In situ nucleic acid hybridization of pyruvate dehydrogenase complex-E2 in primary biliary cirrhosis: Pyruvate dehydrogenase complex-E2 messenger RNA is expressed in hepatocytes but not in biliary epithelium

Hepatology

Volumn 25, Issue 1, 1997, Pages 27-32

  Harada, Kenichi ^a,b   Van De Water, Judy ^a   Leung, Patrick S C ^a   Coppel, Ross L ^c   Nakanuma, Yasuni ^b   Gersnwin, Mevvill Eric ^a,d  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b KANAZAWA UNIVERSITY   (Japan)

^c MONASH UNIVERSITY   (Australia)

^d UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EPITOPE; MESSENGER RNA; MONOCLONAL ANTIBODY; NUCLEIC ACID; PYRUVATE DEHYDROGENASE COMPLEX;

ARTICLE; BILE DUCT; CLINICAL ARTICLE; CONTROLLED STUDY; GENE EXPRESSION; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; LIVER BIOPSY; LIVER CELL; MONONUCLEAR CELL; NUCLEIC ACID HYBRIDIZATION; PRIMARY BILIARY CIRRHOSIS; PRIORITY JOURNAL;

EID: 0031032692     PISSN: 02709139     EISSN: None     Source Type: Journal    
DOI: 10.1002/hep.510250106     Document Type: Article

References (32)

1. Scheuer PJ. Liver biopsy interpretation. London: Bailliere Tindall, 1980.
2. Rubin E, Schaffner F, Popper H. Primary biliary cirrhosis. Am J Pathol 1965;46:387-407.
3. Kaplan MM, Gandolfo JV, Quaroni EG. An enzyme-linked immunosorbent assay (ELISA) for detecting antimitochondrial antibody. HEPATOLOGY 1984;4:727-730.
4. Berg PA, Muscatello U, Horne RW, Roitt IM, Doniach D. Mitochondrial antibodies in primary biliary cirrhosis. II. The complement fixing antigen as a component of mitochondrial inner membranes. Br J Experiment Pathol 1969;50:200-208.
5. Baum H, Berg PA. The complex nature of mitochondrial antibodies and their relation to primary biliary cirrhosis [Review]. Semin Liver Dis 1981; 1:309-321.
6. Van de Water J, Gershwin ME, Leung P, Ansari A, Coppel RL. The autoepitope of the 74-kD mitochondrial autoantigen of primary biliary cirrhosis corresponds to the functional site of dihydrolipoamide acetyltransferase. J Exp Med 1988;167:1791-1799.
7. Fussey SP, Guest JR, James OF, Bassendine MF, Yeaman SJ. Identification and analysis of the major M2 autoantigens in primary biliary cirrhosis. Proc Natl Acad Sci U S A 1988;85:8654-8658.
8. Van de Water J, Fregeau D, Davis P, Ansari A, Danner D, Leung P, Coppel R, et al. Autoantibodies of primary biliary cirrhosis recognize dihydrolipoamide acetyltransferase and inhibit enzyme function. J Immunol 1988; 141:2321-2324.
9. Surh CD, Danner DJ, Ahmed A, Coppel RL, Mackay IR, Dickson ER, Gershwin ME. Reactivity of primary biliary cirrhosis sera with a human fetal liver cDNA clone of branched-chain alpha-keto acid dehydrogenase dihydrolipoamide acyltransferase, the 52 kD mitochondrial autoantigen. HEPATOLOGY 1989;9:63-68.
10. Fregeau DR, Davis PA, Danner DJ, Ansari A, Coppel RL, Dickson ER, Gershwin ME. Antimitochondrial antibodies of primary biliary cirrhosis recognize dihydrolipoamide acyltransferase and inhibit enzyme function of the branched chain alpha-ketoacid dehydrogenase complex. J Immunol 1989;142:3815-3820.
11. Fussey SP, Ali ST, Guest JR, James OF, Bassendine MF, Yeaman SJ. Reactivity of primary biliary cirrhosis sera with Escherichia coli dihydrolipoamide acetyltransferase (E2p): characterization of the main immunogenic region. Proc Natl Acad Sci U S A 1990;87:3987-3991.
12. Gershwin ME, Mackay IR, Sturgess A, Coppel RL. Identification and specificity of a cDNA encoding the 70 kd mitochondrial antigen recognized in primary biliary cirrhosis. J Immunol 1987;138:3525-3531.
13. Joplin R, Lindsay JG, Hubscher SG, Johnson GD, Shaw JC, Strain AJ, Neuberger JM. Distribution of dihydrolipoamide acetyltransferase (E2) in the liver and portal lymph nodes of patients with primary biliary cirrhosis: an immunohistochemical study. HEPATOLOGY 1991;14:442-447.
14. Joplin RE, Johnson GD, Matthews JB, Hamburger J, Lindsay JG, Hubscher SG, Strain AJ, et al. Distribution of pyruvate dehydrogenase dihydrolipoamide acetyltransferase (PDC-E2) and another mitochondrial marker in salivary gland and biliary epithelium from patients with primary biliary cirrhosis. HEPATOLOGY 1994;19:1375-1380.
15. Nakanuma Y, Tsuneyama K, Kono N, Hoso M, Van de Water J, Gershwin ME. Biliary epithelial expression of pyruvate dehydrogenase complex in primary biliary cirrhosis: an immunohistochemical and immunoelectron microscopic study. Hum Pathol 1995;26:92-98.
16. Tsuneyama K, Van de Water J, Leung PSC, Cha S, Nakanuma Y, Kaplan M, De Lellis R, et al. Abnormal expression of the E2 component of the pyruvate dehydrogenase complex on the luminal surface of biliary epithelium occurs before major histocompatibility complex class II and BB1/B7 expression. HEPATOLOGY 1995;21:1031-1037.
17. Joplin R, Lindsay JG, Johnson GD, Strain A, Neuberger J. Membrane dihydrolipoamide acetyltransferase (E2) on human biliary epithelial cells in primary biliary cirrhosis. Lancet 1992;339:93-94.
18. Van de Water J, Turchany J, Leung PS, Lake J, Munoz S, Surh CD, Coppel R, et al. Molecular mimicry in primary biliary cirrhosis. Evidence for biliary epithelial expression of a molecule cross-reactive with pyruvate dehydrogenase complex-E2. J Clin Invest 1993;91:2653-2664.
19. Nakanuma Y, Ohta G. Histometric and serial section observations of the intrahepatic bile ducts in primary biliary cirrhosis. Gastroenterology 1979; 76:1326-1332.
20. Chomezyneski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987;162:156-159.
21. Coppel RL, McNeilage LJ, Surh CD, Van de Water J, Spithill TW, Whittingham S, Gershwin ME. Primary structure of the human M2 mitochondrial autoantigen of primary biliary cirrhosis: dihydrolipoamide acetyltransferase. Proc Natl Acad Sci U S A 1988;85:7317-7321.
22. Surh CD, Coppel R, Gershwin ME. Structural requirement for autoreactivity on human pyruvate dehydrogenase-E2, the major autoantigen of primary biliary cirrhosis. Implication for a conformational autoepitope. J Immunol 1990;144:3367-3374.
23. Stasiak PC, Lane EB. Sequence of cDNA coding for human keratin 19. Nucleic Acids Res 1987;15:10058.
24. Viaene AI, Baert JH. Expression of cytokine-mRNA in squamous cell carcinomas and balloon-cell formation of human esophageal epithelium. Histochem J 1995;27:69-78.
25. Moll R, Franke WW, Schiller DL, Geiger B, Krepler R. The catalog of human cytokines: patterns of expression in normal epithelia, tumors and cultured cells. Cell 1982;31:11-24.
26. Surh CD, Ahmed-Ansari A, Gershwin ME. Comparative epitope mapping of murine monoclonal and human autoantibodies to human PDC-E2, the major mitochondrial autoantigen of primary biliary cirrhosis. J Immunol 1990;144:2647-2652.
27. Van de Water J, Ansari AA, Surh CD, Coppel R, Roche T, Bonkovsky H, Kaplan M, et al. Evidence for the targeting by 2-oxo-dehydrogenase enzymes in the T cell response of primary biliary cirrhosis. J Immunol 1991; 146:89-94.
28. Hsu M, Raine L, Frager H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled (PAP) procedures. J Histochem Cytochem 1981;29:557-580.
29. Naka H, Akagi Y, Kawamura T, Nishi M, Kamimura T, Ichida E, Kojima T. Ultrastructural findings of biliary epithelial cells and hepatocytes in primary biliary cirrhosis. J Clin Electron Microscopy 1979;12:824-825.
30. Tobe K. Electron microscopy of liver lesions in primary biliary cirrhosis. II. A bile duct with chronic non-suppurative destructive cholangitis. Acta Pathologica Japonica 1982;32:345-357.
31. Bernuau D, Feldmann G, Degott C, Gisselbrecht C. Ultrastructural lesions of bile ducts in primary biliary cirrhosis. A comparison with the lesions observed in graft versus host disease. Hum Pathol 1981;12:782-793.
32. Van de Water J, Gerson LB, Ferrell LD, Lake JR, Coppel RL, Batts KP, Wiesner RH, et al. Immunohistochemical evidence of disease recurrence following liver transplantation for primary biliary cirrhosis. HEPATOLOGY 1996;22:798-801.