Site-specific photobiotinylation of immunoglobulins, fragments and ligh chain dimers

Journal of Immunological Methods

Volumn 201, Issue 1, 1997, Pages 77-88

  Pavlinkova, Gabriela ^a   Rajagopalan, Krishnan ^a   Muller, Sybille ^b,c   Chavan, Ashok ^d   Sievert, Gail ^d   Lou, Dingyuan ^c   O'Toole, Carol ^c   Haley, Boyd ^a,c   Kohler, Heinz ^a,c  

^a UNIVERSITY OF KENTUCKY   (United States)

^b University of Kentucky   (United States)

^c UNIVERSITY OF KENTUCKY   (United States)

^d Immpheron Incorporated   (United States)

Author keywords

antibody; photoaffinity labeling; site specific biotinylation

Indexed keywords

BIOTIN; IMMUNOGLOBULIN FV FRAGMENT; IMMUNOGLOBULIN LIGHT CHAIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ACQUIRED IMMUNE DEFICIENCY SYNDROME; ARTICLE; BIOTINYLATION; CLINICAL ARTICLE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; IMMUNOBLOTTING; MOUSE; NONHUMAN; PHOTOAFFINITY LABELING; PRIORITY JOURNAL;

ANIMALS; ANTIBODIES, MONOCLONAL; AZIDES; BIOTIN; CELL SEPARATION; FLOW CYTOMETRY; HIV ANTIBODIES; HUMANS; IMMUNOGLOBULIN FAB FRAGMENTS; IMMUNOGLOBULIN FRAGMENTS; IMMUNOGLOBULIN LIGHT CHAINS; IMMUNOGLOBULIN M; IMMUNOGLOBULINS; MICE; PHOTOCHEMISTRY; PROTEIN DENATURATION; TUMOR CELLS, CULTURED;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 0031015507     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(96)00214-1     Document Type: Article

References (16)

1. Czarnecky, J., Geahlen, R. and Haley, B. (1979) Synthesis and use of azido photoaffinity analogs of adenine and guanidine nucleotides. Methods Enzymol. 56, 642.
2. Johnson, V.G., Schlom, J. Paterson, A.J., Bennett, J., Magnani, J. and Colcher, D. (1988) Analysis of a human tumor-associated glycoprotein TAG-72, identified by a monoclonal antibody B72.3. Cancer Res. 46, 850.
3. Khatoon, S., Cambell, S.R., Haley, B. and Slevin, J.J. (1989) Aberrant GTP b-tubulin interactions in Alzheimer's disease. Ann. Neurol. 26, 215.
4. Levy, S, Medel, E. and Kon, S. (1985) A rapid method for cloning and sequencing variable-region genes of expressed immunoglobulins. Gene 54, 167.
5. Maloney, D.G., Kaminski, M.S., Burowski, D., Haimovich, J. and Levy, R. (1985) Monoclonal anti-idiotype antibodies against the murine B cell lymphoma 38C13: characterization and use of probes for the tumor in vivo and in vitro. Hybridoma 4, 4479.
6. Müller, S., Wang, H.-T., Kaveri, S., Chattoppadhyay, S. and Köhler, H. (1991) Generation and specificity of monoclonal anti-idiotypic antibodies against human HIV-specific antibodies. J. Immunol. 147, 933.
7. Murano, R., Kuroki, M., Wunderlich, D., Poole, D.J., Colcher, D., Thur, A., Greiner, J.W., Simpson, J.F., Molinolo, A., Noguchi, P. and Schlom, J. (1983) Generation and characterization of B.73.3 second generation monoclonal antibodies reactive with the tumor-associated glycoprotein p 72 antigen. Cancer Res. 48, 736.
8. Paganelli, G., Belloni, C., Magnani, P., Zito, F., Pasini, A., Sassi, I., Meroni, M., Mariani, M., Vignali, M., Siccardi, A.G. and Fazio, F. (1992) Two-step tumor targeting in ovarian cancer patients using biotinylated monoclonal antibodies and radioactive streptavidin. Fur. J. Nucl. Med. 19, 322.
9. Potter, R.L. and Haley, B.E. (1983) Photoaffmity labeling of nucleotide binding sites with 8-azidopurine analogs: techniques and applications. Methods Enzymol. 91, 13.
10. Rajagopalan, K., Chavan, A.J., Haley, B. and Watt, D.S. (1993) Synthesis amd application of bidentate photoaffinity crosslinking reagents: Nucleotide photoaffinity probes with two photoreactive groups. J. Biol. Chem. 268, 14230.
11. Rajagopalan, K., Pavlinkova, G., Levy, S., Pokkuluri, P., Schiffer, M., Haley, B.E. and Köhler, H. (1996) Novel unconventional binding site in the variable region of immunglobulins. Proc. Natl. Acad. Sci. USA 93, 6019.
12. Salvucci, M., Chavan, A. and Haley, B., E. (1972) Identifation of peptides for the adenine binding domains of ATP and AMP in adenylate kinase: Isolation of photoaffinity labeled peptides by metal chelate chromatography. Biochemistry 31, 4479.
13. Schiffer, M., Girling, R.L., Ely, K.R. and Edmundson, A.B. (1973) Structure of a lambda-type Bence-Jones protein at 3.5-A resolution. Biochemistry 12, 4620.
14. Slavin-Chiorini, D.C., Kahmiri, S.V., Schlom, J. Calvo, B., Shu, L.M., Schott, M.E., Milenic, D.E., Snoy, P., Carrasquillo, J. and Anderson, K. (1995) Biological properties of chimeric domain-deleted anticarcinoma immunoglobulins. Cancer Res. 55, 5957.
15. Wilcheck, M. and Bayer, E.A. (1990) Avidin-Biotin Technology. Methods in Enzymology, Vol. 84. Accademic Press, New York.
16. Wong, S.S. (1991) Chemistry of Protein Conjugation and Crosslinking. CRC Press, Boca Raton, FL, 1.