High-fidelity PCR amplification of infectious copies of the complete simian virus 40 genome from plasmids and virus-infected cell lysates

Gene

Volumn 184, Issue 2, 1997, Pages 189-195

  Lednicky, John A ^a   Jafar, Sanjeeda ^a   Wong, Connie ^a   Butel, Janet S ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

Long PCR; Molecular clone; SV40 large tumor antigen; Viral enhancer; Viral plaque; Viral regulatory region; Viral titer

Indexed keywords

DNA POLYMERASE; DOUBLE STRANDED DNA; VIRUS DNA;

ARTICLE; CELL LYSATE; GENE AMPLIFICATION; MOLECULAR RECOGNITION; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SIMIAN VIRUS 40; VIRUS GENOME;

BASE SEQUENCE; CELL LINE; CLONING, MOLECULAR; DNA, VIRAL; GENOME, VIRAL; MOLECULAR SEQUENCE DATA; PHENOTYPE; PLAQUE ASSAY; PLASMIDS; POLYMERASE CHAIN REACTION; SIMIAN VIRUS 40; TEMPLATES, GENETIC;

EID: 0031012987     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(96)00594-X     Document Type: Article

References (19)

1. Agostini, H.T. and Stoner, G.L. (1995) Amplification of the complete polyomavirus JC genome from brain, cerebrospinal fluid and urine using pre-PCR restriction enzyme digestion. J. NeuroVirol. 1, 316-320.
2. Barnes, W.M. (1994) PCR amplification of up to 35-kb DNA with high fidelity and high yield from λ bacteriophage templates. Proc. Natl. Acad. Sci. USA 91, 2216-2220.
3. Cheng, S., Fockler, C., Barnes, W.M. and Higuchi, R. (1994) Effective amplification of long targets from cloned inserts and human genomic DNA. Proc. Natl. Acad. Sci. USA 91, 5695-5699.
4. Cohen, J. (1994) 'Long PCR' leaps into larger DNA sequences. Science 263, 1565-1566.
5. Fiers, W., Contreras, R., Haegeman, G., Rogiers, R., Van de Voorde, A., Van Heuverswyn, H., Van Herreweghe, J., Volckaert, G. and Ysebaert, M. (1978) Complete nucleotide sequence of SV40 DNA. Nature 273, 113-120.
6. Hirt, B. (1967) Selective extraction of polyoma DNA from infected mouse cell cultures. J. Mol. Biol. 26, 365-369.
7. Ilyinskii, P.O., Daniel, M.D., Horvath, C.J. and Desrosiers, R.C. (1992) Genetic analysis of simian virus 40 from brains and kidneys of macaque monkeys. J. Virol. 66, 6353-6360.
8. Innis, M.A., Myambo, K.B., Gelfand, D.H. and Brow, M.A.D. (1988) DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA. Proc. Natl. Acad. Sci. USA 85, 9436-9440.
9. Lednicky, J. and Folk, W.R. (1992) Two artificial Sp1-binding sites functionally substitute for the 21-base-pair repeat region to activate simian virus 40 growth in CV-1 cells. J. Virol. 66, 6379-6390.
10. Lednicky, J.A., Wong, C. and Butel, J.S. (1995a) Artificial modification of the viral regulatory region improves tissue culture growth of SV40 strain 776. Virus Res. 35, 143-153.
11. Lednicky, J.A., Garcea, R.L., Bergsagel, D.J. and Butel, J.S. (1995b) Natural simian virus 40 strains are present in human choroid plexus and ependymoma tumors. Virology 212, 710-717.
12. Lednicky, J.A. and Butel, J.S. (1996) Evolution of natural isolates of SV40 during tissue culture adaptation: changes occur in viral regulatory region but not in carboxy terminal domain of large T-antigen (submitted).
13. Noonan, C.A. and Butel, J.S. (1978) Temperature-sensitive mutants of simian virus 40. I. Isolation and preliminary characterization of B/C gene mutants. Intervirology 10, 181-195.
14. Reddy, V.B., Thimmappaya, B., Dhar, R., Subramanian, K.N., Zain, B.S., Pan, J., Ghosh, P.K., Celma, M.L. and Weissman, S.M. (1978) The genome of simian virus 40. Science 200, 494-502.
15. Stewart, A.R., Lednicky, J.A., Benzick, U.S., Tevethia, M.J. and Butel, J.S. (1996) Identification of a variable region at the carboxy terminus of SV40 large T-antigen. Virology 221, 355-361.
16. Stinebaugh, S. and Melnick, J.L. (1962) Plaque formation by vacuolating virus, SV40. Virology 16, 348-350.
17. Sweet, B.H. and Hilleman, M.R. (1960) The vacuolating virus, SV40. Proc. Soc. Exp. Biol. Med. 105, 420-427.
18. Takemoto, K.K., Kirschstein, R.L. and Habel, K. (1966) Mutants of simian virus 40 differing in plaque size, oncogenicity, and heat sensitivity. J. Bacteriol. 92, 990-994.
19. Tindall, K.R. and Kunkell, T.A. (1988) Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase. Biochemistry 27, 6008-6013.