Use of a multiwell fluorescence scanner with propidium iodide to assess NMDA mediated excitotoxicity in rat cortical neuronal cultures

Neuroscience Letters

Volumn 221, Issue 2-3, 1997, Pages 149-152

  Rudolph, Joseph G ^a   Lemasters, John J ^b   Crews, Fulton T ^a,b  

^a UNIVERSITY OF FLORIDA   (United States)

^b UNIVERSITY OF NORTH CAROLINA   (United States)

Author keywords

excitotoxicity; glutamate; N methyl D aspartate; neurons; propidium iodide

Indexed keywords

ALCOHOL; DIZOCILPINE; EXCITOTOXIN; IFENPRODIL; LACTATE DEHYDROGENASE; N METHYL DEXTRO ASPARTIC ACID; N METHYL DEXTRO ASPARTIC ACID RECEPTOR BLOCKING AGENT;

ANIMAL CELL; ARTICLE; BRAIN CORTEX; CELL CULTURE; CELL DEATH; CONTROLLED STUDY; FLUORESCENCE ANALYSIS; NONHUMAN; PRIORITY JOURNAL; RAT;

ANIMALS; CELL SURVIVAL; CELLS, CULTURED; CEREBRAL CORTEX; EXCITATORY AMINO ACID AGONISTS; L-LACTATE DEHYDROGENASE; N-METHYLASPARTATE; NEURONS; PROPIDIUM; RATS;

EID: 0031032299     PISSN: 03043940     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0304-3940(96)13313-9     Document Type: Article

References (11)

1. Aoki, C., Kaneko, T., Starr, A. and Pickel, V.M., Identification of mitochondrial and non-mitochondrial glutaminase within select neurons and glia of rat forebrain by electron microscopic immunocytochemistry, J. Neurosci. Res., 28 (1991) 531-548.
2. Chandler, L.J., Sumners, C. and Crews, F.T., Chronic ethanol exposure potentiates NMDA excitotoxicity in cerebral cortical neurons, J. Neurochem., 60 (1993) 1578-1581.
3. Chandler, L.J., Sumners, C. and Crews, F.T., Ethanol inhibits NMDA receptor-mediated excitotoxicity in rat primary neuronal cultures, Alcohol. Clin. Exp. Res., 17 (1993) 54-60.
4. Hogstead, S., Svenneby, G., Torgner, I.A., Kvamme, E., Hertz, L. and Schousboe, A., Glutaminase in neurons and astrocytes cultured from mouse brain: kinetic properties and effects of phosphate, glutamate, and ammonia, Neurochem. Res., 13 (1988) 383-388.
5. Koh, J.Y. and Choi, D.W., Vulnerability of cultured cortical neurons to damage by excitotoxins: differential susceptibility of neurons containing NADPH-diaphorase, J. Neurosci., 8 (1988) 2153-2163.
6. Micheals, R.L. and Rothman, S.M., Antagonist pharmacology and intracellular calcium concentrations, J. Neurosci., 10 (1990) 283-292.
7. Nieminen, A.L., Gores, G.J., Bond, J.M., Inberti, R., Herman, B. and Lemasters, J.J., A novel cytotoxicity assay using a multiwell fluorescence scanner, Toxicol. Appl. Pharmacol., 115 (1992) 147-155.
8. Popik, P., Layer, R.T., Fossom, L.H., Benveniste, M., Geter-Douglass, B., Witkin, J.M. and Skolnick, P., NMDA antagonist properties of the putative antiaddictive drug, ibogaine, J. Pharmacol. Exp. Ther., 275 (1995) 753-760.
9. Tamura, Y., Sato, Y., Yokota, T., Akaike, A., Sasa, M. and Takori, S., Ifenprodil prevents glutamate cytotoxicity via polyamine modulatory sites of N-methyl-D-aspartate receptors in cultured cortical neurons, J. Pharmacol. Exp. Ther., 2 (1993) 1017-1025.
10. Trost, L.C. and Lemasters, J.J., A cytotoxicity assay for tumor necrosis factor employing a multiwell fluorescence scanner, Anal. Biochem., 220 (1994) 149-153.
11. Wrobleski, F. and LaDue, J.S., Lactic dehydrogenase activity in blood, Proc. Soc. Exp. Biol. Med., 90 (1955) 210-213.