Quantitation of ERCC-2 gene expression in human tumor cell lines by reverse transcription-polymerase chain reaction in comparison to Northern blot analysis

Analytical Biochemistry

Volumn 244, Issue 1, 1997, Pages 50-54

  Chen, Zhong Ping ^a   Malapetsa, Areti ^a   Mohr, Gérard ^a   Brien, Susan ^a   Panasci, Lawrence C ^a  

^a MCGILL UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CELL CULTURE; METEOROLOGICAL INSTRUMENTS; PROTEINS; RNA; TRANSCRIPTION; TUMORS;

ANTICANCER DRUG; CHEMOTHERAPEUTIC AGENTS; HUMAN TUMOR CELL LINES; NORTHERN BLOT ANALYSIS; NUCLEOTIDE EXCISION REPAIR; PCR AMPLIFICATION; REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION; SENSITIVE METHOD;

POLYMERASE CHAIN REACTION;

AGAROSE; ANTINEOPLASTIC AGENT; BETA ACTIN; COMPLEMENTARY DNA; DNA; DNA FRAGMENT; NUCLEOTIDE; RNA;

ARTICLE; DENSITOMETRY; DNA DAMAGE; DRUG RESISTANCE; EXCISION REPAIR; GENE; GENE EXPRESSION; HUMAN; HUMAN CELL; NORTHERN BLOTTING; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TUMOR CELL LINE;

EID: 0031014650     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1996.9825     Document Type: Article

References (16)

1. 1. Ma, L., Hoeijmakers, J. H. J., and van der Eb, A. J. (1995) Biochim. Biophys. Acta 1242, 137-164.
2. 2. Aboussekhra, A., and Wood, R. D. (1994) Curr. Opin. Genet. Dev. 4, 212-220.
3. 3. Parker, R. J., Poirier, M. C., Bostick-Bruton, F., Vionnet, J., Bohr, V. A., and Reed, E. (1990) Basic Life Sci. 53, 251-261.
4. 4. Friedberg, E. C. (1992) Cell 71, 887-889.
5. 5. Drapkin, R., Sancar, A., and Reinberg, D. (1994) Cell 77, 9-12.
6. 6. Noë, A. J., Malapetsa, A., McQuillan, A. C., and Panasci, L. C. (1992) Proc. Am. Soc. Clin. Oncol. 11, 150.
7. 7. Chen, Z-P., Malapetsa, A., Marcantonio, D., Mohr, G., Brien, S., and Panasci, L. C. (1996) Cancer Res. 56, 2475-2478.
8. 8. Weber, C. A., Salazar, E. P., Stewart, S. A., and Thompson, L. H. (1990) EMBO J. 9, 1437-1447.
9. 9. Huynh, H., and Pollak, M. (1994) Cancer Res. 54, 3115-3119.
10. 10. Horikoshi, T., Danenberg, K. D., Stadlbauer, T. H. W., Volkenandt, M., Shea, L. C. C., Aigner, K., Gustavson, B., Leichman, L., Frösing, R., Ray, M., Gibson, N. W., Spears, C. P., and Danenberg, P. V. (1992) Cancer Res. 52, 108-116.
11. 11. Gilliland, D., Perrin, S., Blanchard, K., and Bunn, H. F. (1990) Proc. Natl. Acad. Sci. USA 87, 2725-2729.
12. 12. Kitzler, J., Hill, E., Hardman, R., Reddy, N., Philpot, R., and Eling, T. E. (1995) Arch. Biochem. Biophys. 316, 856-863.
13. 13. O'Leary, T. J., Tsai, M. M., Wight, C. F., and Cushion, M. T. (1995) J. Clin. Microbiol. 33, 718-724.
14. 14. Dabholkar, M. D., Berger, M. S., Vionnet, J. A., Egwuagu, C., Silber, J. R., Yu, J. J., and Reed, E. (1995) Cancer Res. 55, 1261-1266.
15. 15. Rodriguez, E., Carillon, C., Coquerel, A., Le Fur, G., Ferrara, P., Caput, D., and Shire, D. (1995) Mol. Brain Res. 29, 369-375.
16. 16. Gilliland, G., Perrin, S., and Bunn, H. F. (1990) in PCR Protocols: A Guide to Methods and Applications (Innis, M. A., Gelfand, D. H., Sninsky, J. J., and White, T. J., Eds.), pp. 60-69, Academic Press, San Diego.