Alanine-scanning mutagenesis of protein phosphatase type 1 in the yeast Saccharomyces cerevisiae

Genetics

Volumn 145, Issue 3, 1997, Pages 615-626

  Baker, Stefanie H ^a   Frederick, Debra L ^b   Bloecher, Andrew ^c   Tatchell, Kelly ^d  

^a NORTH CAROLINA STATE UNIVERSITY   (United States)

^b LOUISIANA STATE UNIVERSITY   (United States)

^c LOUISIANA STATE UNIV MEDICAL CENTER   (United States)

^d CLEMSON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; BINDING PROTEIN; DEOXYGLUCOSE; GLUCOSE; GLYCOGEN; MUTANT PROTEIN; PHOSPHOPROTEIN PHOSPHATASE 1;

ALLELE; ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CELL CYCLE; CODON; CONTROLLED STUDY; FLOW CYTOMETRY; FUNGUS SPORE; GENE MUTATION; GLYCOGEN SYNTHESIS; IMMUNOFLUORESCENCE MICROSCOPY; LETHAL MUTANT; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SPOROGENESIS;

ALANINE; ALLELES; FUNGAL PROTEINS; GENES, LETHAL; GLUCOSE; GLYCOGEN; MUTAGENESIS; PHOSPHOPROTEIN PHOSPHATASE; SACCHAROMYCES CEREVISIAE; SPORES, FUNGAL;

FUNGI; SACCHAROMYCES CEREVISIAE;

EID: 0031019156     PISSN: 00166731     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (58)

1. BARTON, G. J., P. T. W. COHEN and D. BARFORD, 1994 Conservation analysis and structure prediction of the protein serine/threonine phosphatases. Eur.J. Biochem. 220: 225-237.
2. BLACK, S., P. D. ANDREWS, A. A. SNEDDON and M. J. R. STARK, 1995 A regulated MET3-GLC7 gene fusion provides evidence of a mitotic role for Saccharomyces cerevisiae. Yeast 11: 747-759.
3. BOLLEN, M., and W. STALMANS, 1992 The structure, role, and regulation of type 1 protein phosphatases. Crit. Rev. Biochem. Mol. Biol. 27: 227-281.
4. BREUDER, T., C. S. HEMENWAY, N. R. MOWA, M. E. CARDENAS and J. HEITNL\N, 1994 Calcineurin is essential in cyclosporin A-and FK506-sensiuve yeast strains. Proc. Natl. Acad. Sei. USA 91:5372-5376.
5. CANNON, J. F., J. R. PRINGLE, A. FIECHTER and M. KHALIL, 1994 Characterization of glycogen-deficient glc mutants of Saccharomyces cerevisiae. Genetics 136: 485-503.
6. CANNON, J. F., and K. TATCHELL, 1987 Characterization ofSaccharo-myces cerevisiae genes encoding subunits of cyclic AMP-dependent protein kinase. Mol. Cell. Biol. 7: 2653-2663.
7. CLARK, S. W., and D. I. MEYER, 1994 ACT3: a putative centractin homologue in S. cerevisiae is-required for proper orientation of the mitotic spindle. J. Cell. Biol. 127: 129-138.
8. COHEN, P., and P. T. W. COHEN, 1989 Protein phosphatases come of age. J. Biol. Chem. 264: 21435-21438.
9. CUNNINGHAM, B. C., and J. A. WELLS, 1989 High-resolution epitope mapping of hGH-receptor interactions by alanine-scanning mutagenesis. Science 244: 1081-1085.
10. DENT, P., A. LAVOINNE, S. NAKIELNY, F. B. CAUD\VELL, P. WATT et al., 1990 The molecular mechanism by which insulin stimulates glycogen synthesis in mammalian skeletal muscle. Nature 348: 302-308.
11. DOHADWALA, M., E. F. DA CRUZ E SILVA, F. L. HALL, R. T. WILLIAMS, D. A. CARBONARO-HALL et al., 1994 Phosphorylation and inactivation of protein phosphatase 1 by cyclin-dependent kinases. Proc. Natl. Acad. Sei. USA 91: 6408-6412.
12. EGLOFF, M. P., P. T. COHEN, P. REINEMER and D. BARFORD, 1995 Crystal structure of the catalytic subunit of human protein phosphatase 1 and its complex with tungstate. J. Mol. Biol. 254: 942-959.
13. ENTIAN, K. D., and F. K. ZIMMERMANN, 1980 Glycolytic enzymes and intermediates in carbon catabolite repression mutants ol Saccharomyces cerevisiae. Mol. Gen. Genet. 177: 345-350.
14. ESHEL, D., L. A. URRESTARAZU, S. VISSERS, J.-C. JAUNIAUX, J. C. VAN VLIET-REEDIJK et al., 1993 Cytoplasmic dynein is required for normal nuclear segregation in yeast. Proc. Natl. Acad. Sei. USA 90: 11172-11176.
15. FENG, Z., S. E. WILSON, Z.-Y. PENG, K. K. SCHLENDER, E. M. REIMANN et al., 1991 The yeast GLC7gene required for glycogen accumulation encodes a type 1 protein phosphatase. J. Biol. Chem. 266: 23796-23801.
16. FRANÇOIS, J., P. ERASO and C. GANCEDO, 1987 Changes in the concentration of cAMP, fructose 2, 6-bisphosphate and related metabolites and enzymes in Saccharomyces cerevisiae during growth on glucose. Eur. J. Biochem. 164: 369-373.
17. FRANÇOIS, J. M., S. THOMPSON-JAEGER, J. SKROCH, U. ZELLENKA, W. SPEVAK et al., 1992 GAC1 may encode a regulatory subunit for protein phosphatase type 1 in Saccharomyces cerevisiae. EMBO J. 11: 87-96.
18. FREDERICK, D. L., and K. TATCHELL, 1996 The EEG2 gene of Saccharomyces cerevisiae encodes a type 1 protein phosphatase-binding protein that functions with Reg1p and the Snf1p protein kinase to regulate growth. Mol. Cell. Biol. 16: 2922-2931.
19. GIBES, C. S., and M. J. ZOLLER, 1991 Rational scanning mutagenesis of a protein kinase identifies functional regions involved in catalysis and substrate interactions. J. Biol. Chem. 266: 8923-8931.
20. GIETZ, D., A. ST. JEAN, R. A. WOODS and R. H. SCHIESTL, 1992 Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res. 20: 1425.
21. GOLDBERG, J., H. B. HUANG, Y. G. KWON, P. GREENGARD, A. C. NAIRN et al, 1995 Three-dimensional structure of the catalytic subunit of protein serine/threonine phosphatase-1. Nature 376: 745-753.
22. HARDY, T., and P. ROACH, 1993 Control of yeast glycogen synthase-2 by COOH-terminal phosphorylation. J. Biol. Chem. 268: 23799-23805.
23. HISAMOTO, N., D. L. FREDERICK, K. SUGIMOTO, K. TATCHELL and K. MATSUMOTO, 1995 The EGP1 gene may be a positive regulator of protein phosphatase type 1 in the growth control of Saccharomyces cerevisiae. Mol. Cell. Biol. 15: 3767-3776.
24. HKAMOTO, N., K. SUGIMOTO and K. MATSUMOTO, 1994 The Glc7 type 1 protein phosphatase of Saccharomyces cerevisiae is required for cell cycle progression in G2/M. Mol. Cell. Biol. 14: 3158-3165.
25. HOLTZMAN, D. A., S. YANG and D. G. DRUBIN, 1993 Synthetic-lethal interactions identify two novel genes, SLA1 and SLA2, that control membrane cytoskeleton assembly in Saccharomyces cerevisiae. J. Cell Biol. 122: 635-644.
26. LIi, Y.-Y., E. YEH, T. HAYS and K. BLOOM, 1993 Disruption of mitotic spindle orientation in a yeast dynein mutant. Proc. Nad. Acad. Sei. USA 90: 10096-10100.
27. MACKELVIE, S. H., P. D. ANDREWS and M. J. R. STARK, 1995 The Saccharomyces cerevisiae gene SDS22 encodes a potential regulator of the mitotic function of yeast type 1 protein phosphatase. Mol. Cell. Biol. 15: 3777-3785.
28. MAXIATIS, T., J. SAMBROOK and E. F. FRTTSCH, 1989 Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
29. MATSUMOTO, K., T. YOSHIMATSU and Y. OSHIMA, 1983 Recessive mutations conferring resistance to carbon catabolite repression of galactokinase synthesis in Sacchawmyces cerevisiae. J. Bacteriol. 153: 1405-1414.
30. MATSUURA, A., and Y. ANRAKU, 1994 Genetic interaction between the Ras-cAMP pathway and the Dis2s1/Glc7 protein phosphatase in Saccharomyces cerevisiae. Mol. Gen. Genet. 242: 257-262.
31. MCCUSKER, J. H., D. S. PERLIN and J. E. HABER, 1987 Pleiotropic plasma membrane ATPase mutations of Sacchammyces cerevisiae. Mol. Cell. Biol. 7: 4082-4088.
32. MCMILLAN, J. N., and K. TATCHELL, 1994 The JNM1 gene in the yeast Sacchawmyces cerevisae is required for nuclear migration and spindle orientation during the mitotic cell cycle. J. Cell Biol. 125: 143-158.
33. MENDOZA, I., F. RUBIO, A. RODRIGUEZ-NAVARRO and J. M. PARDO, 1994 The protein phosphatase calcineurin is essential for NaCl tolerance of Saccharomyces cerevisiae. J. Biol. Chem. 269: 8792-8796.
34. MUHUA, L., T. S. KARPOVA and J. A. COOPER, 1994 A yeast actin-related protein homologous to that found in vertebrate dynactin complex is required for spindle orientation and nuclear migration. Cell 78: 669-679.
35. NAKAMURA, T., Y. LIU, D. HIRATA, H. NAMBA, S. HARADA a al., 1993 Protein phosphatase type 2B (calcineurin)-mediated, FK506-sensitive regulation of intracellular ions in yeast is an important determinant for adaptation to high salt stress conditions. EMBO J. 12: 4063-4071.
36. NEIGEBORN, L., and M. CARLSON, 1987 Mutations causing constitutive invertase synthesis in yeast: genetic interactions with jn/mutations. Genetics 115: 247-253.
37. NELSON, K. K., M. HOLMER and S. K. LEMMON, 1996 SCD5, a suppressor of clathrin deficiency, encodes a novel protein with a late secretory function in yeast. Mol. Biol. Cell 7: 245-260.
38. NOVICK, P., and D. BOTSTEIN, 1985 Phenotypic analysis of temperature-sensitive yeast actin mutants. Cell 40: 405-416.
39. + gene required for chromosome disjoining encodes one of two putative type 1 protein phosphatases. Cell 57: 997-1007.
40. PALMER, R. E., D. S. SULLIVAN, T. HUFFAKER and D. KOSHLAND, 1992 Role of astral microtubules and actin in spindle orientation and migration in the budding yeast, Saccharomyces cerevisiae. J. Cell Biol. 119:583-593.
41. POSAS, F., M. CAMPS and J. ARINO, 1995 The PPZ protein phosphatases are important determinants of salt tolerance in yeast cells. J. Biol. Chem. 270: 13036-13041.
42. REIJO, R. A., E. M. COOPER, G.J. BEAGLE and T. C. HUFFAKER, 1994 Systematic mutational analysis of the yeast beta-tubulin gene. Mol. Biol. Cell. 5: 29-43.
43. ROCKMILL, B., and G. S. ROEDER, 1988 RED1: a yeast gene required for the segregation of chromosomes during the reductional division of meiosis. Proc. Natl. Acad. Sei. USA 85: 6057-6061.
44. ROCKMILL, B., and G: S. ROEDER, 1990 Meiosis in asynaptic yeast. Genetics 126: 563-574.
45. ROSE, M. D., F. WINSTON and P. HIETER, 1990 Methods in Yeast Genetics. A Laboratory Course Manual. Cold Spring Harbor Laboratory Press, Plainview, NY.
46. SCHULLER, C., J. L. BREWSTER, M. R. ALEXANDER, M. C. GUSTIN and H. Ruis, 1994 The HOG pathway controls osmotic regulation of transcription via the stress response element (STRE) of the Saccharomyces cerevisiae CTT1 gene. EMBO J. 13: 4382-4389.
47. SHENOLIKAR, S., 1994 Protein serine/threonine phosphatasesnew avenues for cell regulation. Annu. Rev. Cell Biol. 10: 55-86.
48. SINCH, A., 1977 Nonsense suppressors of yeast cause osmotic-sensitive growth. Proc. Natl. Acad. Sei. USA 74: 305-309.
49. STONE, E. M., H. YAMANO, N. KINOSHITA and M. YANAGIDA, 1993 Mitotic regulation of protein phosphatases by the fission yeast sds22 protein. Curr. Biol. 3: 13-26.
50. STUART, J. S., D. L. FREDERICK, C. M. VARNER and K. TATCIIELL, 1994 The mutant type 1 protein phosphatase encoded by glc7-l from Saccharomyces cerevisiae fails to interact productively with the GAC1-encoded regulatory subunit. Mol. Cell. Biol. 14: 896-905.
51. SUTTON, A., F. LIN, M. J. F. SARABIA and K. T. ARNDT, 1991 The SIT4 protein phosphatase is required in late GI for progression into S phase. Cold Spring Harbor Symp. Quant. Biol. 56: 75-81.
52. TU, J., and M. CARLSON, 1994 The GLC7 type 1 protein phosphatase is required for glucose repression in Saccharomyces cerevisiae. Mol. Cell. Biol. 14: 6789-6796.
53. TU, J., and M. CARLSON, 1995 REGI binds to protein phosphatase type 1 and regulates glucose repression in Saccharomyces cerevisiae. EMBOJ. 14: 5939-5946.
54. Tu, J., W. SONG and M. CARLSON, 1996 Protein phosphatase type 1 interacts with proteins required for meiosis and other cellular processes in Saccharomyces cerevisiae. Mol. Cell. Biol. 16: 4199-4206.
55. TUNG, H. Y., W. WANG and C. S. CHAN, 1995 Regulation of chromosome segregation by Glc8p, a structural homolog of mammalian inhibitor 2 that functions as both an activator and an inhibitor of yeast protein phosphatase 1. Mol. Cell. Biol. 15: 6064-6074.
56. WERTMAN, K. F., D. G. DRUBIN and D. BOTSTEIN, 1992 Systematic mutational analysis! of the yeast ACT1 gene. Genetics 132: 337-350.
57. WILSON, I. A., H. L. NIMAN, R. A. HOUGHTEN, A. R. CHERENSON, M. L. CONNOLLY et al., 1984 The structure of an antigenic determinant in a protein. Cell 37: 767-778.
58. YAMANO, H., K. ISHII and M. YANAGIDA, 1994 Phosphorylation of dis2 protein phosphatase at the C-terminal cdc2 consensus and its potential role in cell cycle regulation. EMBO J. 13: 5310-5318.