Single cell analysis of intracellular osteopontin in osteogenic cultures of fetal rat calvarial cells

Journal of Cellular Physiology

Volumn 170, Issue 1, 1997, Pages 88-100

  Zohar, Ron ^a   Lee, Wilson ^a   Arora, Pamela ^a   Cheifetz, Sela ^a   McCulloch, Christopher ^a   Sodek, Jaro ^a  

^a UNIVERSITY OF TORONTO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

OSTEOPONTIN;

ANIMAL CELL; ARTICLE; BONE MATRIX; CALVARIA; CELL DIFFERENTIATION; CELL MIGRATION; CONFOCAL MICROSCOPY; FETUS; FLOW CYTOMETRY; NONHUMAN; OSSIFICATION; OSTEOBLAST; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; SKIN FIBROBLAST;

ANIMALS; ANTIBODIES, MONOCLONAL; BLOTTING, WESTERN; CELL CYCLE; CELL DIFFERENTIATION; CELL MOVEMENT; CELL SIZE; CELLS, CULTURED; CYTOKINES; CYTOPLASMIC GRANULES; DNA; FEMALE; FETUS; FIBROBLASTS; FLOW CYTOMETRY; GENE EXPRESSION; OSTEOGENESIS; OSTEOPONTIN; POLYMERASE CHAIN REACTION; PREGNANCY; RATS; RATS, WISTAR; RNA, MESSENGER; SIALOGLYCOPROTEINS; SKULL; STROMAL CELLS;

EID: 0031013634     PISSN: 00219541     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4652(199701)170:1<88::AID-JCP10>3.0.CO;2-K     Document Type: Article

References (35)

1. Aronow, M.A., Gerstenfeld, L.C., Owen, T.A., Tassinari, M.S., Stein, G.S., and Lian, J.B. (1990) Factors that promote progressive development of the osteoblast phenotype in cultured fetal rat calvaria cells. J. Cell. Physiol., 143:213-221.
2. Arora, P.D., and McCulloch, C.A.G. (1996) Dependence of fibroblast migration on actin severing activity of gelsolin. J. Biol. Chem. 271:20516-20523.
3. Aubin, J.E., Bellows, C.G., Turksen, K., Liu, F. and Heersche, J.N.M. (1992) Analysis of the osteoblast lineage and regulation of differentiation. In: Chemistry and Biology of Mineralized tissues. H. Slavkin and P. Price, eds. Elsevier Science Publishers, North-Holland, pp. 267-276.
4. Bellows, C.G., Aubin, J.E., Heersche, J.N.M., and Antosz, M.A. (1986) Mineralized bone nodules formed in vitro from enzymatically released rat calvaria cell populations. Calcif. Tissue Int., 38:143-154.
5. Brady, G., and Iscove, N.N. (1993) Construction of cDNA libraries from single cells. Methods Enzymol., 225:611-623.
6. Cantor, H. (1995) The role of Eta-1/osteopontin in the pathogenesis of immunological disorders. Ann. N. Y. Acad. Sci., 760:143-150.
7. Chen, J., Shapiro, H.S., and Sodek, J. (1992) Developmental expression of bone sialoprotein mRNA in rat mineralized connective tissues. J. Bone Miner. Res., 7:987-997.
8. Chen, J., McCulloch, C.A.G., and Sodek, J. (1993) Bone sialoprotein in developing porcine dental tissues: Cellular expression and comparison of tissue localization with osteopontin and osteonectin. Arch. Oral Biol., 38:241-249.
9. Darzynkiewicz, Z., Crissman, F., Traganos, F., and Steinkamp, J. (1982) Cell heterogeneity during the cell cycle. J. Cell. Physiol., 113:465-474.
10. Denhardt, D.T., and Guo, X. (1993) Osteopontin: A protein with diverse functions. FASEB J. 7:1475-1482.
11. Domenicucci, C., Goldberg, H.A., and Hofmann, T., Isenman, D., Wasi, S., and Sodek, J. (1988) Characterization of porcine osteonectin extracted from fetal calvariae. Biochem. J., 253:139-151.
12. Giachelli, C.M., Schwartz, S.M., and Liaw, L. (1995) Molecular and cellular biology of osteopontin. Trends Cardiovasc. Med., 5:88-95.
13. Ham, A.W., and Cormack, D.H. (1987) Bone. In: Ham's Histology. J.B. Lippincott Company, Philadelphia, 9th ed. pp. 273-319.
14. Hunter, G.K., and Goldberg, H.A. (1993) Nucleation of hydroxyapatite by bone sialoprotein. Proc. Natl. Acad. Sci. U. S. A., 90:8562-8565.
15. Ibaraki, K., Robey, P.G., and Young, M.F. (1993) Partial characterization of a novel 'GGA' factor which binds to the osteonectin promoter in bovine bone cells. Gene, 130:225-232.
16. Ko, S.D., Narayanan, A.S., and Page, R.C. (1981) Influence of cell cycle on collagen synthesis by human gingival fibroblasts. J. Periodont. Res., 16:302-308.
17. Lian, B.J., and Stein, G.S. (1992) Concepts of osteoblast growth and differentiation: Basis for modulation of bone cell development and tissue formation. Crit. Rev. Oral Biol. Med., 3:269-305.
18. Liaw, L., Skinner, M.P., Raines, E.W., Ross, R., Cheresh, D.A., Schwartz, S.M., and Giachelli, C.M. (1995) The adhesive and migratory effects of osteopontin are mediated via distinct cell surface integrins. J. Clin. Invest., 95:713-724.
19. Mark, M.P., Prince, C.W., Oosawa, T., Gay, S., Bronckers, A.L.J.J., and Butler, W.T. (1987) Immunohistochemical demonstration of 44-KD phosphoprotein in developing rat bones. J. Histochem. Cytochem., 35:707-715.
20. McCulloch, C.A.G., Strugurescu, M., Hughes, F., Melcher, A.H., and Aubin, J.E. (1991) Osteogenic progenitor cells in rat bone marrow stromal populations exhibit self-renewal in culture. Blood, 77:1906-1911.
21. McKee, M.D., and Nanci, A. (1995) Osteopontin and the bone remodeling sequence. Colloidal-gold immunocytochemistry of an interfacial extracellular matrix protein. Ann. N.Y. Acad. Sci., 760:177-189.
22. 2HS-glycoprotein) proteins in rat bone. J. Bone Miner. Res., 8:485-496.
23. 3 integrin stimulates immediate cell signais in osteoclasts. J. Biol. Chem., 266:20369-20374.
24. Oldberg, A., Franzen, A., and Heinegärd, D. (1986) Cloning and sequence analysis of rat bone sialoprotein (osteopontin) cDNA reveals an Arg-Gly-Asp cell-binding sequence. Proc. Natl. Acad. Sci. U. S. A., 83:8819-8823.
25. Owen, M. (1988) Marrow stromal stem cells. J. Cell Sci., S10:63-76.
26. Owen, T.A., Aronow, M., Shalhoub, V., Barone, L.M., Wilming, L., Tassinari, M.B.K., Shirwin, P., Lian, B.J., and Stein, G.S. (1990) Progressive development of the rat osteoblast phenotype in vitro: Reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix. J. Cell. Physiol., 745:420-430.
27. Patarca, R., Saavedra, R.A., and Cantor, H. (1993) Molecular and cellular basis of genetic resistance to bacterial infection: The role of the early T-lymphocyte activation-1/osteopontin gene. Crit. Rev. Immunol., 13:225-246.
28. Pinero, G.J., Farach-Carson, M.C., Devoll, R.E., Aubin, J.E., Brunn, J.C., and Butler, W.T. (1995) Bone matrix proteins in osteogenesis and remodelling in the neonatal rat mandible as studied by immunolocalization of osteopontin, bone sialoprotein, alpha 2HS-glycoprotein and alkaline phosphatase. Arch. Oral Biol., 40:145-155.
29. Senger, D.R., Peruzzi, C.A., Papadopoulos, A., and Tenen, D.G. (1989) Purification of a human milk protein closely similar to tumor secreted phosphoprotein and osteopontin. Biochim. Biophys. Acta, 996:43-48.
30. Sodek, J., Chen, J., Nagata, T., Kasugai, S., Todescan, R., Jr., Li, W.S.L., and Kim, R.H. (1995) Regulation of osteopontin expression in osteoblasts. Ann. N.Y. Acad. Sci., 760:223-241.
31. Tsukita, S., Oishi, K., Sato, N., Sagara, J., and Kawai, A. (1994) ERM family members as molecular linkers between the cell surface glycoprotein CD44 and actin based cytoskeletons. J. Cell Biol., 126:391-401.
32. Weber, G.F., Ashkar, S., Glimcher, M.J., and Cantor, H. (1996) Receptor-ligand interaction between CD44 and osteopontin (Eta-1). Science, 277:509-512.
33. Wrana, J.L., Kubota, T., Zhang, Q., Overall, C.M., Aubin, J.E., Butler, W.T., and Sodek, J. (1991) Regulation of transformation-sensitive secreted phosphoprotein (SPP-1, osteopontin) expression by transforming growth factor-β; comparisons with SPARC expression. Biochem. J., 273:523-531.
34. Yao, K.L., Todescan, R., Jr., and Sodek, J. (1994) Temporal changes in matrix protein synthesis and mRNA expression during mineralized tissue formation by adult rat bone marrow cells in culture. J. Bone Miner. Res., 9:231-240.
35. Yue, T.L., McKenna, P.J., Ohlstein, E.H., Farach-Carson, M.C., Butler, W.T., Johanson, K., McDevitt, P., Feuerstein, G.Z., and Stadel, J.M. (1994) Osteopontin stimulated vascular smooth muscle cell migration is mediated by β3 integrin. Exp. Cell Res., 274:459-464.