Characterization of recombinant perlecan domain I and its substitution by glycosaminoglycan and oligosaccharides

European Journal of Biochemistry

Volumn 243, Issue 1-2, 1997, Pages 115-121

  Costell, Mercedes ^a   Mann, Karlheinz ^a   Yamada, Yoshihiko ^b   Timpl, Rupert ^a  

^a MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

^b NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

Basement membrane; Protein module; Proteoglycan; Recombinant production

Indexed keywords

CHONDROITIN SULFATE; DERMATAN SULFATE; GLYCOSAMINOGLYCAN; HEPARAN SULFATE; MEMBRANE PROTEIN; PERLECAN; PROTEOGLYCAN; RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; BINDING SITE; CARBOHYDRATE ANALYSIS; CELL CULTURE; CIRCULAR DICHROISM; CONTROLLED STUDY; GENE TRANSFER; KIDNEY CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN MODIFICATION; PROTEIN PURIFICATION; SEQUENCE ANALYSIS;

EID: 0031029467     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.t01-1-00115.x     Document Type: Article

References (35)

1. Aviezer, D., Hecht, D., Safran, M., Eisinger, M., David, G. & Yayon, A. (1994) Perlecan, basal lamina proteoglycan, promotes basic fibroblast growth factor-receptor binding, mitogenesis, and angiogenesis, Cell 79, 1005-1013.
2. Battaglia, C., Mayer, U., Aumailley, M. & Timpl, R. (1992) Basement membrane heparan sulfate proteoglycan binds to laminin by its heparan sulfate chains and to nidogen by sites in the protein core, Eur. J. Biochem. 208, 359-366.
3. Battaglia, C., Aumailley, M., Mann, K., Mayer, U. & Timpl, R. (1993) Structural basis of β1 integrin-mediated cell adhesion to a large heparan sulphate proteoglycan from basement membranes, Eur. J. Cell Biol. 61, 92-99.
4. Bitter, T. & Muir, H. (1962) A modified uronic acid carbazole reaction, Anal. Biochem. 4, 330-334.
5. Bork, P. & Patthy, L. (1995) The SEA module: a new extracellular domain associated with O-glycosylation, Protein Sci. 4, 1421-1425.
6. Bourdon, M. A. (1990) Structure and role of cloned small proteoglycans, in Extracellular matrix genes (Sandell, L. & Boyd, C., eds) pp. 157-174, Academic Press, New York.
7. Bourdon, M. A., Krusius, T., Campbell, S., Schwartz, N. B. & Ruoslahti, E. (1987) Identification and synthesis of a recognition signal for the attachment of glycosaminoglycans to proteins, Proc. Natl Acad. Sci. USA 84, 3194-3198.
8. Chakravarti, S., Horchar, T., Jefferson, B., Laurie, G. W. & Hassell, J. R. (1995) Recombinant domain III of perlecan promotes cell attachment through its RGDS sequence, J. Biol. Chem. 270, 404-409.
9. Danielson, K. G., Martinez-Hernandez, A., Hassell, J. R. & Iozzo, R. V. (1992) Establishment of a cell line from the EHS tumor: biosynthesis of basement membrane constituents and characterization of a hybrid proteoglycan containing heparan and chondroitin sulfate chains, Matrix 12, 22-35.
10. Gooley, A. A., Classon, B. J., Marschalek, R. & Williams, K. L. (1991) Glycosylation sites identified by detection of glycosylated amino acids released from Edman degradation: the identification of Xaa-Pro-Xaa-Xaa as a motif for Thr-O-glycosylation, Biochem. Biophys. Res. Commun. 178, 1194-1201.
11. Hardingham, T. E. & Fosang, A. J. (1992) Proteoglycans: many forms and many functions, FASEB J. 6, 861-870.
12. Hascall, V. C., Heinegard, D. K. & Wight, T. N. (1991) Proteoglycans: metabolism and pathology, in Cell biology of extracellular matrix, (Hay, E. D., ed.) 2nd edn, pp. 149-175, Plenum Press, New York.
13. Hassell, J. R., Leshon, W. C., Ledbetter, S. R., Tyree, B., Suzuki, S., Kato, M., Kimata, K. & Kleinman, H. K. (1985) Isolation of two forms of basement membrane proteoglycan, J. Biol. Chem. 260, 8098-8105.
14. Hayashi, K., Madri, J. A. & Yurchenco, P. D. (1992) Endothelial cells interact with the core protein of basement membrane perlecan through β1 and β3 integrins: an adhesion modulated by glycosaminoglycan, J. Cell Biol. 119, 945-959.
15. Heremans, A., Cassiman, J.-J., van den Berghe, H. & David, G. (1988) Heparan sulfate proteoglycan from the extracellular matrix of human lung fibroblasts. Isolation, purification, and core protein characterization, J. Biol. Chem. 263, 4731-4739.
16. Iozzo, R., Cohen, I. R., Grässel, S. & Murdoch, A. D. (1994) The biology of perlecan: the multifaceted heparan sulphate proteoglycan of basement membranes and pericellular matrices, Biochem. J. 302, 625-639.
17. Isemura, M., Sato, X., Yamaguchi, Y., Aikawa, J., Munakata, H., Hayashi, N., Yosizawa, Z., Nakamura, T., Kubota, A., Arakawa, M. & Hsu, C.-C. (1987) Isolation and characterization of fibronectin-binding proteoglycan carrying both heparan sulfate and dermatan sulfate chains from human placenta, J. Biol. Chem. 262, 8926-8933.
18. Kallunki, P. & Tryggvason, K. (1992) Human basement membrane heparan sulfate proteoglycan core protein: A 467-kD protein containing multiple domains resembling elements of the low density lipoprotein receptor, laminin, neural cell ahesion molecules and epidermal growth factor, J. Cell Biol. 116, 559-571.
19. Klein, G., Conzelmann, S., Beck, S., Timpl, R. & Müller, C. A. (1994) Perlecan in the human bone marrow: a growth factor presenting but repelling extracellular matrix component for hematopoietic cells, Matrix Biol. 14, 457-465.
20. Kokenyesi, R. & Silbert, J. E. (1995) Formation of heparan sulfate or chondroitin/dermatan sulfate on recombinant domain I of mouse perlecan expressed in chinese hamster ovary cells. Biochem. Biophys. Res. Commun. 211, 262-267.
21. Mayer, U., Nischt, R., Pöschl, E., Mann, K., Fukuda, K., Gerl, M., Yamada, Y. & Timpl, R. (1993) A single EGF-like motif of laminin is responsible for high affinity nidogen binding, EMBO J. 12, 1879-1885.
22. Murdoch, A. D., Dodge, G. R., Cohen, I., Tuan, R. S. & Iozzo, R. V. (1992) Primary structure of the human heparan sulfate proteoglycan from basement membrane (HSPG2/Perlecan). A chimeric molecule with multiple domains homologous to the low density lipoprotein receptor, laminin, neural cell adhesion molecules, and epidermal growth factor, J. Biol. Chem. 267, 8544-8557.
23. Nischt, R., Pottgiesser, J., Krieg, T., Mayer, U., Aumailley, M. & Timpl, R. (1991) Recombinant expression and properties of the human calcium-binding extracellular matrix protein BM-40, Eur. J. Biochem. 200, 529-536.
24. Noonan, D. M., Fulle, A., Valente, P., Cai, S., Horigan, E., Sasaki, M., Yamada, Y. & Hassell, J. R. (1991) The complete sequence of perlecan, a basement membrane heparan sulfate proteoglycan, reveals extensive similarity with laminin A chain, LDL-receptor and N-CAM, J. Biol. Chem. 266, 22939-22947.
25. Paulsson, M., Yurchenco, P. D., Ruben, G. C., Engel, J. & Timpl, R. (1987) Structure of low density heparan sulfate proteoglycan isolated from a mouse tumor basement membrane, J. Mol. Biol. 197, 297-313.
26. Provencher, S. W. & Glöckner, J. (1981) Estimation of globular protein secondary structure from circular dichroism, Biochemistry 20, 33-37.
27. Sasaki, T. Göhring, W., Pan, T.-C., Chu, M.-L. & Timpl, R. (1995) Binding of mouse and human fibulin-2 to extracellular matrix ligands, J. Mol. Biol. 254, 892-899.
28. Schulze, B., Mann, K., Battistutta, R., Wiedemann, H. & Timpl, R. (1995) Structural properties of recombinant domain III-3 of perlecan containing a globular domain inserted into an epidermal-growth-factor-like motif, Eur. J. Biochem. 231, 551-556.
29. Tillet, E., Wiedemann, H., Golbik, R., Pan, T.-C., Zhang, R.-Z., Mann, K., Chu, M.-L. & Timpl, R. (1994) Recombinant expression and structural and binding properties of α1(VI) and α2(VI) chains of human collagen type VI, Eur. J. Biochem. 221, 177-185.
30. Timpl, R. (1982) Antibodies to collagens and procollagens, Methods Enzymol. 82, 472-498.
31. Timpl, R. (1993) Proteoglycans of basement membranes, Experientia (Basel) 49, 417-428.
32. Tsen, G., Halfter, W., Kröger, S. & Cole, G. J. (1995) Agrin is a heparan sulfate proteoglycan, J. Biol. Chem. 270, 3392-3399.
33. Whitelock, J. M., Murdoch, A. D., Iozzo, R. V. & Underwood, P. A. (1996) The degradation of human endothelial cell-derived perlecan and release of bound basic fibroblast growth factor by stromelysin, collagenase, plasmin and heparanases, J. Biol. Chem. 271, 10079-10086.
34. Wilson, I. B. H., Gavel, Y. & von Heijne, G. (1991) Amino acid distributions around O-linked glycosylation sites, Biochem. J. 275, 529-534.
35. Zhang, L. & Esko, J. D. (1994) Amino acid determinants that drive heparan sulfate assembly in a proteoglycan, J. Biol. Chem. 269, 19295-19299.