The phospholipid analogue hexadecylphosphocholine (HePC) inhibits proliferation of keloid fibroblasts in vitro and modulates their fibronectin and integrin synthesis

Archives of Dermatological Research

Volumn 289, Issue 3, 1997, Pages 164-169

  Blume Peytavi, U ^a   Geilen, C C ^a   Sommer, Ch ^a   Almond Roesler, B ^a   Orfanos, C E ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

Antiproliferative drugs; Fibronectin; Hexadecylphosphocholine; Hypertrophic scars; Integrin; Keloid

Indexed keywords

ANTINEOPLASTIC AGENT; COLLAGEN TYPE 1; FIBRONECTIN; INTEGRIN; MILTEFOSINE; PHOSPHATIDYLCHOLINE; PROTEIN SUBUNIT;

ARTICLE; CELL CULTURE; CELL METABOLISM; CELL PROLIFERATION; CONTROLLED STUDY; CULTURE MEDIUM; FIBROBLAST; HUMAN; HUMAN CELL; HYPERTROPHIC SCAR; KELOID; MALE; MESENCHYME CELL; NORMAL HUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS; TUMOR CELL;

CELL DIVISION; CELLS, CULTURED; COLLAGEN; EXTRACELLULAR MATRIX; FIBROBLASTS; FIBRONECTINS; HUMANS; INTEGRINS; KELOID; MALE; PHOSPHATIDYLCHOLINES; PHOSPHORYLCHOLINE; REFERENCE VALUES;

EID: 0031020661     PISSN: 03403696     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004030050173     Document Type: Article

References (34)

1. Nemeth A (1993) Keloids und hypertrophic scars. J Dermatol Surg Oncol 19:738-746
2. Berman B, Bieley H (1995) Keloids. J Am Acad Dermatol 33:117-123
3. Kischer CW, Shetlar MR, Chvapil (1982) Hypertrophic scars and keloids: a review and new concept concerning their origin. Scanning Electron Microsc 4:1699-1713
4. Abergel RP, Pizurro D, Meeker A, Lask L, Matsuoka LY, Minor RR, Chu ML, Ditto J (1985) Biochemical composition of the connective tissue in keloids and analysis of collagen metabolism in keloid fibroblast cultures. J Invest Dermatol 84: 384-390
5. Kischer CW, Hendrix JC (1983) Fibronectin (FN) in hypertrophic scars and keloids. Cell Tissue Res 231:29-37
6. Babu M, Diegelmann R, Oliver N (1989) Fibronectin is over-produced by keloid fibroblasts during abnormal wound healing. Mol Cell Biol 9:1642-1650
7. Muschiol C, Berger MR, Schuler B, Scherf HR, Garzon FT, Zeller WJ, linger C, Eibl HJ, Schmähl D (1988) Alkylphosphocholines: toxicity and anticancer properties. Lipids 22:930-934
8. Hilgard P, Stekar J, Voegeli R, Engel J, Schumacher W, Eibl H, Unger C, Berger MR (1988) Characterization of the antitumor activity of hexadecylphosphocholine (D 18506). Eur J Clin Oncol 24:1437-1461
9. Unger C, Eibl H, Breiser A, von Heyden HW, Engel J, Hilgard P, Sindermann H, Peukert M, Nagel GA (1988) Hexadecylphosphocholine (D 18506) in the topical treatment of skin metastases: a phase-I trial. Onkologie 11:295-296
10. Hilgard P, Harlemann JH, Voegeli R, Maurer HR, Echanti D, Unger C (1989) The antineoplastic activity of hexadecylphosphocholine (HePC) is associated with tumor cell differentiation. Proc Am Assoc Cancer Res 30:2310
11. Dummer R, Roger J, Vogt T, Becker J, Hefner H, Sindermann H, Burg G (1992) Topical application of hexadecylphophocholine in patients with cutaneous lymphomas. Prog Exp Tumor Res 34:160-169
12. Detmar M, Geilen CC, Wieder T, Orfanos CE, Reutter W (1994) Phospholipid analogue hexadecylphosphocholine inhibits proliferation and phosphatidylcholine biosynthesis of human epidermal keratinocytes in vitro. J Invest Dermatol 102: 490-494
13. Stadler R, Detmar M, Stephanek K, Bangemann C, Orfanos CE (1989) A rapid fluorometric assay for the determination of keratinocyte proliferation in vitro. J Invest Dermatol 93:532-534
14. 14C]-choline. J Labelled Comp Radiopharm 31:1071-1076
15. Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911-917
16. Touchstone JC, Chen JC, Beaver KM (1980) Improved separation of phospholipids in thin layer chromatography. Lipids 15: 61-62
17. Krasagakis K, Garbe C, Krüger S, Orfanos CE (1991) Effects of interferons on cultured human melanocytes in vitro: interferon-beta but not -alpha or -gamma inhibits proliferation and all interferons significantly modulate the cell phenotype. J Invest Dermatol 97:364-372
18. Schön MP, Schön M, Klein CE, Blume U, Bisson S, Orfanos CE (1994) Carcinoma-associated 38 kD membrane glycoprotein MH 99/KS 1/4 is related to proliferation and age of transformed epithelial cell lines. J Invest Dermatol 102:987-991
19. Schön MP, Schön M, Mattes MJ, Stein R, Weber L, Alberti S, Klein CE (1993) Biochemical and immunological characterization of the human carcinoma-associated antigen MH 99/KS 1/4. Int J Cancer 55:988-995
20. Adams LW, Priestley GC (1988) Contraction of collagen lattices by skin fibroblasts: drug-induced changes. Arch Dermatol Res 280:114-118
21. Miller EJ, Rhodes RK (1982) Preparation and characterization of the different types of collagen. Methods Enzymol 82:36-64
22. 1 (VLA-2) mediates reorganization and contraction of collagen matrices by human cells. Cell 67:403-410
23. 1 is upregulated in fibroblasts and highly aggresive melanoma cells in three-dimensional collagen lattices and mediates the reorganization of collagen I fibrils. J Cell Biol 115:1427-1436
24. Nakaoka H, Miyauchi S, Miki Y (1995) Proliferating activity of dermal fibroblasts in keloids and hypertrophic scars. Acta Derm Venereol 75:102-104
25. Kikuchi K, Kadono T, Takehara K (1995) Effects of various growth factors and histamine on cultured keloid fibroblasts. Dermatology 190:4-8
26. Martin P, Hopkinson-Wooley J, McCluskey J (1992) Growth factors and cutaneous wound repair. Prog Growth Factor Res 4:25-44
27. Albelda S, Clayton Buck (1990) Integrins and other cell adhesion molecules. FASEB J 4:2868-2880
28. Hynes RO (1992) Integrins: versatility, modulation, and signaling in cell adhesion. Cell 69:11-25
29. Iwasaki T, Chen JD, Kim JP, Wynn KC, Woodley DT (1994) Dibutyryl cyclic AMP modulates keratinocyte migration without alteration of integrin expression. J Invest Dermatol 102:891-897
30. Geilen CC, Wieder T, Reutter W (1992) Hexadecylphosphocholine inhibits translocation of CTP:Choline-phosphate cytidylyltransferase in Madin-Darby canine kidney cells. J Biol Chem 267:6719-6724
31. Geilen CC, Haase A, Buchner K, Wieder T, Hucho F, Reutter W (1991) The phospholipid analogue, hexadecylphosphocholine, inhibits protein kinase C in vitro and antagonizes phorbol ester stimulated cell proliferation. Eur J Cancer 27:1650-1653
32. Geilen CC, Haase A, Wieder T, Arndt DT, Zeisig R, Reutter W (1994) Phospholipid analogues: side chain- and polar head group-dependent effects on phosphatidylcholine biosynthesis. J Lipid Res 35:625-632
33. Geilen CC, Wieder T, Haase A, Reutter W, Morré DM, Morré DJ (1994) Uptake, subcellular distribution and metabolism of the phospholipid analogue hexadecylphosphocholine in MDCK cells. Biochim Biophys Acta 1211:14-22
34. Boggs KP, Rock CO, Jackowski S (1995) Lysophosphatidylcholine and 1-O-octadecyl-2-O-methyl-rac-glycero-3-phosphocholine inhibit the CDP-choline pathway of phosphatidylcholine synthesis at the CTP:phosphocholine cytidyltransferase step. J Biol Chem 270:7757-7764