Immunocytochemical localization of phenolic compounds in pollen walls using antibodies against p-coumaric acid coupled to bovine serum albumin

Protoplasma

Volumn 197, Issue 3-4, 1997, Pages 148-159

  Niester Nyveld, C ^a   Haubrich, A ^a   Kampendonk, H ^a   Gubatz, S ^a   Tenberge, K B ^a   Rittscher, M ^a   Wilmesmeier, S ^a   Wiermann, R ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

Antibodies; Exine; Immunocytochemistry; Phenols; Pollen; Sporopollenin

Indexed keywords

AVES; BOVINAE; CUCURBITA MAXIMA; MELEAGRIS GALLOPAVO; ORYCTOLAGUS CUNICULUS;

EID: 0031020987     PISSN: 0033183X     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF01288024     Document Type: Article

References (42)

1. 20. Planta 159: 1-6
2. Bäumker PA, Arendt S, Wiermann R (1988a) Metabolism of ferulic acid sucrose esters in anthers of Tulipa ev. Apeldoorn. I. The accumulation of esters and free sugars. Z Naturforsch 43c: 641-646
3. _ (1988b) Metabolism of ferulic acid sucrose esters in anthers of Tulipa cv. Apeldoorn. II. Highly specific degradation of the esters by different esterases activities. Z Naturforsch 43c: 647-655
4. Bokern M, Witte L, Wray V, Nimtz M, Meurer-Grimes B (1995) Trisubstituted hydroxycinnamic acid spermidines from Quercus dentata pollen. Phytochemistry 39: 1371-1375
5. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254
6. Erlanger BF (1980) The preparation of antigenic hapten-carrier-conjugates: a survey. In: Van Vunakis H, Langone JJ (eds) Methods in enzymology, vol 70, immunological techniques, part A. Academic Press, New York, pp 85-104
7. 13C NMR spectroscopy. Phytochemistry 28: 751-753
8. Fujiwara K, Matsumoto N, Kitagawa T, Inouye K (1990) The use of N-(aminobenzoylosy) succimide as a two-level heterobifunctional agent for the preparation of hapten-protein-conjugates. Daunomycin as a model hapten with an amino group. J Immunol Methods 134: 227-235
9. Greve B, Yang Y, Steup M (1991) Monoclonal antibodies directed against a heteroglycan from higher plants. Plant Physiol 10: 187-194
10. 14C-phenylalanine in comparison to other precursors. Bot Acta 105: 407-413
11. 14C]-phenylalanine. Z Naturforsch 48c: 10-15
12. _ Herminghaus S, Meurer B, Strack D, Wiermann R (1986) The location of hydroxycinnamic acid amides in the exine of Corylus pollen. Pollen Spores 28: 347-354
13. _ Rittscher M, Meuter A, Nagler A, Wiermann R (1993) Tracer experiments of sporopollenin biosynthesis. Grana Suppl 1: 12-17
14. 13C MNR spectroscopy of sporopollenins from different plant taxa. Plant Physiol 86: 134-136
15. Harlow E, Lane D (eds) (1988) Antibodies: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor
16. Haubrich A, Kampendonk H, Rittscher M, Tenherge KB, Wiermann R (1994) Localization of phenolic compounds in pollen walls of different species by antibodies against p-coumaric acid-BSA-conjugates. In: Heberle-Bors E, Hesse M, Vicente O (eds) Frontiers in sexual plant reproduction research. 13th International Congress on Sexual Plant Reproduction, July 10-14 1994, Vienna, Austria, p 79
17. Hemsley AR, Chaloner WG, Scott AC, Groombridge CJ (1992) Carbon-13 solid-state nuclear magnetic resonance of sporopollenins from modern and fossil plants. Ann Bot 69: 545-549
18. _ Barrie PJ, Chaloner WG, Scott AC (1993) The composition of sporopollenin and its use in living and fossil plant systematics. Grana Suppl 1: 2-11
19. Herminghaus S, Gubatz S, Arendt S, Wiermann R (1988) The occurrence of phenols as degradation products of natural sporopollenin: a comparison with "synthetic sporopollenin". Z Naturforsch 43c: 491-500
20. Lamoureux SW, Vacha WEK, Ibrahim RK (1986) Localization of partially methylated flavonol glucosides in Chrysosplenium americanum. I. Preparation and some properties of a trimethyl flavonol glucoside antibody. Plant Sci 44: 169-173
21. Langone JJ, Gijka HB, van Vunakis H (1973) Nicotine and its metabolites: radioimmunoassays for nicotine and continine. Biochemistry 12: 5025-5030
22. Meurer B, Wiermann R, Strack D (1988a) Phenylpropanoid patterns in fagales pollen and their phylogenetic relevance. Phytochemistry 27: 823-828
23. _ (1988b) Hydroxycinnamic acid-spermidine amides from pollen of Alnus, glutinosa, Betula verrucosa and Pterocarya fraxinifolia. Phytochemistry 27: 839-843
24. Niester-Nyveld C, Steup M, Wiermann R (1993) Immunochemical characterization of p-coumaric acid conjugates in higher plants. Eur J Cell Biol 60 Suppl 37: 111
25. Nimtz M, Bokern M, Meurer-Grimes B (1996) Minor hydroxycinnamic acid spermidines from pollen of Quercus dentata. Phytochemistry 43: 487-489
26. Rasmussen SE (1990) Covalent immobilization of biomolecules onto polystyrene Micro Wells for use in biospecific assay. Ann Biol Clin 48: 647-650
27. Read SM, Northcote DH (1981) Minimization of variation in the response to different proteins of the Coomassie Blue G dye-binding assay for protein. Anal Biochem 116: 53-64
28. Rittscher M, Wiermann R (1988) Studies on sporopollenin biosynthesis in Tulipa anthers. II. Incorporation of precursors and degradation of the radiolabeled polymer. Sex Plant Reprod 1: 132-139
29. Schulze Osthoff K, Wiermann R (1987) Phenols as integrated compounds of sporopollenin from Pinus pollen. J Plant Physiol 131: 5-15
30. Skene DS, Browning G, Jones HJ (1987) Model-systems for the immunolocalisation of cis, trans abscisic acid in plant tissues. Planta 172: 192-199
31. Southworth D, Singh MB, Hough T, Smart IJ, Taylor P, Knox RB (1988) Antibodies to pollen exines. Planta 176: 482-487
32. Tenberge KB, Eising R (1995) Immunogold labelling indicates high catalase concentrations in amorphous and crystalline inclusions of sunflower (Helianthus annuus L.) peroxisomes. Histochem J 27: 184-195
33. Van der Meer JM, Stam ME, van Tunen AJ, Mol JNM, Stuitje AR (1992) Antisense inhibition of flavonoid biosynthesis in Petunia anthers results in male sterility. Plant Cell 4: 253-262
34. Vogt T, Wollenweber E, Taylor LP (1995) The structural requirements of flavonols that induce pollen germination of conditionally male fertile Petunia. Phytochemistry 38: 589-592
35. Wehling K, Niester C, Boon JJ, Willemse MTM, Wiermann R (1989) p-Coumaric acid - a monomer in the sporopollenin skeleton. Planta 179: 376-380
36. Weiler EW (1980) Radioimmunoassays for the differential and direct analysis of free and conjugated abscisic acid in plant extracts. Planta 148: 262-272
37. Wiermann R, Gubatz S (1992) Pollen wall and sporopollenin. Int Rev Cytol 140: 35-72
38. _ Vieth K (1983) Outer pollen wall, an important accumulation site of flavonoids. Protoplasma 118: 230-233
39. 13C CP/MAS NMR spectroscopic investigations on sporopollenin of different systematic origins. Z Naturforsch 48c: 697-701
40. Ylstra B, Touraev A, Moreno RMB, Stöger E, van Tunen AJ, Vincente O, Mol JNM, Heberle-Bors E (1992) Flavonols stimulate development, germination, and tube growth of tobacco pollen. Plant Physiol 100: 902-907
41. _ Busscher J, Franken J, Hollman PCH, Mol JNM, van Tunen AJ (1994) Flavonols and fertilization in Petunia hybrida: localization and mode of action during pollen tube growth. Plant J 6: 1-12
42. Zerback R, Dressler K, Hess D (1989) Flavonoid compounds from pollen and stigma of Petunia hybrida: inducers of the vir region of the Agrobacterium tumefaciens Ti plasmid. Plant Sci 62: 83-91