GluR2 glutamate receptor subunit flip and flop isoforms are decreased in the hippocampal formation in schizophrenia: A reverse transcriptase-polymerase chain reaction (RT-PCR) study

Molecular Brain Research

Volumn 44, Issue 1, 1997, Pages 92-98

  Eastwood, Sharon L ^a,b   Burnet, Philip W J ^a,b   Harrison, Paul J ^a,b  

^a WARNEFORD HOSPITAL   (United Kingdom)

^b RADCLIFFE INFIRMARY   (United Kingdom)

Author keywords

Agonal state; Alternative splicing; AMPA receptor; Human brain; mRNA; Non NMDA receptor; Schizophrenia

Indexed keywords

AMPA RECEPTOR; BRAIN RECEPTOR; CYCLOPHILIN; GLUTAMATE RECEPTOR; MESSENGER RNA; RECEPTOR SUBUNIT;

ADULT; AGE; AGED; ALTERNATIVE RNA SPLICING; ARTICLE; AUTOPSY; CLINICAL ARTICLE; CONTROLLED STUDY; DESENSITIZATION; FEMALE; HIPPOCAMPUS; HISTOCHEMISTRY; HUMAN; HUMAN CELL; IN SITU HYBRIDIZATION; MALE; PH; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCHIZOPHRENIA; SIGNAL TRANSDUCTION;

ADULT; AGED; FEMALE; HIPPOCAMPUS; HUMANS; MALE; MIDDLE AGED; POLYMERASE CHAIN REACTION; RECEPTORS, AMPA; RECEPTORS, GLUTAMATE; SCHIZOPHRENIA;

EID: 0031017606     PISSN: 0169328X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0169-328X(96)00195-7     Document Type: Article

References (41)

1. Akbarian, S., Smith, M.A. and Jones, E.G., Editing for an AMPA receptor subunit mRNA in prefrontal cortex and striatum in Alzheimer's disease, Huntington's disease and schizophrenia, Brain Res., 699 (1995) 297-304.
2. Barton, A.J.L., Pearson, R.C.A., Najlerahim, A. and Harrison, P.J., Pre- and post-mortem influences on brain RNA, J. Neurochem., 61 (1993) 1-11.
3. Boulter, I., Hollman, M., O'Shea-Greenfield, A., Hartley, M., Deneris, E., Maron, C. and Heinemann, S., Molecular cloning and functional expression of glutamate receptor genes, Science, 249 (1990) 1033-1036.
4. 2A receptor mRNAs in human hippocampus using a reverse transcriptase-polymerase chain reaction (RT-PCR) technique and their correlation with binding site densities and age, Neurosci. Lett., 178 (1994) 85-89.
5. Chomczynski, P. and Sacchi, N., A single step method for the isolation of RNA using the acid-phenol-chloroform method, Anal. Biochem., 162 (1987) 156-159.
6. Dukas, K., Sarfati, P., Vayesse, N. and Pradayrol, L., Quantitation of changes in the expression of multiple genes by simultaneous polymerase chain reaction, Anal. Biochem., 215 (1993) 66-72.
7. Eastwood, S.L. and Harrison, P.J., Decreased synaptophysin in the medial temporal lobe in schizophrenia demonstrated using immunoautoradiography, Neuroscience, 69 (1995) 339-343.
8. Eastwood, S.L., Burnet, P.W.J., Beckwith, J., Kerwin, R.W. and Harrison, P.J., AMPA glutamate receptors and their flip and flop mRNAs in human hippocampus, NeuroReport, 5 (1994) 1325-1328.
9. Eastwood, S.L., Story, P., Burnet, P.W.J., Heath, P. and Harrison, P.J., Differential changes in glutamate receptor subunit messenger RNAs in rat brain after haloperidol treatment, J. Psychopharmacol., 8 (1994) 196-203.
10. Eastwood, S.L., McDonald, B., Burnet, P.W.J., Beckwith, J.P., Kerwin, R.W. and Harrison, P.J., Decreased expression of mRNAs encoding non-NMDA glutamate receptors GluR1 and GluR2 in medial temporal lobe neurons in schizophrenia, Mol. Brain Res., 29 (1995) 211-223.
11. Eastwood, S.L., Porter, R.H.P. and Harrison, P.J., The effect of chronic haloperidol treatment on glutamate receptor subunit (GluR1, GluR2, NR1, KA1, KA2) mRNAs and glutamate binding protein mRNA in the rat forebrain, Neurosci. Lett., 212 (1996) 163-166.
12. Eastwood, S.L., Kerwin, R.W. and Harrison, P.J., Immunoautoradiographic evidence for a loss of α-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid-preferring non-N-methyl-D-aspartate glutamate receptors (GluR1 and GluR2 subunits) within the medial temporal lobe in schizophrenia, Biol. Psychiatry, in press.
13. Etienne, P. and Baudry, M., Calcium dependent aspects of synaptic plasticity, excitatory amino acid neurotransmission, brain ageing and schizophrenia: a unifying hypothesis, Neurobiol. Aging, 8 (1987) 362-366.
14. Feinberg, A.P. and Vogelstein, B., A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity, Anal. Biochem., 132 (1983) 6-13.
15. Geiger, J.R.P., Melcher, T., Koh, D.-S., Sakmann, B., Seeburg, P.H., Jonas, P. and Monyer, H., Relative abundance of subunit mRNAs determines gating and Ca2+ permeability of AMPA receptors in principal neurons and interneurons in rat CNS, Neuron, 15 (1995) 193-204.
16. Haendler, B., Hofer-Warbinek, R. and Hofer, E., Complementary DNA for human T-cell cyclophilin, EMBO J., 6 (1995) 947-950.
17. Harrison P.J., On the neuropathology of schizophrenia and its dementia: Neurodevelopmental, neurodegenerative, or both?, Neurodegeneration, 4 (1995) 1-12.
18. Harrison, P.J., Heath, P.R., Eastwood, S.L., Burnet, P.W.J., McDonald, B. and Pearson, R.C.A., The relative importance of premortem acidosis and postmortem interval for human brain gene expression studies: selective mRNA vulnerability and a comparison of their encoded proteins, Neurosci. Lett., 200 (1995) 151-154.
19. Hollmann, M. and Heinemann, S., Cloned glutamate receptors, Annu. Rev. Neurosci., 17 (1994) 31-108.
20. 2+ permeability of KA-AMPA-gated glutamate receptor channels depends on subunit composition, Science, 252 (1991) 851-853.
21. 2+ permeability of AMPA-type glutamate receptor channels in neocortical neurons caused by differential GluR-B subunit expression, Neuron, 12 (1994) 1281-1289.
22. Kamphuis, W., De Rijk, T.C., Talamini, L.M. and Lopes da Silva, F.H., Rat hippocampal kindling induces changes in the glutamate receptor mRNA expression patterns in dentate granule neurons, Eur. J. Neurosci., 6 (1994) 1119-1127.
23. Keinänen, K., Wisden, W., Sommer, B., Werner, P., Herb, A., Verdoorn, T.A., Sakmann, B. and Seeburg, P.H., A family of AMPA-selective glutamate receptors, Science, 249 (1990) 556-560.
24. Kerwin, R.W. and Murray, R.M., A developmental perspective on the pathology and neurochemistry of the temporal lobe in schizophrenia, Schizophr. Res., 1 (1992) 1-12.
25. Köhler, M., Kornau, H.-C. and Seeburg, P.H., The organization of the gene for the functionally dominant α-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid receptor subunit GluR-B, J. Biol. Chem., 269 (1994) 17367-17370.
26. Kristensen, P., Differential expression of AMPA glutamate receptor mRNAs in the rat adrenal gland, FEBS Lett., 332 (1993) 14-18.
27. Lambolez, B., Ropert, N., Perrais, D., Rossier, J. and Hestrin, S., Correlation between kinetics and RNA splicing of α-amino-3-hydroxy-5-methylisoxazaole-4-propionic acid receptors in neocortical neurons, Proc. Natl. Acad. Sci. USA, 93 (1996) 1797-1802.
28. McLaughlin, D.P., Cheetham, M.C. and Kerwin, R.W., Expression of alternatively-spliced glutamate receptors in human hippocampus, Eur. J. Pharmacol. - Mol. Pharmacol., 244 (1993) 89-92.
29. Monyer, H., Seeburg, P.M. and Wisden, W., Glutamate-operated channels: developmentally early and mature forms arise by alternate splicing, Neuron, 6 (1991) 799-810.
30. Morris, B.J., Control of receptor sensitivity at the mRNA level, Mol. Neurobiol., 7 (1993) 189-205.
31. Olney, J.W. and Farber, N.B., Glutamate receptor dysfunction and schizophrenia, Arch. Gen. Psychiatry, 52 (1995) 998-1007.
32. Oretti, R.G., Spurlock, G., Buckland, P.R. and McGuffin, P., Lack of effect of antipsychotic and antidepressant drugs on glutamate receptor mRNA levels in rat brains, Neurosci. Lett., 177 (1994) 39-43.
33. Pollard, H., Héron, A., Moreau, J., Ben-Ari, Y. and Khrestchatisky, M., Alterations of the GluR-B AMPA receptor flip/flop expression in kainate-induced epilepsy and ischemia, Neuroscience, 57 (1993) 545-554.
34. Sambrook, J., Fritsch, E.F. and Maniatis, T. (Eds.), Analysis and cloning of eukaryotic genomic DNA. In Molecular Cloning - A Laboratory Manual, Cold Spring Harbour Laboratory, Cold Spring Harbour, New York, 1989, Vol. 2, 2nd edn., pp. 9.38-9.40.
35. Singer-Sam, J., Robinson, M.O., Bellvé, A.R., Simon, M.I. and Riggs, A.D., Measurement by quantitative PCR of changes in HPRT, PGK-1, PGK-2, MTase, and Zfy gene transcripts during mouse spermatogenesis, Nucleic Acids Res., 18 (1990) 1255-1259.
36. Sommer, B., Keinänen, K., Verdoorn, T.A., Wisden, W., Burnashev, N., Herb, A., Köhler, M., Takagi, T., Sakmann, B. and Seeburg, P.H., Flip and flop: a cell-specific functional switch in glutamate-operated channels of the CNS, Science, 249 (1990) 1580-1585.
37. Sommer, B., Köhler, M., Sprengel, R. and Seeburg, P.H., RNA editing in the brain controls a determinant of ion flow in glutamategated channels, Cell, 67 (1991) 11-19.
38. Standley, S., Tocco, G., Tourigny, M.-F., Massicotte, G., Thompson, R.F. and Baudry, M., Developmental changes in α-amino-3-hydroxy-5-methyl-4-isoxazole propionate receptor properties and expression in the rat hippocampal formation, Neuroscience, 67 (1995) 881-892.
39. Sun, W., Ferrer-Montiel, A.V. and Montal, M., Primary structure and functional expression of the AMPA/kainate subunit 2 from human brain, NeuroReport, 5 (1994) 441-444.
40. Tsai, G., Passani, L.A., Slusher, B.S., Carter, R., Baer, L., Kleinman, J. and Coyle, J.T., Abnormal excitatory neurotransmitter metabolism in schizophrenic brains, Arch. Gen. Psychiatry, 52 (1995) 829-835.
41. Weinberger D.R., Schizophrenia: from neuropathology to neurodevelopment, Lancet, 346 (1995) 552-557.