Generation of a membrane-bound, oligomerized pre-pore complex is necessary for pore formation by Clostridium septicum alpha toxin

Molecular Microbiology

Volumn 23, Issue 3, 1997, Pages 551-558

  Sellman, Bret R ^a   Kagan, Bruce L ^b   Tweten, Rodney K ^a  

^a UNIVERSITY OF OKLAHOMA COLLEGE OF MEDICINE   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA TOXIN;

ANIMAL CELL; ARTICLE; CELL MEMBRANE; CLOSTRIDIUM SEPTICUM; CONTROLLED STUDY; CYTOLYSIS; ERYTHROCYTE; NONHUMAN; PRIORITY JOURNAL; TEMPERATURE; TOXICITY TESTING;

EID: 0031025916     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1997.d01-1876.x     Document Type: Article

References (21)

1. Ballard, J., Bryant, A., Stevens, D., and Tweten, R.K. (1992) Purification and characterization of the lethal toxin (alpha toxin) of Clostridium septicum. Infect Immun 60: 784-790.
2. Ballard, J., Sokolov, Y., Yuan, W.-L., Kagan, B.L., and Tweten, R.K. (1993) Activation and mechanism of Clostridium septicum alpha toxin. Mol Microbiol 10: 627-634.
3. Ballard, J., Crabtree, J., Roe, B.A., and Tweten, R.K. (1995) The primary structure of Clostridium septicum alpha toxin exhibits similarity with Aeromonas hydrophila aerolysin. Infect Immun 63: 340-344.
4. Bhakdi, S., and Tranum-Jensen, J. (1991) Alpha toxin of Staphylococcus aureus. Microbiol Rev 55: 733-751.
5. Fussle, R., Bhakdi, S., Sziegoleit, A., Tranum-Jensen, J., Kranz, T., and Wellensiek, H. (1981) On the mechanism of membrane damage by staphylococcus aureus α-toxin. J Cell Biol 91: 83-94.
6. Garland, W., and Buckley, J. (1988) The cytolytic toxin aerolysin must aggregate to disrupt erythrocytes, and aggregation is stimulated by human glycophorin. Infect Immun 56: 1249-1253.
7. van der Goot, F., Pattus, F., Wong, K., and Buckley, J. (1993) Oligomerization of the channel-forming toxin aerolysin precedes insertion into lipid bilayers. Biochem 32: 2636-2642.
8. Harris, R.W., Sims, P.J., and Tweten, R.K. (1991) Kinetic aspects of the aggregation of Clostridium perfringens theta toxin on erythrocyte membranes: a fluorescence energy transfer study. J Biol Chem 266: 6936-6941.
9. Harshman, S., Boquet, P., Duflot, E., Alouf, J., Montecucco, C., and Papini, E. (1989) Staphylococcal α-toxin: a study of membrane penetration and pore formation. J Biol Chem 264: 14978-14984.
10. Keough, K.M.W., and Davis, P.J. (1984) Thermal analysis of membranes. Vol. 12. In Membrane Fluidity. Kates, M., and Manson, L.A. (eds). New York and London: Plenum Press, pp. 55-97.
11. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
12. Mirzabekov, T.A., Ballarin, C., Nicolini, M., Zatta, P., and Sorgato, M.C. (1993) Reconstitution of the native mitochondrial outer membrane in planar bilayers. Comparison with the outer membrane in a patch pipette and effect of aluminum compounds. J Membr Biol 133: 129-143.
13. Mirzabekov, T.A., Lin, M.C., and Kagan, B.L. (1996) Pore formation by the cytotoxic islet amyloid peptide amylin. J Biol Chem 271: 1988-1992.
14. Parker, M., Buckley, J.T., Postma, J., Tucker, A., Leonard, K., Pattus, F., and Tsernoglou, D. (1994) Structure of the Aeromonas toxin proaerolysin in its water-soluble and membrane-channel states. Nature 367: 292-295.
15. Parker, M.W., van der Goot, F.G., and Buckley, J.T. (1996) Aerolsyin, the ins and outs of a model channel-forming toxin. Mol Microbiol 19: 205-212.
16. Reichwein, J., Hugo, F., Roth, M., Sinner, A., and Bhakdi, S. (1987) Quantitative analysis of the binding and Oligomerization of staphylococcal alpha-toxin in target erythrocyte membranes. Infect Immun 55: 2940-2944.
17. Sambrook, J., Fritsch, E., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
18. Stevens, D.L., Musher, D.M., Watson, D.A., Eddy, H., Hamill, R.J., Gyorkey, F., Rosen, H., and Mader, J. (1990) Spontaneous, nontraumatic gangrene due to Clostridium septicum. Rev Infect Dis 12: 286-296.
19. Tweten, R.K. (1995) Pore-forming toxins of the gram positive bacteria. In Virulence Mechanisms of Bacterial Pathogens. Roth, J.A., Bolin, C.A., Brogden, K.A., Minion, C., and Wannemuehler, M.J. (eds). Washington DC: American Society for Microbiology, pp. 207-230.
20. Valeva, A., Palmer, M., Hilgert, K., Kehoe, M., and Bhakdi, S. (1995) Correct oligomerization is a prerequisite for insertion of the central molecular domain of staphylococcal α-toxin into the lipid bilayer. Biochim Biophys Acta 1236: 213-218.
21. Walker, B., Krishnasastry, M., Zorn, L., and Bayley, H. (1992) Assembly of the oligomeric membrane pore formed by staphylococcal alpha-hemolysin examined by truncation mutagenesis. J Biol Chem 267: 21782-21786.