Determinants of specificity for α-conotoxin MII on α3β2 neuronal nicotinic receptors

Molecular Pharmacology

Volumn 51, Issue 2, 1997, Pages 336-342

  Harvey, Scott C ^a   Mcintosh, J Michael ^b,c   Cartier, G Edward ^b   Maddox, Floyd N ^a   Luetje, Charles W ^a  

^a UNIVERSITY OF MIAMI MILLER SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF UTAH   (United States)

^c UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYLCHOLINE; ATROPINE; CHIMERIC PROTEIN; COLLAGENASE; CONOTOXIN; ISOLEUCINE; LYSINE; NICOTINIC RECEPTOR; RECEPTOR SUBTYPE; RECEPTOR SUBUNIT; THREONINE;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DRUG BINDING SITE; GENE EXPRESSION; NONHUMAN; OOCYTE; PRIORITY JOURNAL; RECEPTOR SENSITIVITY; SITE DIRECTED MUTAGENESIS; XENOPUS LAEVIS;

EID: 0031026798     PISSN: 0026895X     EISSN: None     Source Type: Journal    
DOI: 10.1124/mol.51.2.336     Document Type: Article

References (38)

1. Elgoyhen, A. B., D. S. Johnson, J. Boulter, D. E. Vetter, and S. Heinemann. α9: an acetylcholine receptor with novel pharmacological properties expressed in rat cochlear hair cells. Cell 79:705-715 (1994).
2. Sargent, P. B. The diversity of neuronal nicotinic acetylcholine receptors. Annu. Rev. Neurosci. 16:403-443 (1993).
3. Wada, K., M. Ballivet, J. Boulter, J. Connolly, E. Wada, E. S. Deneris, L. W. Swanson, S. Heinemann, and J. Patrick. Functional expression of a new pharmacological subtype of brain nicotinic acetylcholine receptor. Science (Washington D. C.) 240:330-334 (1988).
4. Luetje, C. W., and J. Patrick. Both α-and β-subunits contribute to the agonist sensitivity of neuronal nicotinic acetylcholine receptors. J. Neurosci. 11:837-845 (1991).
5. Luetje, C. W., K. Wada, S. Rogers, S. N. Abramson, K. Tsuji, S. Heinemann, and J. Patrick. Neurotoxins distinguish between different neuronal nicotinic acetylcholine receptor subunit combinations. J. Neurochem. 55: 632-640 (1990).
6. Duvoisin, R. M., E. S. Deneris, J. Boulter, J. Patrick, and S. Heinemann. The functional diversity of the neuronal nicotinic acetylcholine receptors is increased by a novel subunit: β4. Neuron 3:487-496 (1989).
7. Boulter, J., J. Connolly, E. Deneris, D. Goldman, S. Heinemann, and J. Patrick. Functional expression of two neuronal nicotinic acetylcholine receptors from cDNA clones identifies a gene family. Proc. Natl. Acad. Sci. USA 84:7763-7767 (1987).
8. Chiara, D. C., and J. B. Cohen. Identification of amino acids contributing to high and low affinity d-tubocurarine sites on the Torpedo nicotinic acetylcholine receptor subunits. Biophys. J. 61:A106 (1992).
9. Czajkowski, C., and A. Karlin. Agonist binding site of Torpedo electric tissue nicotinic acetylcholine receptor: a negatively charged region of the δ subunit within 0.9 nm of the α subunit binding site disulfide. J. Biol. Chem. 266:22603-22612 (1991).
10. 190 in the α-subunit of the nicotinic acetylcholine receptor. J. Biol. Chem. 264:12666-12672 (1989).
11. Czajkowski, C., C. Kaufmann, and A. Karlin. Negatively charged amino acid residues in the nicotinic receptor γ subunit that contribute to the binding of acetylcholine. Proc. Natl. Acad. Sci. USA 90:6285-6289 (1993).
12. Dennis, M., J. Giraudat, F. Kotzyba-Hibert, M. Goeldner, C. Hirth, J.-Y. Chang, C. Lazure, M. Chretien, and J.-P. Changeux. Amino acids of the Torpedo marmorata acetylcholine receptor α subunit labelled by photoaffinity ligand for acetylcholine binding site. Biochemistry 27:2346-2357 (1988).
13. Galzi, J., F. Revah, D. Black, M. Goeldner, C. Hirth, and J.-P. Changeux. Identification of a novel amino acid α-tyrosine 93 within the cholinergic ligands-binding sites of the acetylcholine receptor by photoaffinity labeling. J. Biol. Chem. 265:10430-10437 (1990).
14. Kao, P. N., A. J. Dwork, R.-R. J. Kaldany, M. S. Silver, J. Wideman, S. Stein, and A. Karlin. Identification of the α subunit half-cystine specifically labelled by an affinity reagent for the acetylcholine receptor binding site. J. Biol. Chem. 259:11662-11665 (1984).
15. 3H]nicotine as an agonist photoaffinity label. Biochemistry 30:6987-6997 (1991).
16. Pedersen, S. E., and J. B. Cohen. d-Tubocurarine binding sites are located at the α-γ and α-δ subunit interfaces of the nicotinic acetylcholine receptor. Proc. Natl. Acad. Sci. USA 87:2785-2789 (1990).
17. Luetje, C. W., M. Piattoni, and J. Patrick. Mapping of ligand binding sites of neuronal nicotinic acetylcholine receptors using chimeric α subunits. Mol. Pharmacol. 44:657-666 (1993).
18. Figl, A., B. N. Cohen, M. W. Quick, N. Davidson, and H. A. Lester. Regions of β4-β2 subunit chimeras that contribute to the agonist selectivity of neuronal nicotinic receptors. FEBS Lett. 308:245-248 (1992).
19. Cohen, B. N., A. Figl, M. W. Quick, C. Labarca, N. Davidson, and H. A. Lester. Regions of β2 and β4 responsible for differences between the steady state dose-response relationships of the α3β2 and α3β4 neuronal nicotinic receptors. J. Gen. Physiol. 105:745-764 (1995).
20. Harvey, S. C., and C. W. Luetje. Determinants of competitive antagonist sensitivity on neuronal nicotinic receptor β subunits. J. Neurosci. 16: 3798-3806 (1996).
21. Cartier, G. E., D. Yoshikami, W. R. Gray, S. Luo, B. M. Olivera, and J. M. McIntosh. A new α-conotoxin which targets α3β2 nicotinic acetylcholine receptors. J. Biol. Chem. 271:7522-7528 (1996).
22. Papke, R. L., R. M. Duvoisin, and S. Heinemann. The amino terminal half of the nicotinic beta-subunit extracellular domain regulates the kinetics of inhibition by neuronal bungarotoxin. Proc. R. Soc. Lond. 252:141-148 (1993).
23. Cartier, G. E., D. Yoshikami, S. Luo, B. M. Olivera, and J. M. McIntosh. α-Conotoxin-MII (α-CTx-MII) interaction with neuronal nicotinic acetylcholine receptors. Soc. Neurosci. Abstr. 22:268 (1996).
24. Higuchi, R. Recombinant PCR, in PCR Protocols: A Guide to Methods and Applications (M. A. Innis, D. H. Gelfand, J. J. Sninsky and T. J. White, eds.). Academic Press, San Diego (1990).
25. Kenakin, T. Pharmacologic Analysis of Drug-Receptor Interaction. Raven, New York, 323-343 (1993).
26. Sine, S., and P. Taylor. The relationship between agonist occupation and the permeability response of the cholinergic receptor revealed by bound cobra α-toxin. J. Biol. Chem. 255:10144-10156 (1980).
27. Fu, D. X., and S. M. Sine. Competitive antagonists bridge the α-γ subunit interface of the acetylcholine receptor through quaternary ammonium-aromatic interactions. J. Biol. Chem. 269:26152-26157 (1994).
28. Sine, S. M. Molecular dissection of subunit interfaces in the acetylcholine receptor: identification of residues that determine curare selectivity. Proc. Natl. Acad. Sci. USA 90:9436-9440 (1993).
29. Sine, S. M., H. J. Kreienkamp, N. Bren, R. Maeda, and P. Taylor. Molecular dissection of subunit interfaces in the acetylcholine receptor: identification of determinants of α-conotoxin M1 selectivity. Neuron 15:205-211 (1995).
30. Johnson, D. S., J. Martinez, A. B. Elgoyhen, S. F. Heinemann, and J. M. McIntosh. α-Conotoxin-ImI exhibits subtype-specific nicotinic acetylcholine receptor blockade: preferential inhibition of homomeric α7 and α9 receptors. Mol. Pharmacol. 48:194-199 (1995).
31. Blount, P., and J. Merlie. Molecular basis of the two nonequivalent ligand binding sites of the muscle nicotinic acetylcholine receptor. Neuron 3:349-357 (1989).
32. Corringer, P. J., J. L. Galzi, J. L. Eisele, S. Bertrand, J. P. Changeux, and D. Bertrand. Identification of a new component of the agonist binding site of the nicotinic alpha 7 homooligomeric receptor. J. Biol. Chem. 270: 11749-11752 (1995).
33. Helekar, S. A., D. Char, S. Neff, and J. Patrick. Prolyl isomerase requirement for the expression of functional homo-oligomeric ligand-gated ion channels. Neuron 12:179-189 (1994).
34. Vernallis, A. B., W. G. Conroy, and D. K. Berg. Neurons assemble acetylcholine receptors with as many as three kinds of subunits while maintaining subunit segregation among receptor subtypes. Neuron 10:451-464 (1993).
35. Ramirez-Latorre, J., C. R. Yu, X. Qu, F. Perin, A. Karlin, and L. Role. Functional contributions of α5 subunit to neuronal acetylcholine receptor channels. Nature (Lond.) 380:347-351. (1996).
36. Mandelzys, A., P. de Koninck, and E. Cooper. Agonist and toxin sensitivities of ACh-evoked currents on neurons expressing multiple nicotinic ACh receptor subunits. J. Neurophys. 74:1212-1221 (1995).
37. Forsayeth, J. R., E. Kobrin, B. D. Winegar, and D. J. Fitzgerald. The nicotinic acetylcholine receptor in cerebellum is composed of four different subunits. Soc. Neurosci. Abstr. 21:1583 (1995).
38. Colquhoun, L., K. Dineley, and J. Patrick. A hetero-beta neuronal nicotinic acetylcholine receptor expressed in Xenopus oocytes. Soc. Neurosci. Abstr. 19:1533 (1993).