Phosphorothioate oligonucleotides: Largely reduced (n-1)-mer and phosphodiester content through the use of dimeric phosphoramidite synthons

Bioorganic and Medicinal Chemistry Letters

Volumn 7, Issue 1, 1997, Pages 73-78

  Krotz, Achim H ^a   Klopchin, Patrick ^a   Cole, Douglas L ^a   Ravikumar, Vasulinga T ^a  

^a ISIS PHARMACEUTICALS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

OLIGODEOXYNUCLEOTIDE PHOSPHOROTHIOATE; OLIGONUCLEOTIDE;

ARTICLE; DRUG IMPURITY; DRUG SYNTHESIS; GAS CHROMATOGRAPHY; INTERMETHOD COMPARISON; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY;

EID: 0031031114     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(96)00573-2     Document Type: Article

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29. 3CN. Coupling time 200 s, sulfurization time 900 s (not optimized).
30. 18 = 93%.
31. Precise quantitation below 1% is difficult due to lack of baseline resolution. The actual value may be significantly lower.
32. 19) 22-24 min.
33. 5b