Cloning, sequencing and functional expression of dihydrolipoamide dehydrogenase from the human pathogen Trypanosoma cruzi

European Journal of Biochemistry

Volumn 243, Issue 3, 1997, Pages 739-747

  Schoneck, R ^a   Billaut Mulot, O ^a   Numrich, P ^a   Ouaissi, M A ^a   Krauth Siegel, R L ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

Flavoprotein; Lipoamide dehydrogenase; Mitochondrial targeting; Oxidoreductase; Trypanosoma

Indexed keywords

ANTIPARASITIC AGENT; COMPLEMENTARY DNA; DIHYDROLIPOAMIDE DEHYDROGENASE; NIFURTIMOX;

ALLELE; AMINO ACID SEQUENCE; ARTICLE; CELL CULTURE; CITRIC ACID CYCLE; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME SUBUNIT; ESCHERICHIA COLI; GENE EXPRESSION; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; LIFE CYCLE; MOLECULAR CLONING; PRIORITY JOURNAL; TRYPANOSOMA CRUZI;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MAMMALIA; TRYPANOSOMA; TRYPANOSOMA BRUCEI; TRYPANOSOMA CRUZI; TRYPANOSOMATIDAE;

EID: 0031024753     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.00739.x     Document Type: Article

References (52)

1. Allison, N., Williams, C. H. Jr & Guest, J. R. (1988) Overexpression and mutagenesis of the lipoamide dehydrogenase of Escherichia coli, Biochem J. 256, 741–749.
2. Borst, P. (1986) Discontinuous transcription and antigenic variation in trypanosomes, Annu. Rev. Biochem. 55, 701–732.
3. Boveris, R., Sies, H., Martino, E. E., Docampo, R., Turrens, J. F. & Stoppani, A. O. M. (1980) Deficient metabolic utilization of hydrogen peroxide in Trypanosoma cruzi, Biochem. J. 188, 643–648.
4. Bunik, V., Shoubnikova, A., Loeffelhardt, S., Bisswanger, H., Borbe, H. O. & Follmann, H. (1995) Using lipoate enantiomers and thioredoxin to study the mechanism of the 2‐oxoacid‐dependent dihydrolipoate production by the 2‐oxoacid dehydrogenase complexes, FEBS Lett. 371, 167–170.
5. Carnieri, E. G. S., Moreno, S. N. J. & Docampo, R. (1993) Trypanothione‐dependent peroxide metabolism in Trypanosoma cruzi different stages, Mol. Biochem. Parasitol. 61, 79–86.
6. Clayton, C. & Hotz, H.‐R. (1996) Post‐transcriptional control of PARP gene expression, Mol. Biochem. Parasitol. 77, 1–6.
7. Cornette, J., Capron, A. & Ouaissi, M. A. (1988) Trypanosoma cruzi: fibronectin promotes uptake of epimastigote culture forms by human neutrophils and monocytes. Int. Arch. Allergy appl. Immunol. 86, 139–146.
8. Danson, M. J., Conroy, K., McQuattie, A. & Stevenson, K. J. (1987) Dihydrolipoamide dehydrogenase from Trypanosoma brucei, Biochem. J. 243, 661–665.
9. Diaz, F. & Komuniecki, R. (1995) Pyruvate dehydrogenase complex from the primitive insect trypanosomatid. Crithidia fasciculata: dihydrolipoyl dehydrogenase‐binding protein has multiple lipoyl domains, Mol. Biochem. Parasitol. 75, 87–97.
10. Dietrichs, D. & Andreesen, J. R. (1990) Purification and comparative studies on dihydrolipoamide dehydrogenase from the anaerobic, glycine utilizing bacteria Peptostreptococcus glycinophilus, Clostridium cylindrosporum, and Clostridium sporogenes, J. Bacteriol. 172, 243–251.
11. Docampo, R. & Stoppani, A. O. M. (1979) Generation of superoxide anion and hydrogen peroxide induced by nifurtimox in Trypanosoma cruzi, Arch. Biochem. Biophys. 197, 317–321.
12. Else, A. J., Hough, D. W. & Danson, M. J. (1993) Cloning, sequencing, and expression of Trypanosoma brucei dihydrolipoamide dehydrogenase, Eur. J. Biochem. 212, 423–429.
13. Fairlamb, A. H. & Cerami, A. (1992) Metabolism and functions of trypanothione in the Kinetoplastida, Annu. Rev. Microbiol. 46, 695–729.
14. Fairlamb, A. H., Smith, K. & Hunter, K. J. (1992) The interaction of arsenical drugs with dihydrolipoamide and dihydrolipoamide dehydrogenase from arsenical resistant and sensitive strains of Trypanosoma brucei brucei, Mol. Biochem. Parasitol. 53, 223–232.
15. Frey, P. Y., Ikeda, B. H., Gavino, G. R., Speckhard, D. C. & Wong, S. S. (1978) Escherichia coli pyruvate dehydrogenase complex, J. Biol. Chem. 253, 7234–7241.
16. Gasdaska, P. Y., Gasdaska, J. R., Cochran, S. & Powis, G. (1995) Cloning and sequencing of a human thioredoxin reductase, FEBS Lett. 373, 5–9.
17. Guest, J. R., Lewis, H. M., Graham, L. D., Packman, L. C. & Perham, R. N. (1985) Genetic reconstruction and functional analysis of the repeating lipoyl domains in the pyruvate dehydrogenase multienzyme complex of Escherichia coli, J. Mol. Biol. 185, 743–754.
18. Hauser, R., Pypaert, M., Hausler, T., Horn, E. K. & Schneider, A. (1996) In vitro import of proteins into mitochondria of Trypanosoma brucei and Leishmania turentolae, J. Cell Sci. 109, 517–523.
19. Hendrick, J. P., Hodges, P. E. & Rosenberg, L. E. (1989) Survey of amino‐terminal proteolytic cleavage sites in mitochondrial precursor proteins: Leader peptides cleaved by two matrix proteases share a three‐amino acid motif, Proc. Natl Acad. Sci. USA 86, 4056–4060.
20. Hopkins, N. & Williams, C. H. Jr (1995) Characterization of lipoamide dehydrogenase from Escherichia coli lacking the redox active disulfide: C44S and C49S, Biochemistry 34, 11751–11765.
21. Huaug, J. & Van der Ploeg, L. H. T. (1991) Requirement of a polypyrimidine tract for trans ‐splicing in trypanosomes: discriminating the PARP promotor from the immediately adjacent 3′ splice acceptor site, EMBO J. 10, 3877–3885.
22. Jackman, S. A., Hough, D. W., Danson, M. J., Stevenson, K. J. & Opperdoes, F. R. (1990) Subcellular localisation of dihydrolipoamide dehydrogenase and detection of lipoic acid in bloodstream forms of Trypanosoma brucei, Eur. J. Biochem. 193, 91–95.
23. Kagan, V. E., Shvedova, A., Serbinova, E., Khan, S., Swanson, C., Powell, R. & Packer, L. (1992) Dihydrolipoic acid–a universal antioxidant both in the membrane and in the aqueous phase, Biochem. Pharmacol. 44, 1637–1649.
24. Kim, H., Liu, T.‐C. & Patel, M. S. (1991) Expression of cDNA sequences encoding mature and precursor forms of human dihydrolipoamide dehydrogenase in Escherichia coli, J. Biol. Chem. 266, 9361–9373.
25. Kim, H. & Patel, M. S. (1992) Characterization of two site‐specifically mutated human dihydrolipoamide dehydrogenases (His‐452→Gln and Glu‐457→Gln), J. Biol. Chem. 267, 5128–5132.
26. Korotchkina, L. G., Tucker, M. M., Thekkumkara, T. J., Madhusudhan, K. T., Pons, G., Kim, H. & Patel, M. S. (1995) Overexpression and characterization of human tetrameric pyruvate dehydrogenase and its individual subunits, Protein Expr. Purif. 6, 79–90.
27. Krauth‐Siegel, R. L. & Schijneck, R. (1995) Trypanothione reductase and lipoamide dehydrogenase as targets for a structure‐based drug design, FASEB J. 9, 1138–1146.
28. Lohrer, H. & Krauth‐Siegel, R. L. (1990) Purification and characterization of lipoamide dehydrogenase from Trypanosoma cruzi, Eur. J. Biochem. 194, 863–869.
29. Lorimer, G. H. (1996) A quantitative assessment of the role of the chaperonin proteins in protein folding in vivo, FASEB J. 10, 5–9.
30. Maeda‐Yorita, K., Russel, G. C., Guest, J. R., Massey, V. & Williams, C. H. Jr (1994) Modulation of the oxidation‐reduction potential of the flavin in lipoamide dehydrogenase from Escherichia coli by alteration of a nearby charged residue, K53R, Biochemistry 33, 6213–6220.
31. Maeng, C.‐Y., Yazdi, M. A., Niu, X.‐D., Lee, H. Y. & Reed, L. J. (1994) Expression, purification, and characterization of the dihydrolipoamide dehydrogenase‐binding protein of the pyruvate dehydrogenase complex from Saccharomyces cerevisiae, Biochemistry 33, 13801–13807.
32. Maizels, R. M., Blaxter, M. L., Robertson, B. D. & Selkirk, M. E. (1991) Parasite antigens, parasite genes, Cambridge University Press, Cambridge, UK.
33. Matsugo, S., Yan, L.‐J., Han, D., Trischler, H. J. & Packer, L. (1995) Elucidation of antioxidant activity of α‐lipoic acid toward hydroxyl radical, Biochem. Biophys. Res. Commun. 208, 161–167.
34. Mattevi, A., Schierbeek, A. J. & Hol, W. G. J. (1991) Refined crystal structure of lipoamide dehydrogenase from Azotobacrer vinelandii at 2.2 Å resolution, J. Mol. Biol. 220, 975–994.
35. Mattevi, A., Obmolova, G., Sokatch, J. R., Betzel, C & Hol, W. G. J. (1992) The refined crystal structure of Pseudoomonas putida lipoamide dehydrogenase complexed with NAD + at 2.45Å resolution. Proteins Struct. Funct. Genet. 13, 336–351.
36. Mattevi, A., Obmolova, G., Kalk, K. H., van Berkel, W. J. H. & Hol, W. G. J. (1993), Three‐dimensional structure of lipoamide dehydrogenase from Pseudomonas fluorescens at 2.8Å resolution, J. Mol. Biol. 230, 1200–1215.
37. Otulakowski, G. & Robinson, B. H. (1987) Isolation and sequence determination of cDNA clones for porcine and human lipoamide dehydrogenase, J. Biol. Chem. 262, 17313–17318.
38. Ouaissi, M. A., Cornette, J. & Capron, A. (1986) Identification and isolation of Trypanosoma cruzi trypomastigote cell surface protein with properties expected of a fibronectin receptor, Mol. Biochem. Parasitol. 19, 201–211.
39. Priest, J. W. & Hajduk, S. L. (1995) The trypanosomatid Rieske iron‐sulfur proteins have a cleaved presequence that may direct mitochondrial import, Biochim. Biophys. Acta 1269, 201–204.
40. Reed, L. J., Koike, M., Levitch, M. E. & Leach, E R. (1958) Studies on the nature and reactions of protein‐bound lipoic acid, J. Biol. Chem. 232, 143–158.
41. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989) Molecular cloning: a laboratory manual, 2nd edn, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
42. Sanger, F., Nicklen, S. & Coulson, A. R. (1977) DNA sequencing with chain‐terminating inhibitors, Proc. Natl Acad. Sci. USA 74, 5463–5467.
43. Santiago, A. R., Afchain, D. & Capron, A. (1981) Specific antigens of Trypanosoma cruzi amastigotes and trypomastigotes, Ann. Soc. Belge Med. Trop. 61, 369–378.
44. Schirmer, R. H., Muller, J. G. & Krauth‐Siegel, R. L. (1995) Disulfide‐reductase inhibitors as chemotherapeutic agents: The design of drugs for trypanosomiasis and malaria, Angew. Chenm. Int. Ed. Engl. 34, 141–154.
45. Scholich, H., Murphy, M. E. & Sies, H. (1989) Antioxidant activity of dihydrolipoate against microsomal lipid peroxidation and its dependence on α‐tocopherol, Biochim. Biophys. Acta 1001, 256–261.
46. Sreider, C. M., Grinblat, L. & Stoppani, A. O. M. (1992) Reduction of nitrofuran compounds by heart lipoamide dehydrogenase: role of flavin and the reactive disulfide groups, Biochem. Int. 28, 323–334.
47. Torri, A. F. & Englund, P. T. (1995) A DNA polymerase β in the mitochondrion of the trypanosomatid Crithidia fasciculuta, J. Biol. Chem. 270, 3495–3497.
48. Tsai, C. S. & Wand, A. J. (1992) pH dependent kinetic studies of lipoamide dehydrogenase catalysis, Int. J. Biochem. 24, 1801–1806.
49. Westphal, A. H. & de Kok, A. (1988) Lipoamide dehydrogenase from Azotobacter vinelandii, Em J. Biochem. 172, 299–305.
50. Williams, C. H. Jr (1992) Lipoamide dehydrogenase, glutathione reductase, thioredoxin reductase, and mercuric ion reductase–a family of flavoenzyme transhydrogenases, in Chemistry and biochemistry of flavoenzymes ( Müller, F., ed.) vol. 3, pp. 121–211, CRC Press, Boca Raton.
51. Wynn, R. M., Davie, J. R., Cox, R. P. & Chuang, D. T. (1992) Chaperonins GroEL and GroES promote assembly of heterotetramers (α 2 β 2) of mammalian mitochondrial branched‐chain α‐keto acid decarboxylase in Escherichia coli, J. Biol. Chem. 267, 12400–12403.
52. Xu, C. & Ray, D. S. (1993) Isolation of proteins associated with kineto‐plast DNA networks in vivo, Proc. Natl Acad. Sci. USA 90, 1786–1789.