Reduction of background in protein kinase assays by electroblotting

BioTechniques

Volumn 22, Issue 1, 1997, Pages 74-78

  Enke, Deborah A ^a   Solomon, Mark J ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; CYCLIN DEPENDENT KINASE; PHOSPHORUS 32; PROTEIN KINASE;

ARTICLE; AUTORADIOGRAPHY; CHEMICAL REACTION; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME PHOSPHORYLATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; RADIOACTIVITY;

EID: 0031013303     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97221bm15     Document Type: Article

References (9)

1. Connell-Crowley, L., M.J. Solomon, N. Wei and J.W. Harper. 1993. Phosphorylation independent activation of human cyclin-dependent kinase 2 by cyclin A in vitro. Mol. Biol. Cell 3:79-92.
2. Moos, M. Jr., N.Y. Nguyen and T.Y. Liu. 1988. Reproducible high yield sequencing of proteins electrophoretically separated and transferred to an inert support. J. Biol. Chem. 263:6005-6008.
3. Pines, J. 1995. Cyclins and cyclin-dependent kinases: a biochemical review. Biochem. J. 308:697-711.
4. Smith, D.B. and K.S. Johnson. 1988. Single step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67:31-40.
5. Solomon, M.J. 1993. Activation of the various cyclin/cdc2 protein kinases. Curr. Opin. Cell Biol. 5:180-186.
6. cdc2-activating kinase. Trends Biochem. Sci. 19:496-500.
7. cdc2. Cell 63:1013-1024.
8. MO15. EMBO J. 12:3133-3142.
9. Volonté, C., R.A. Nichols and L.A. Greene. 1992. Rapid measurement of protein kinase and phosphatase activity by slot-filtration. BioTechniques 12:854-863.