The majority of N-methyl-D-aspartate receptor complexes in adult rat cerebral cortex contain at least three different subunits (NR1/NR2A/NR2B)

Molecular Pharmacology

Volumn 51, Issue 1, 1997, Pages 79-86

  Luo, Jianhong ^a,b   Wang, Yuehua ^a   Yasuda, Robert P ^a   Dunah, Anthone W ^a   Wolfe, Barry B ^a  

^a GEORGETOWN UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b Znejiang Medical University   (China)

Author keywords

[No Author keywords available]

Indexed keywords

AMPA RECEPTOR; GLUTAMATE RECEPTOR; MONOCLONAL ANTIBODY; N METHYL DEXTRO ASPARTIC ACID RECEPTOR; POLYCLONAL ANTIBODY; RECEPTOR SUBUNIT;

ANIMAL TISSUE; ANTIGEN RECOGNITION; ARTICLE; BRAIN CORTEX; CONTROLLED STUDY; EMBRYO; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; MALE; NONHUMAN; PRIORITY JOURNAL; RAT; RECEPTOR DENSITY; RECEPTOR SOLUBILIZATION;

EID: 0031032658     PISSN: 0026895X     EISSN: None     Source Type: Journal    
DOI: 10.1124/mol.51.1.79     Document Type: Article

References (38)

1. Monaghan, D. T., R. J. Bridges, and C. W. Cotman. The excitatory amino acid receptors: their classes, pharmacology, and distinct properties in the function of the central nervous system. Annu. Rev. Pharmacol. Toxicol. 29:365-402 (1989).
2. Collingridge, G. L., and W. Singer. Excitatory amino acid receptors and synaptic plasticity. Trends Pharmacol. Sci. 11:290-296 (1990).
3. Meldrum, B., and J. Garthwaite. Excitatory amino acid neurotoxicity and degenerative disease. Trends Pharmacol. Sci. 11:379-387 (1990).
4. Moriyoshi, K., M. Masu, T. Ishii, R. Shigemoto, N. Mizuno, and S. Nakanishi. Molecular cloning and characterization of the rat NMDA receptor. Nature (Lond.) 354:31-37 (1991).
5. Sugihara, H., K. Moriyoshi, T. Ishii, M. Masu, and S. Nakanishi. Structures and properties of seven isoforms of the NMDA receptor generated by alternative splicing. Biochem. Biophys. Res. Commun. 185:826-832 (1992).
6. Yamazaki, M., H. Mori, K. Araki, K. J. Mori, and M. Mishina. Cloning, expression and modulation of a mouse NMDA receptor subunit. FEBS Lett. 300:39-45 (1992).
7. Monyer, H., R. Sprengel, R. Schoepfer, A. Herb, M. Higuchi, H. Lomeli, N. Burnashev, B. Sakmann, and P. H. Seeburg. Heteromeric NMDA receptors: molecular and functional distinction of subtypes. Science (Washington D. C.), 256:1217-1221 (1992).
8. Meguro, H., H. Mori, K Araki, E. Kushiya, T. Kutsuwada, M. Yamazaki, T. Kumanishi, T. Arakawa, K. Sakimura, and M. Mishina. Functional characterization of a heteromeric NMDA receptor channel expressed from cloned cDNAs. Nature (Lond.) 357:70-74 (1992).
9. Kutsuwada, T., N. Kashiwabuchi, H. Mori, K. Sakimura, E. Kushiya, K. Araki, H. Meguro, H. Masaki, T. Kumanishi, M. Arakawa, and M. Mishina. Molecular diversity of the NMDA receptor channel. Nature (Lond.) 358:36-41 (1992).
10. Ishii, T., K. Moriyoshi, H. Sukihara, K. Kakurada, H. Katodani, M. Yokoi, C. Akazawa, R. Shigemoto, N. Mizuno, M. Mazu, and S. Nakanishi. Molecular characterization of the family of the N-methyl-D-aspartate receptor subunits. J. Biol. Chem. 268:2836-2843 (1993).
11. Laurie, D. J., and P. H. Seeburg. Ligand affinities at recombinant N-methyl-D-aspartate receptors depend on subunit composition. Eur. J. Pharmacol. 268:335-345 (1994).
12. Monyer, H., N. Burnashev, D. J. Laurie, B. Sakmann, and P. H. Seeburg. Developmental and regional expression in the rat brain and functional properties of four NMDA receptors. Neuron 12:529-540 (1994).
13. Beaton, J. A., K. Stemsrud, and D. T. Monaghan. Identification of a novel N-methyl-D-aspartate receptor population in the rat medial thalamus. J. Neurochem. 59:754-757 (1992).
14. Cunningham, M. D., and E. K. Michaelis. Solubilization and partial purification of 3-((+)-2-carboxypiperizine-4-yl)-[1,2-3H]propyl-1-phosphonic acid recognition proteins from rat brain synaptic membranes. J. Biol. Chem. 265:7768-7778 (1990).
15. 3H]MK801 binding in rat brain. J. Neurochem. 60:1344-1353 (1993).
16. Buller, A. L., H. C. Larson, B. E. Schneider, J. A. Beato, R. A. Morrisett, and D. T. Monaghan. The molecular basis of NMDA receptor subtypes: native receptor diversity is predicted by subunit composition. J. Neurosci. 14:5471-5484 (1994).
17. Stern, P., P. Behe, R. Schoepfer, and D. Colquhoun. Single-channel conductances of NMDA receptors expressed from cloned cDNAs: comparison with native receptors. Proc. R. Soc. Lond. Ser. B 250:271-277 (1992).
18. Brose, N., G. W. Huntley, Y. Stern-Bach, G. Sharma, J. H. Morrison, and S. F. Heinemann. Differential assembly of coexpressed glutamate receptor subunits in neurons of rat cerebral cortex. J. Biol. Chem. 269:16780-16784 (1994).
19. Sheng, M., J. Cummings, L. A. Roldan, Y. N. Jan, and L. Y. Jan. Changing subunit composition of heteromeric NMDA receptors during development of rat cortex. Nature (Lond.) 368:144-147 (1994).
20. Wang, Y., T. Z. Bosy, R. P. Yasuda, D. R. Grayson, S. Vicini, T. Pizzorusso, and B. B. Wolfe. Characterization of NMDA receptor subunit-specific antibodies: distribution of NR2A and NR2B receptor subunits in rat brain and ontogenic profile in the cerebellum. J. Neurochem. 65:176-183 (1995).
21. Wenthold, R. J., N. Yokotani, K. Doi, and K. Wada. Immunochemical characterization of the non-NMDA glutamate receptor using subunit-specific antibodies. J. Biol. Chem. 267:501-507 (1992).
22. Janknecht, R., G. de Martynoff, J. Lou, R. A. Hipskind, A. Nordheim, and H. G. Stunnenburg. Rapid and efficient purification of native histidinetagged protein expressed by recombinant vaccinia virus. Proc. Natl. Acad. Sci. USA 88:8972-8976 (1991).
23. Harlow, and D. Lane. Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1988).
24. Kohler, G., and C. Milstein. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature (Lond.) 256:495-497 (1975).
25. Luo, J., and B. B. Wolfe. The use of insoluble fusion proteins to purify antibodies. Biotechniques 19:544-550 (1995).
26. Chen, C., and H. Okayama. High-efficiency transformation of mammalian cells by plasmid DNA. Mol. Cell Biol. 7:2745-2751 (1987).
27. Towbin, H., T. Staehelin, and J. Gordon. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350-4354 (1979).
28. Luo, J., T. Z. Bosy, Y. Wang, R. P. Yasuda, and B. B. Wolfe. Ontogeny of NMDA R1 subunit protein expression in five regions of rat brain. Dev. Brain Res. 92:10-17 (1996).
29. Hollmann, M., J. Boulter, C. Maron, L. Beasley, J. Sullivan, G. Pecht, and S. Heinemann. Zinc potentiates agonist-induced currents at certain splice variants of NMDA receptor. Neuron 10:943-954 (1993).
30. Petralia, R. S., N. Yokotani, and R. R. Wenthold. Light and electron microscope distribution of the NMDA receptor subunit NMDA R1 in the rat nervous system using a selective anti-peptide antibody. J. Neurosci. 14:667-696 (1994).
31. Brose, N., G. P., Gasic, D. E. Vetter, J. M. Sullivan, and S. F. Heinemann. Protein chemical characterization and immunocytochemical localization of the NMDA receptor subunit NMDA R1. J. Biol. Chem. 268:22663-22671 (1993).
32. Dunah, A. W., R. P. Yasuda, Y. H. Wang, J. Luo, M. Davila-Garcia, M. Gbadegesin, S. Vicini, and B. B. Wolfe. Regional and ontogenic expression of the N-methyl-D-aspartate receptor subunit NR2D protein in the rat brain using a subunit-specific antibody. J. Neurochem. 67:2335-2345 (1996).
33. Vicini, S., J. F. Wang, J. S. Poling, and D. R. Grayson. Functional and pharmacological differences between recombinant and native NMDA receptors. Soc. Neurosci. Abstr. 21:86 (1995).
34. Li, J. H., J. F. Wang, J. H. Luo, Y. H. Wang, B. B. Wolfe, and S. Vicini. Properties of NMDA responses in HEK293 cells expressing heteromers of NR1/NR2A/NR2B subunits. Soc. Neurosci. Abstr. 22:592 (1996).
35. Chazot, P. L., S. K. Coleman, M. Cik, and F. A. Stephenson. Molecular characterization of N-methyl-D-aspartate receptors expressed in mammalian cells yields evidence for the coexistence of three subunit types within a discrete receptor molecule. J. Biol. Chem. 269:24403-24409 (1994).
36. Didier M., M. Xu, S. A. Berman, and S. Bursztajn. Differential expression and co-assembly of NMDAζ1 and € subunits in the mouse cerebellum during postnatal development. Neuroreport 6:2255-2259 (1995).
37. Williams, K., S. L. Russell, Y. M. Shen, and P. B. Molinoff. Developmental switch in the expression of NMDA receptors occurs in vivo and in vitro. Neuron 10:267-278 (1993).
38. Williams, K. Ifenprodil discriminates subtypes of the N-methyl-D-aspartate receptor: selectivity and mechanisms at recombinant heteromeric receptors. Mol. Pharmacol. 44:851-859 (1993).