Identification of cytoplasmic subdomains that control pH-sensing of the Na+/H+ exchanger (NHE1): pH-maintenance, ATP-sensitive, and flexible loop domains

Journal of Biochemistry

Volumn 121, Issue 2, 1997, Pages 295-303

  Ikeda, Toshitaro ^a   Schmitt, Bernhard ^a   Pouysségur, Jacques ^b   Wakabayashi, Shigeo ^a   Shigekawa, Munekazu ^a  

^a NATIONAL CEREBRAL AND CARDIOVASCULAR CENTER   (Japan)

^b CENTRE DE BIOCHIMIE   (France)

Author keywords

ATP depletion; Domain structure; Growth factor

Indexed keywords

5 (N ETHYL N ISOPROPYL)AMILORIDE; ADENOSINE TRIPHOSPHATE; CALCIUM ION; CALMODULIN; GROWTH FACTOR; IONOMYCIN; SODIUM ION; THROMBIN;

ANIMAL CELL; ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CELL LINE; CELL PH; CONTROLLED STUDY; DELETION MUTANT; NONHUMAN; PROTEIN DOMAIN; PROTEIN INTERACTION; PROTON SODIUM EXCHANGE; SODIUM TRANSPORT;

ANIMALIA;

EID: 0031029728     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021586     Document Type: Article

References (34)

1. + antiporter. Cell 56, 271-280
2. + exchanger isoform (NHE-3). J. Biol. Chem. 267, 9340-9346
3. Orlowaki, J., Kandasamy, R.A., and Shull, G.E. (1992) Molecular cloning of putative members of the Na/H exchanger gene family. cDNA cloning, deduced amino acid sequence, and mRNA tissue expression of the rat Na/H exchanger NHE-1 and two structurally related proteins. J. Biol. Chem. 267, 9331-9339
4. + exchanger isoform (NHE-2). J. Biol. Chem. 268, 11917-11924
5. Wang, Z., Orlowski, J., and Shull, G.E. (1993) Primary structure and functional expression of a novel gastrointestinal isoform of the rat Na/H exchanger. J. Biol. Chem. 268, 11925-11928
6. + exchange and growth factor-induced cytosolic pH changes. Role in cellular proliferation. Biochim. Biophys. Acta 988, 73-97
7. + exchange system: a genetic approach. Trends Biochem. Sci. 10, 453-455
8. + antiporter, a glycoprotein of 110 kD. Science 247, 723-726
9. +-sensing." Proc. Natl. Acad. Sci. USA 89, 2424-2428
10. + exchanger gene (NHE1) and amplification of the mutated allele confer amiloride resistance upon chronic acidosis. Proc. Natl. Acad. Sci. USA 90, 4508-4512
11. + exchanger isoform 1 (NHE1). Evidence for an additional mechanism not requiring direct phosphorylation. J. Biol. Chem. 269, 5583-5588
12. + exchanger NHE1 possesses N- and O-linked glycosylation restricted to the first N-terminal extracellular domain. Biochemistry 33, 10463-10469
13. + exchanger isoforms NHE1 and NHE3 exist as stable dimers in membranes with a high degree of specificity for homodimers. J. Biol. Chem. 269, 2589-2596
14. + exchanger isoform 1 (NHE1) is a novel member of the calmodulin-binding proteins. J. Biol. Chem. 269, 13703-13709
15. 2+ regulation-defective. J. Biol. Chem. 269, 13710-13715
16. 2+ responsiveness to the apical isoform NHE3. J. Biol. Chem. 270, 26460-26465
17. +. J. Biol. Chem. 259, 10989-10994
18. + exchange and cytoplasmic pH in the action of growth factors in human fibroblasts. Nature 304, 645-648
19. + exchange in rat thymic lymphocytes. J. Gen. Physiol. 85, 765-787
20. + exchangers stably expressed in a fibroblast cell line. J. Biol. Chem. 268, 25527-25535
21. + exchanger (NHE3): Resistance to amiloride and inhibition by protein kinase C. Proc. Natl. Acad. Sci. USA 90, 9110-9114
22. + antiport in cultured human cells. J. Biol. Chem. 261, 5460-5466
23. + antiporter. Analysis of phosphorylation and aubcellular localization. J. Biol. Chem. 269, 8741-8748
24. + exchanger stably expressed in Chinese hamster ovary cells. ATP dependence, osmotic sensitivity, and role in cell proliferation. J. Biol. Chem. 269, 23544-23552
25. +H exchanger in renal microvillus membrane vesicles. Nature 299, 161-163
26. + antiport activity in hamster fibroblasts precludes growth at neutral and acidic pH. Proc. Natl. Acad. Sci. USA 81, 4833-4837
27. + antiporter. J. Biol. Chem. 259, 5809-5815
28. + antiport to osmotic stress. Am. J. Physiol. 269, C998-C1007
29. Chou, P.Y. and Fasman, G.D. (1974) Conformational parameters for amino acids in helical, beta-sheet, and random coil regions calculated from proteins. Biochemistry 13, 211-222
30. + exchanger NHE3 isoform. J. Biol. Chem. 271, 3590-3599
31. + antiport. J. Biol. Chem. 266, 15406-15413
32. + exchanger interacts with mammalian heat shock protein. Biochemistry 34, 10412-10420
33. + exchanger isoform NHE3 are involved in stimulation and inhibition by protein kinases/growth factors. J. Biol. Chem. 270, 13716-13725
34. Orlowski, J. (1993) Heterologous expression and functional properties of amiloride high affinity (NHE-1) and low affinity (NHE-3) isoforms of the rat Na/H exchanger. J. Biol. Chem. 268, 16369-16377