Morphological and biochemical analyses on fibroblasts and self-produced collagens in a novel three-dimensional culture

British Journal of Dermatology

Volumn 136, Issue 1, 1997, Pages 6-11

  Ishikawa, O ^a   Kondo, A ^a   Okada, K ^a   Miyachi, Y ^a   Furumura, M ^b  

^a GUNMA UNIVERSITY   (Japan)

^b KYUSHU UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ASCORBIC ACID; HYDROXYPROLINE;

ARTICLE; CELL CULTURE; COLLAGEN METABOLISM; CONFOCAL LASER MICROSCOPY; DERMIS; EXTRACELLULAR MATRIX; FIBROBLAST; HUMAN; MORPHOLOGY; PRIORITY JOURNAL;

EID: 0031031187     PISSN: 00070963     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2133.1997.tb08738.x     Document Type: Article

References (19)

1. Liotta LA, Vembu D, Kleinman HK et al. Collagen required for proliferation of cultured connective tissue cells but not their transformed counterparts. Nature 1978; 272: 622-4.
2. Gallagher JT, Gasiunas N, Schor SL. Synthesis of glycosaminoglycans by human skin fibroblasts cultured in collagen gels. Biochem J 1980; 190: 243-54.
3. Sarbar R, Hull B, Merrill C et al. Regulation of proliferation of fibroblasts of low and high population doubling levels grown in collagen lattice. Mech Age Dev 1981; 17: 107-17.
4. Kleinman HK, Klebe RJ, Martin GR. Role of collagenous matrices in the adhesion and growth of cells. J Cell Biol 1981; 88: 473-85.
5. Gibson GI, Schor SL, Grant ME. Effects of matrix macromolecules on chondrocytes gene expression: synthesis of a low molecular weight collagen species by cells cultured within collagen gels. J Cell Biol 1982; 93: 767-74.
6. Hata R, Hori H, Nagai Y et al. Selective inhibition of type I collagen synthesis in osteoblastic cells by epidermal growth factor. Endocrinology 1984; 115: 867-76.
7. Hata R, Sunada H, Arai K et al. Regulation of collagen metabolism and growth by epidermal growth factor and ascorbate in cultured human skin fibroblasts. Eur J Biochem 1988; 173: 261-7.
8. Hata R, Senoo H. L-Ascorbic acid 2-phosphate stimulates collagen accumulation, cell proliferation, and formation of a three-dimensional tissue-like substance by skin fibroblasts. J Cell Physiol 1989; 138: 8-16.
9. Ishikawa O, Yokoyama Y, Miyachi Y. Disaccharide analysis of dermal fibroblast-derived glycosaminoglycans in the three-dimensional culture. J Dermatol Sci 1994; 8: 203-7.
10. Poole CA, Brookes MH, Clover GM. Keratinocyte networks visualized in the living cornea using vital dyes. J Cell Sci 1993; 106: 6855-91.
11. Piez KA, Eigner EA, Lewis MS. The chromatographic separation and amino acid composition of the subunits of several collagens. Biochemistry 1963; 2: 58-65.
12. Einarsson S. Selective determination of secondary amino acids using precolumn derivatization with 9-fluorenylmethylchloroformate and reversed-phase high-performance liquid chromatograpy. J Chromatogr 1985; 348: 213-20.
13. Lyons BL, Schwarz RI. Ascorbate stimulation of PAT cells causes an increase in transcription rates and a decrease in degradation rates of procollagen mRNA. Nucleic Acids Res 1984; 12: 2569-79.
14. Tajima S, Pinnell SR. Regulation of collagen synthesis by ascorbic acid. Ascorbic acid increases type I collagen mRNA. Biochem Biophijs Res Commun 1982; 106: 632-7.
15. Schwarz E, Bienkowski RS, Colotoff-Schiller B et al. Changes in the components of extracellular matrix and in growth properties of cultured aortic smooth muscle cells upon ascorbate feeding. J Cell Biol 1982; 92: 462-70.
16. Peterkofsky B. The effect of ascorbic acid on collagen polypeptide synthesis and proline hydroxylation during the growth of cultured fibroblasts. Arch Biochem Biophys 1982; 152: 318-28.
17. Grinnell F, Fukamizu H, Pawelek P et al. Collagen processing, and fibril bundle assembly in matrix produced by fibroblasts in long-term culture supplemented with ascorbic acid. Exp Cell Res 1989; 181: 483-91.
18. Bell E, Ivarsson B, Merrill C. Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro. Proc Natl Acad Sci USA 1979; 76: 1274-8.
19. Mauch C, Hatamochi A, Scharffetter K et al. Down regulation of collagen synthesis in fibroblasts within three-dimensional collagen lattices involves transcriptional and posttranscriptional mechanisms. Exp Cell Res 1988; 178: 1493-503.