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10,20 for 2'-deoxy-Dnucleoside phosphoramidites allowed assembling of L-enantiomeric domains. Deoxy-2'-L-thymine-loaded CPG was used for the synthesis of oligodeoxynucleotides having a L-domain at their 3'-end.
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The circular dichroism spectrum of D1L2 at 13 °C exhibits very weak signals between 220 and 320 nm which could result from the presence of two enantio-domains having opposite handedness and producing opposite signals. A non-zero C.D. spectrum may arise from absence of true symmetry in terms of sequence and orientation of each domain.
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