Cellular and regional distribution of the glutamate transporter GLAST in the CNS of rats: Nonradioactive in situ hybridization and comparative immunocytochemistry

Journal of Neuroscience

Volumn 17, Issue 1, 1997, Pages 1-10

  Schmitt, Angelika ^a   Asan, Esther ^a   Püschel, Bernd ^a   Kugler, Peter ^a,b  

^a UNIVERSITY OF WÜRZBURG   (Germany)

^b Institute of Anatomy   (Germany)

Author keywords

astrocytes; ependymal cells; glutamate transporter GLAST

Indexed keywords

ALKALINE PHOSPHATASE; CARRIER PROTEIN; DIGOXIGENIN; GLUTAMIC ACID; MESSENGER RNA; POLYCLONAL ANTIBODY;

ANIMAL TISSUE; ARTICLE; ASTROCYTE; CELLULAR DISTRIBUTION; CENTRAL NERVOUS SYSTEM; CONTROLLED STUDY; EPENDYMA CELL; GLIA CELL; IMMUNOCYTOCHEMISTRY; IN SITU HYBRIDIZATION; MALE; NERVE CELL; NONHUMAN; OLIGONUCLEOTIDE PROBE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; RAT; RNA PROBE;

EID: 0031024715     PISSN: 02706474     EISSN: None     Source Type: Journal    
DOI: 10.1523/jneurosci.17-01-00001.1997     Document Type: Article

References (24)

1. Asan E, Kugler P (1995) Qualitative and quantitative detection of alkaline phosphatase coupled to an oligonucleotide probe for somatostatin mRNA after in situ hybridization using unfixed rat brain tissue. Histochem Cell Biol 103:463-471.
2. Atherton E, Logan CJ, Sheppard RC (1981) Peptide synthesis, Part 2. Procedures for solid-phase synthesis using Nafluorenylmethoxycarbonylamino-acids on polyamide supports: synthesis of substance P and of acyl carrier protein 65-74 decapeptide. J Chem Soc Perkin Trans I 538-546.
3. Barbour B, Keller BU, Llano I, Marty A (1994) Prolonged presence of glutamate during excitatory synaptic transmission to cerebellar Purkinje cells. Neuron 12:1331-1343.
4. Bignami A, Eng LF, Dahl D, Uyeda CT (1972) Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence. Brain Res 43:429-435.
5. Burnette WN (1981) Western blotting: electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radionated protein A. Anal Biochem 112:195-203.
6. Chaudhry FA, Lehre KP, van Lookeren Campagne M, Ottersen OP, Danbolt NC, Storm-Mathisen J (1995) Glutamate transporters in glial plasma membranes: highly differentiated localizations revealed by quantitative ultrastructural immunocytochemistry. Neuron 15:711-720.
7. Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156-159.
8. Conradt M, Storck T, Stoffel W (1995) Localization of N-glycosylation sites and functional role of the carbohydrate units of GLAST-1, a cloned rat brain L-glutamate/L-aspartate transporter. Eur J Biochem 229:682-687.
9. Dågerlind A, Friberg K, Bean AJ, Hökfelt T (1992) Sensitive mRNA detection using unfixed tissue: combined radioactive and non-radioactive in situ hybridization histochemistry. Histochemistry 98:39-49.
10. Derouiche A, Rauen T (1995) Coincidence of L-glutamate/L-aspartate transporter (GLAST) and glutamine synthetase (GS) immunoreactions in retinal glia: evidence for coupling of GLAST and GS in transmitter clearance. J Neurosci Res 42:131-143.
11. Drenckhahn D, Franz H (1986) Identification of actin-, α-actinin-, and vinculin-containing plaques at the lateral membrane of epithelial cells. J Cell Biol 102:1843-1852.
12. Drenckhahn D, Jöns T, Schmitz F (1993) Production of polyclonal antibodies against proteins and peptides. Methods Cell Biol 37:7-56.
13. Fagg GE, Foster AC (1983) Amino acid neurotransmitters and their pathways in the mammalian central nervous system. Neuroscience 9:701-719.
14. Fonnum F (1984) Glutamate: A neurotransmitter in mammalian brain. J Neurochem 42:1-11.
15. Kanai Y, Hediger MA (1992) Primary structure and functional characterization of a high-affinity glutamate transporter. Nature 360:467-471.
16. Kanai Y, Smith CP, Hediger MA (1993) The elusive transporters with a high affinity for glutamate. Trends Neurosci 16:365-370.
17. Kanner BI, Schuldiner S (1987) Mechanism of transport and storage of neurotransmitters. CRC Crit Rev Biochem 22:1-38.
18. Kugler P (1993) Enzymes involved in glutamatergic and GABAergic neurotransmission. Int Rev Cytol 147:285-336.
19. Kugler P, Weeger T, Horváth E (1995) Glutamate dehydrogenase in astrocytes of the rat dentate gyrus following lesion of the entorhinal cortex. Neuroscience 64:173-182.
20. Lehre KP, Levy LM, Ottersen OP, Storm-Mathisen J, Danbolt NC (1995) Differential expression of two glial glutamate transporters in the rat brain: quantitative and immunocytochemical observations. J Neurosci 15:1835-1853.
21. Maxwell MH (1978) Two rapid and simple methods used for the removal of resins from 1.0 μm thick epoxy sections. J Microsc 112:253-255.
22. Paxinos G, Watson C (1986) The rat brain in stereotaxic coordinates, 2nd Edition. New York: Academic.
23. Vidal S, Lombardero M, Sánchez P, Román A, Moya L (1995) An easy method for the removal of Epon resin from semithin sections: application of the avidin-biotin technique. Histochem J 27:204-209.
24. Würdig S, Kugler P (1991) Histochemistry of glutamate metabolizing enzymes in the rat cerebellar cortex. Neurosci Lett 130:65-168.