Heterogeneity of nicotinic receptor class and subunit mRNA expression among individual parasympathetic neurons from rat intracardiac ganglia

Journal of Neuroscience

Volumn 17, Issue 2, 1997, Pages 586-596

  Poth, Kevin ^a   Nutter, Thomas J ^a   Cuevas, Javier ^a,b   Parker, Michael J ^a   Adams, David J ^a,c   Luetje, Charles W ^a  

^a UNIVERSITY OF MIAMI MILLER SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

cardiac ganglia; neuronal nicotinic receptors; nicotinic acetylcholine receptors; nicotinic pharmacology; parasympathetic ganglia; single cell RT PCR

Indexed keywords

NICOTINIC RECEPTOR; RECEPTOR SUBUNIT;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; NONHUMAN; PARASYMPATHETIC GANGLION; PARASYMPATHETIC NERVE CELL; PRIORITY JOURNAL; RAT; RNA ANALYSIS; RNA SYNTHESIS;

EID: 0031021358     PISSN: 02706474     EISSN: None     Source Type: Journal    
DOI: 10.1523/jneurosci.17-02-00586.1997     Document Type: Article

References (28)

1. Adams DJ, Nutter TJ (1992) Calcium permeability and modulation of nicotinic acetylcholine receptor channels in rat parasympathetic neurons. J Physiol (Paris) 86:67-76.
2. Allen TGJ, Hassall CJS, Burnstock G (1994) Mammalian intrinsic cardiac neurons in cell culture. In: Neurocardiology (Armour JA, Ardell JL, eds), pp 115-138. New York: Oxford UP.
3. Anand R, Conroy WG, Schoepfer R, Whiting P, Lindstrom J (1991) Neuronal nicotinic acetylcholine receptors expressed in Xenopus oocytes have a pcntameric quaternary structure. J Biol Chem 266:11192-11198.
4. Ardell JL (1994) Structure and function of mammalian intrinsic cardiac neurons. In: Neurocardiology (Armour JA, Ardell JL, eds), pp 95-114. New York: Oxford UP.
5. Ardell JL, Randall WC (1986) Selective vagal innervation of sinoatrial and atrioventricular nodes in canine hearts. Am J Physiol 251:H764-H773.
6. Armour JA (1991) Intrinsic cardiac neurons. J Cardiovasc Electrophysiol 2:331-341.
7. Boulter J, Connolly J, Deneris E, Goldman D, Heinemann S, Patrick J (1987) Functional expression of two neuronal nicotinic acetylcholine receptors from cDNA clones identifies a gene family. Proc Natl Acad Sci USA 84:7763-7767.
8. Boyd RT, Jacob MH, Couturier S, Ballivet M, Berg DK (1988) Expres-sion and regulation of neuronal acetylcholine receptor mRNA in chick ciliary ganglia. Neuron 1:495-502.
9. Butler CK, Smith FM, Cardinal R, Murphy DA, Hopkins DA, Armour JA (1990) Cardiac responses to electrical stimulation of discrete loci in canine atrial and ventricular ganglionated plexi. Am J Physiol (Heart Circ Physiol 28) 259:H1108-H1117.
10. Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid-guanidinium-thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156-159.
11. Colquhoun L, Dineley K, Patrick J (1993) A hetero-beta neuronal nicotinic acetylcholine receptor expressed in Xenopus oocytes. Soc Neurosci Abstr 19:1533.
12. Connolly JG, Gibb AJ, Colquhoun D (1995) Heterogeneity of neuronal nicotinic acetylcholine receptors in thin slices of rat medial habenula. J Physiol (Lond) 484:87-105.
13. Cooper E, Couturier S, Ballivet M (1991) Pentameric structure and subunit stoichiometry of a neuronal nicotinic acetylcholine receptor. Nature 350:235-238.
14. Covernton PJO, Kojima H, Sivilotti LG, Gibb AJ, Colquhoun D (1994) Comparison of neuronal nicotinic receptors in rat sympathetic neurones with subunit pairs expressed in Xenopus oocytes. J Physiol (Lond) 481:27-34.
15. Duvoisin RM, Deneris ES, Boulter J, Patrick J, Heinemann S (1989) The functional diversity of the neuronal nicotinic acetylcholine receptors is increased by a novel subunit: β4. Neuron 3:487-496.
16. Ehinger B, Falck B, Persson H, Sporrono B (1968) Adrenergic and cholinesterase-containing neurons of the heart. Histochimie 16:197-205.
17. Elgoyhen AB, Johnson DS, Boulter J, Vetter DE, Heinemann S (1994) α9: an acetylcholine receptor with novel pharmacological properties expressed in rat cochlear hair cells. Cell 79:705-715.
18. Fieber LA, Adams DJ (1991) Acetylcholine-evoked currents in cultured neurones dissociated from rat parasympathetic cardiac ganglia. J Physiol (Lond) 434:215-237.
19. Flores CM, Rogers SW, Pabreza LA, Wolfe BB, Kellar KJ (1991) A subtype of nicotinic cholinergic receptor in rat brain is composed of α4 and β2 subunits and is up-regulated by chronic nicotine treatment. Mol Pharmacol 41:31-37.
20. Forsayeth JR, Kobrin E, Winegar BD, Fitzgerald DJ (1995) The nicotinic acetylcholine receptor in cerebellum is composed of four different subunits. Soc Neurosci Abstr 21:1583.
21. Hamill OP, Marty A, Neher E, Sakmann B, Sigworth F (1981) Improved patch-clamp techniques for high resolution current recordings from cells and cell-free membrane patches. Pflügers Arch 391:85-100.
22. Jacobowitz D (1967) Histochemical studies of the relationship of chromaffin cell and adrenergic nerve fibers to the cardiac ganglia of several species. J Pharmacol Exp Ther 158:227-240.
23. Steele PA, Gibbins IL, Morris JL (1996) Projections of intrinsic cardiac neurons to different targets in the guinea-pig heart. J Auton Nerv Syst 56:191-200.
24. Ullian EM, Sargent PB (1995) Pronounced cellular diversity and extra-synaptic location of nicotinic acetylcholine receptor subunit immunore-activities in the chicken pretectum. J Neurosci 15:7012-7023.
25. Vernallis AB, Conroy WG, Berg DK (1993) Neurons assemble acetylcholine receptors with as many as three kinds of subunits while maintaining subunit segregation among receptor subtypes. Neuron 10:451-464.
26. Wada K, Ballivet M, Boulter J, Connolly J, Wada E, Deneris ES, Swanson LW, Heinemann S, Patrick J (1988) Functional expression of a new pharmacological subtype of brain nicotinic acetylcholine receptor. Science 240:330-334.
27. Wilson Horch HL, Sargent PB (1996) Perisynaptic surface distribution of multiple classes of nicotinic acetylcholine receptors on neurons in the chicken ciliary ganglion. J Neurosci 15:7778-7795.
28. Yuan B-Y, Ardell JL, Hopkins DA, Losier AM, Armour JA (1994) Gross and microscopic anatomy of the canine intrinsic cardiac nervous system. Anat Rec 239:75-87.