Role of conserved residues in hydrophilic loop 8-9 of the lactose permease

Journal of Bacteriology

Volumn 179, Issue 3, 1997, Pages 735-741

  Pazdernik, Nanette J ^a   Jessen Marshall, Amy E ^a   Brooker, Robert J ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; ASPARAGINE; ASPARTIC ACID; GLYCINE; LACTOSE; LACTOSE PERMEASE; MEMBRANE PROTEIN; PEPTIDE; PROLINE; THREONINE;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL MEMBRANE; CONFORMATIONAL TRANSITION; IMMUNOBLOTTING; MEMBRANE TRANSPORT; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

EID: 0031018881     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.3.735-741.1997     Document Type: Article

References (40)

1. Birnboim, H. C., and J. Doly. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7:1513-1523.
2. Brooker, R. J. 1990. The lactose permease of Escherichia coli. Res. Microbiol. 141:309-316.
3. 319 permeases facilitate proton uniport and sugar uniport. J. Biol. Chem. 266:4131-4138.
4. Brooker, R. J., and T. H. Wilson. 1985. Isolation and nucleotide sequencing of lactose carrier mutants that transport maltose. Proc. Natl. Acad. Sci. USA 82:3959-3963.
5. Büchel, D. E., B. Gronenborn, and B. Müller-Hill. 1980. Sequence of the lactose permease gene. Nature 283:541-545.
6. Calemia, J., and C. Manoil. 1990. lac permease of Escherichia coli: topology and sequence elements promoting membrane insertion. Proc. Natl. Acad. Sci. USA 87:4937-4941.
7. Chang, A. C. Y., and S. N. Cohen. 1978. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from P15A cryptic miniplasmid. J. Bacteriol. 134:1141-1156.
8. Crane, R. K. 1977. The gradient hypothesis and other models of carrier-mediated active transport. Rev. Physiol. Biochem. Pharmacol. 78:99-159.
9. Foster, D. L., M. Boublik, and H. R. Kaback. 1983. Structure of the lac carrier protein of Escherichia coli. J. Biol. Chem. 258:31-34.
10. Franco, P. J., and R. J. Brooker. 1994. Functional roles of Glu-269 and Glu-325 within the lactose permease of Escherichia coli. J. Biol. Chem. 269:7379-7386.
11. Goswitz, V. C., and R. J. Brouker. 1995. Structural features of the uniporter/ symporter/antiporter superfamily. Protein Sci. 4:543-537.
12. Griffith, J. K., M. E. Baker, D. A. Rouch, M. G. P. Page, R. A. Skurray, I. T. Paulsen, K. F. Chater, S. A. Baldwin, and P. J. F. Henderson. 1992. Membrane transport proteins: implications of sequence comparisons. Curr. Opin. Cell Biol. 4:684-695.
13. Henderson, P. J. F. 1990. Proton-linked sugar transport systems in bacteria. J. Bioenerg. Biomembr. 22:525-569.
14. Jessen-Marshall, A. E., N. J. Paul, and R. J. Brooker. 1995. The conserved motif, GXXX(D/E)(R/K)XG[X](R/K)(R/K), in hydrophilic loop 2/3 of the lactose permease. J. Biol. Chem. 270:16251-16257.
15. Jung, K., H. Jung, P. Colarcurio, and H. R. Kaback. 1995. Role of glycine residues in the structure and function of lactose permease, an Escherichia coli membrane transport protein. Biochemistry 34:1030-1039.
16. Kaczorowski, G. J., and H. R. Kaback. 1979. Mechanism of lactose translocation in membrane vesicles from Escherichia coli. 1. Effect of pH on efflux, exchange and counterflow. Biochemistry 18:3691-3697.
17. H+. Biochemistry 18:3697-3704.
18. King, S. C., C. L. Hansen, and T. H. Wilson. 1991. The interaction between aspartic acid 237 and lysine 358 in the lactose carrier of Escherichia coli. Biochim. Biophys. Acta 1062:177-186.
19. Kraft, R., J. Tardiff, K. S. Drauter, and L. A. Leinwand. 1988. Using miniprep plasmid DNA for sequencing double stranded templates with sequenase. BioTechniques 6:544-547.
20. Kunkel, T. A., J. D. Roberts, and R. A. Zabour. 1987. Rapid and efficient site-specific mulageiiesis without phenotypic selection. Methods Enzymol. 154:367-382.
21. Maiden, M. C. J., E. O. Davis, S. A. Baldwin, D. C. M. Moore, and P. J. F. Henderson. 1987. Mammalian and bacterial sugar transport proteins are homologous. Nature 325:641-643.
22. Mandel, M., and A. Higa. 1970. Calcium-dependent bacteriophage DNA infection. J. Mol. Biol. 63:154-162.
23. Marger, M. D., and M. H. Saier, Jr. 1993. A major superfamily of transmembrane facilitators that catalyze uniport, symport, and antiport. Trends Biochem. Sci. 18:13-20.
24. McKenna, E., D. Hardy, and H. R. Kaback. 1992. Insertional mutagenesis of hydrophilic domains in the lactose permease of Escherichia coli. Proc. Natl. Acad. Sci. USA 89:11954-11958.
25. Miller, J. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
26. Mitchell, P. 1963. Molecule, group, and electron translocation through natural membranes. Biochem. Soc. Symp. 22:142-168.
27. Newman, M. J., and T. H. Wilson. 1980. Stabilization and reconstitution of the lactose transport system of Escherichia coli. J. Biol. Chem. 255:10583-10586.
28. Rickenberg, H. V., G. Cohen, G. Buttin, and J. Monod. 1956. La galactosidepermease d'Escherichia coli. Ann. Inst. Pasteur (Paris) 91:829-857.
29. + symport. Res. Microbiol. 141:290-308.
30. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Aead. Sci. USA 74:5463-5467.
31. Segal, I. 1975. Enzyme kinetics. Wiley Interscience, New York, N.Y.
32. Teather, R. M., J. Bramhall, I. Riede, J. K. Wright, M. Fürst, G. Aichele, U. Wilhelm, and P. Overath. 1980. Lactose carrier protein of Escherichia coli: structure and expression of plasmids carrying the Y gene of the lac operon. Eur. J. Biochem. 108:223-231.
33. Teather, R. M., B. Müller-Hill, U. Abrutsch, G. Aichele, and P. Overath. 1978. Amplification of the lactose carrier protein in Escherichia coli using a plasmid vector. Mol. Gen. Genet. 159:239-248.
34. + symport across the plasma membrane of Escherichia coli. Biochem. J. 132:587-592.
35. + antiporter of Escherichia coli. J. Biochem. 115:958-964.
36. + antiporter of Escherichia coli encoded by transposon Tn10: the structural resemblance and functional difference in the role of the duplicated sequence motif between hydrophobia segments 2 and 3 and segments 8 and 9. J. Biol. Chem. 268:6496-6504.
37. + antiporter of Escherichia coli encoded by a transposon, Tn10. the role of the conserved dipeptide, Ser65-Asp66, in tetracycline transport. J. Biol. Chem. 265:15525-15530.
38. + antiporter of Escherichia coli encoded by transposon Tn10: the role of a conserved sequence motif, GXXXXRXGRR, in a putative cytoplasmic loop between helices 2 and 3. J. Biol. Chem. 267:19155-19162.
39. Yanisch-Perron, C., H. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene (Amsterdam) 33:103-119.
40. Zoller, M. J., and M. Smith. 1983. Oligonucleolide-directed mutagenesis of DNA fragments cloned into M13 vectors. Methods Enzymol. 100:468-500.