A 53-base-pair inverted repeat negatively regulates expression of the adjacent and divergently oriented cytochrome P450(BM-1) gene and its regulatory gene, bm1P1, in Bacillus megaterium

Journal of Bacteriology

Volumn 179, Issue 1, 1997, Pages 280-283

  Shaw, Gwo Chyuan ^a   Sung, Chi Chang ^a   Liu, Chang Hsiesh ^a   Kao, Hsun Sheng ^a  

^a NATIONAL YANG MING UNIVERSITY   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME P450;

ARTICLE; BACILLUS MEGATERIUM; BASE PAIRING; GENE DELETION; GENE EXPRESSION REGULATION; GENE LOCATION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; REGULATOR GENE; STRUCTURAL GENE; TRANSCRIPTION REGULATION;

BACILLUS MEGATERIUM; BACILLUS SUBTILIS;

EID: 0031026583     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.1.280-283.1997     Document Type: Article

References (25)

1. MB-3 and other inducible bacterial P450 cytochromes: biochemistry and regulation. Annu. Rev. Pharmacol. Toxicol. 31:177-203.
2. Harlow, E., and D. Lane. 1988. Antibodies: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, N.Y.
3. He, J. S., and A. J. Fulco. 1991. A barbiturate-regulated protein binding to a common sequence in the cylochrome P450 genes of rodents and bacteria. J. Biol. Chem. 266:7864-7869.
4. BM-1 of Bacillus megaterium. J. Biol. Chem. 270:18615-18625.
5. BM-1 from Bacillus megatenum. Biochim. Biophys. Acta 1009:301-303.
6. Horwitz, M. S. Z., and L. A. Loeb. 1988. An E. coli promoter that regulates transcription by DNA superhelix-induced cruciform extrusion. Science 241:703-705.
7. Laemmli, U. K. 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
8. BM-3 and its two Functional domains. J. Biol. Chem. 266:11909-11914.
9. BM-3 in Bacillus megaterium by the binding of Bm3R1 repressor to barbie box elements and operator sites. J. Biol. Chem. 270:18606-18614.
10. BM-3 in Bacillus megaterium. J. Biol. Chem. 270:4438-4450.
11. 10a.Liu, C. H., and G. C. Shaw, Unpublished data.
12. Moran, C. P., Jr., N. Lang, S. F. J. LeGrice, G. Lee, M. Stephens, A. L. Sonenshein, J. Pero, and R. Losick. 1982. Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtills. Mol. Gen. Genet. 186:339-346.
13. Narhi, L. O., and A. J. Fulco. 1982. Phenobarbital induction of a soluble cytochrome P450-dependent fatty acid monooxygcnusc in Bacillus megaterium. J. Biol. Chem. 257:2147-2150.
14. Rauschenbach, R., M. Isernhasen, C. Noeske-Jungblut, W. Biodnl, and G. Siewert. 1993. Cloning sequencing and expression of the gene for cylochrome P450meg, the steroid-15β-monooxygenase from Bacillus megaterium ATCC 13368. Mol. Gen. Genet. 241:170-176.
15. BM-3, a prototype for microsomal P450's. Science 261:731-736.
16. Ray, C., R. E. Hay, H. L. Carter, and C. P. Moran, Jr. 1985. Mutations that affect utilization of a promoter in stationary-phase Bacillus subtilis. J. Bacteriol, 163:610-614.
17. BM-3, a single peptide cytochrome P450:NADPH-P450 reduclase from Bacillus megaterium. J. Biol. Chem. 264:10987-10995.
18. BM-1 from Bacillus megaterium ATCC 14581. Biochim. Biophys. Acta 838:302-311.
19. BM-3 gene of Bacillus megaterium by Bm3R1 protein. J. Biol. Chem, 267:5515-5526.
20. BM-3 gene of Bacillus megaterium. J. Biol. Chem. 268:2997-3004.
21. BM-3 in Bacillus megaterium. Curr. Microbiol. 32:124-128.
22. Shaw, W. V. 1975. Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria. Methods Enzymol. 43:737-755.
23. Towbin, H., T. Staehelin, and J. Gordon. 1979. Electrophorctic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350-4354.
24. Wen, L. P., and A. J. Fulco. 1987, Cloning of the gene encoding a catalytically self-sufficient cytochrome P450 fatty acid monooxygenase induced by barbi- turates in Bacillus megaterium and its functional expression and regulation in heterologous (Escherichia coli) and homologous (Bacillus megaterium) hosts. J. Biol. Chem. 262:6676-6682.
25. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103-109.