Evaluation of synovial cytokine patterns in rheumatoid arthritis and osteoarthritis by quantitative reverse transcription polymerase chain reaction

Rheumatology International

Volumn 16, Issue 5, 1997, Pages 191-196

  Wagner, S ^a   Fritz, P ^b   Einsele, H ^a   Sell, S ^a   Saal, J G ^a,c  

^a UNIVERSITY OF TÜBINGEN   (Germany)

^b Department of Pathology   (Germany)

^c Malteser St Franziskus Hospital gGmbH   (Germany)

Author keywords

Competitive RT PCR; Cytokine; Rheumatoid arthritis

Indexed keywords

CYTOKINE; INTERLEUKIN 10; INTERLEUKIN 1BETA; INTERLEUKIN 2; INTERLEUKIN 4; INTERLEUKIN 5; INTERLEUKIN 6; RNA; TUMOR NECROSIS FACTOR ALPHA;

ARTICLE; B LYMPHOCYTE; CELL INFILTRATION; CLINICAL ARTICLE; CONTROLLED STUDY; GENE EXPRESSION; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; MACROPHAGE; OSTEOARTHRITIS; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RHEUMATOID ARTHRITIS; SYNOVIUM; T LYMPHOCYTE;

ARTHRITIS, RHEUMATOID; B-LYMPHOCYTES; CYTOKINES; DNA PRIMERS; GENE EXPRESSION; HUMANS; IMMUNOHISTOCHEMISTRY; OSTEOARTHRITIS; POLYMERASE CHAIN REACTION; SYNOVIAL MEMBRANE; T-LYMPHOCYTES;

EID: 0031020841     PISSN: 01728172     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF01330295     Document Type: Article

References (27)

1. Amano Y, Lee SW, Allison AC (1993) Inhibition by glucocoticoids of the formation of interleukin-1 alpha, interleukin-1 beta and interleukin-6. Mol-Pharmacol 43:176-182
2. Babu JS, Kanangat S, Rouse BT (1993) Limitations and modifications of quantitative polymerase chain reaction. J Immunol Meth 165:207-216
3. Brennan FM, Field M, Chu CQ, Feldmann M, Maini RN (1991) Cytokine expression in rheumatoid arthritis. Br J Rheumatol 30:76-80
4. Chen E, Keystone EC, Fisch EN (1993) Restricted cytokine expression in rheumatoid arthritis. Ann Rheum Dis 53:485-487
5. Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156-159
6. Farahat MN, Yanni G, Poston R, Panayi GS (1993) Cytokine expression in synovial membranes of patients with rheumatoid arthritis and osteoarthritis. Ann Rheum Dis 52:870-887
7. Feldmann M, Brennan FM, Chantry D et al. (1990) Cytokine production in the arthritis joint: implications for treatment. Ann Rheum Dis 49:480-486
8. Firestein GS, Alvaro-Garcia JM, Maki R (1990) Quantitative analysis of cytokine gene expression in rheumatoid arthritis. J Immunol 144:3347-3353
9. Fritz P, Laschner W, Saal JG, Deichsel G, Tuczek HV, Wegner G (1989) Histological classification of synovitis. Zentralbl Allg Pathol Anal 135:729-741
10. Gilliland G, Perrin S, Blanchard K, Bunn HF (1990) Analysis of cytokine mRNA and DNA: detection and quantification by competitive polymerase chain reaction. Proc Natl Acad Sci USA 87:2725-2729
11. Kahle P, Saal JG, Pawelec G (1991) Cytokines in rheumatoid arthritis. Ann Rheum Dis 50:405-407
12. Kahle P, Saal JG, Schaudt K, Zacher J, Fritz P, Pawelec G (1992) Determination of cytokines in synovial fluids: correlation with diagnosis and histomorphological characteristics of synovial tissues. Ann Rheum Dis 51:731-734
13. Kanangat S, Solomon A, Rouse BT (1992) Use of quantitative polymerase chain reaction to quantitate cytokine messenger RNA molecules. Mol Immunol 29:1229-1236
14. Kolls J, Deinigner P, Cohen JC, Larson J (1993) cDNA equalization for reverse transcription-polymerase chain reaction. Anal Biochem 208:264-269
15. Laschner W, Fritz P, Rautenstrauch H, Saal HJ, Tuczek HV, Wegner G (1988) Klinische Wertigkeit der histomorphologischen Untersuchungen von Gelenkkapseln. Z Rheumatol 47:195-200
16. Melby PC, Darnell BJ, Tryon VV (1993) Quantitative measurement of human cytokine gene expression by polymerase chain reaction. J Immunol Meth 159:235-244
17. Miossec P (1992) Cytokine abnormalities in inflammatory arthritis. Baillieres Clin Rheumatol 6:373-392
18. Wallen K, Sioud M, Naivig JB, Forre O (1992) Spontaneous in vivo gene transcription of interleukin-2, interleukin-3, interleukin-4, interleukin-6, interferon-gamma, interleukin-2 receptor (CD25) and proto-oncogene c-myc by rheumatoid synovial T lymphocytes. Scan J Immunol 36:865-873
19. Saal JG, Steidle M, Einsele H, Müller CA, Fritz P, Zacher J (1992) Persistence of B19 parvovirus in synovial membranes of patients with rheumatoid arthritis. Rheumatol Int 12:147-151
20. Sewell KL, Trentham DE (1993) Pathogenesis of rheumatoid arthritis. Lancet 341:283-290
21. Siebert PD (1993) Quantitative RT-PCR. Methods and applications, book 3. Clontech Laboratories
22. Sieben PD, Larrick JW (1992) Competitive PCR. Nature 359:557-558
23. Siebert PD, Larrick W (1993) PCR Mimics: competitive DNA fragments for use as internal standard in quantitative PCR. Biotechniques 14:244-249
24. Simon AK, Seipelt E, Sieper J (1994) Divergent T-cell cytokine patterns in inflammatory arthritis. Proc Natl Acad Sci USA 91:8562-8566
25. Wang AM, Dolye MV, Mark DF (1989) Quantification of RNA by polymerase chain reaction. Proc Natl Acad Sci USA 86:9717-9721
26. Zvaifler NJ, Boyle B, Firestein GS (1994) Early synovitis-synoviocytes and mononuclear cells. Semin Arthritis Rheumat 23:11-16
27. Zvaifler NJ, Firestein GS (1994) Pannus and pannocytes. Arthritis Rheum 37:783-789