159-3165.
2. Clissold, P.M., Hainey, S. and Bishop, J.O. (1984) Biochem. Genet. 22, 379-387.
3. Flower, D.R., North, A.C.T. and Attwood, T.K. (1993) Protein Sci. 2, 753-761.
4. Flower, D.R. (1995) J. Mol. Recogn. 8, 185-199.
5. Pelosi, P. (1995) Crit. Rev. Biochem. Mol. Biol. 29, 199-228.
6. Cowan, S.W., Newcomer, M.E. and Jones, T.A. (1990) Proteins Struct. Funct. Genet. 8, 44-61.
7. Branden, C. and Tooze, J. (1991) Antiparallel β-structures. In: Introduction to Protein Structure. Garland, New York.
8. Sivaprasadarao, A., Boudjelal, M. and Findlay, J.B.C. (1993) Biochem. Soc. Trans. 21, 619-622.
9. Zanotti, G., Marcello, M., Malpeli, G., Folli, C., Sartori, G. and Berni, R. (1994) J. Biol. Chem. 26, 29613-29620.
10. Bacchini, A., Gaetani, E. and Cavaggioni, A. (1992) Experientia 48, 419-421.
11. Robertson, D.H.L., Beynon, R.J. and Evershed, R.P. (1993) J. Chem. Ecol. 19, 1405-1416.
12. Mucignat-Caretta, C., Caretta, A. and Cavaggioni, A. (1995) J. Physiol. 486, 517-522.
13. Kaneko, N., Debski, E.A., Wilson, M.C. and Whitten, W.K. (1980) Biol. Reprod. 22, 873-878.
14. Keverne, E.B. (1983) Trends Neurosci. 6, 381-384.
15. Cregg, J.M., Tschopp, J.F., Stillman, C., Siegel, R., Akong, M., Craig, W.S. et al. (1987) Bio/Technology 5, 479-485.
16. Digan, M.E., Lair, S.V., Brierley, R.A., Siegel, R.S., Williams, M.E., Ellis, S.B. et al. (1989) Bio/Technology 7, 160-164.
17. Cregg, J.M., Vedvick, T.S. and Raschke, W.C. (1993) Bio/Technology 11, 905-910.
18. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edn., Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
19. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. and Struhl, K. (1990) Current Protocols in Molecular Biology, Greene/Wiley-Interscience, New York.
20. Cavaggioni, A., Findlay, J.B.C. and Tirindelli, R. (1990) Comp. Biochem. Physiol. 96B, 513-520.
21. Shahan, K., Gilmartin, M. and Derman, E. (1987) Mol. Cell. Biol. 7, 1938-1946.
22. McIntosh, I. and Bishop, J.O. (1989) Mol. Cell. Biol. 9, 2202-2207.
23. Clissold, P.M. and Bishop, J.O. (1982) Gene 18, 211-220.
24. Johnson, D., Al-Shawi, R. and Bishop, J.O. (1995) J. Mol. Endocrinol. 14, 21-34.
25. Clark, A.J., Chave-Cox, A., Ma, X. and Bishop, J.O. (1985) EMBO J. 4, 3167-3171.
26. Cavener, D.R. and Stuart, C.R. (1991) Nucleic Acids Res. 19, 3185-3192.
27. Evershed, R.P., Robertson, D.H.L., Beynon, R.J. and Green, B. (1993) Rapid Commun. Mass Spectrom. 7, 882-886.
28. Robertson, D.H.L., Cox, K.A., Gaskell, S.J., Evershed, R.P. and Beynon, R.J. (1996) Biochem. J. 316, 265-272.