A sensitive reporter gene system using bacterial luciferase based on a series of plasmid cloning vectors compatible with derivatives of pBR322

Gene

Volumn 186, Issue 2, 1997, Pages 197-200

  Manen, Danielle ^a,b   Pougeon, Maryse ^a   Damay, Pascal ^a   Geiselmann, Johannes ^a  

^a UNIVERSITY OF GENEVA   (Switzerland)

^b GENEVA UNIVERSITY HOSPITALS   (Switzerland)

Author keywords

Escherichia coli; Luminosity; Origin of replication; Promoter strength; pSC101

Indexed keywords

ALDEHYDE; BACTERIAL ENZYME; LUCIFERASE; SPECTINOMYCIN; STREPTOMYCIN;

ARTICLE; CLONING VECTOR; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; REPORTER GENE;

BASE SEQUENCE; CLONING, MOLECULAR; ENTEROBACTERIACEAE; ESCHERICHIA COLI; GENES, BACTERIAL; GENES, REPORTER; GENETIC VECTORS; LUCIFERASES; MOLECULAR SEQUENCE DATA; PLASMIDS; PROMOTER REGIONS (GENETICS); RESTRICTION MAPPING;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0031018617     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(96)00702-0     Document Type: Article

References (7)

1. Churchward, G., Belin, D. and Nagamine, Y. (1984) ApSC101 derived plasmid which shows no sequence homology to other commonly used cloning vectors. Gene 31, 285-290.
2. Déthiollaz, S., Patrick Eichenberger, P. and Geiselmann, J. (1996) Influence of DNA geometry on transcriptional activation in Escherichia coli. EMBO J. 15, 5449-5458.
3. Fernández-Piñas, F. and Wolk, P.C. (1994) Expression of luxCD-E in Anabaena sp. can replace the use of exogenous aldehyde for in vivo localization of transcription by luxAB. Gene 150, 169-174.
4. Meighen, E.A. (1993) Bacterial bioluminescence: organization, regulation, and application of lux genes. FASEB J. 7, 1016-1022.
5. Raibaud, O., Vidal-Ingigliardi, D. and Kolb, A. (1991) Genetic studies on the promoter of malT, the gene that encodes the activator of the Escherichia coli maltose regulon. Res. Microbiol. 142, 937-942.
6. Xia, G.-X., Manen, D., Goebel, T., Linder, P., Churchward, G. and Caro, L. (1991) A copy number mutant of plasmid pSC101. Mol. Microbiol. 5, 631-640.
7. Yanish-Perron, C., Vieira, J., and J. Messing, J. (1983) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33, 103-119.