Improved method for diagnosis of polycythemia vera based on flow cytometric analysis of autonomous growth of erythroid precursors in liquid culture

American Journal of Hematology

Volumn 54, Issue 1, 1997, Pages 47-52

  Manor, Daphna ^a   Rachmilewitz, Eliezer A ^a   Fibach, Eitan ^a  

^a HADASSAH HEBREW UNIVERSITY MEDICAL CENTER   (Israel)

Author keywords

Erythroid cells; Erythropoietin; Flow cytometry; Polycythemia

Indexed keywords

ERYTHROPOIETIN;

ARTICLE; CELL CULTURE; CELL GROWTH; CLINICAL ARTICLE; CONTROLLED STUDY; DIAGNOSTIC TEST; ERYTHROID CELL; FLOW CYTOMETRY; HUMAN; HUMAN CELL; POLYCYTHEMIA VERA; PRIORITY JOURNAL; STEM CELL;

CELLS, CULTURED; CULTURE MEDIA; ERYTHROID PROGENITOR CELLS; FLOW CYTOMETRY; HUMANS; LEUKOCYTES, MONONUCLEAR; POLYCYTHEMIA VERA;

EID: 0031012333     PISSN: 03618609     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1096-8652(199701)54:1<47::AID-AJH7>3.0.CO;2-4     Document Type: Article

References (16)

1. Casadevall N, Vainchenker W, Lacombe C, Vinci G, Chapman J, Breton-Gorius J, Varet B: Erythroid progenitors in polycythemia vera: Demonstration of their hypersensitivity to erythropoietin using serum-free culture. Blood 59:447, 1982.
2. Prchal JF, Axelrad AA: Bone marrow responses in polycythemia vera. New Engl J Med 290:1382, 1974.
3. Fibach E, Manor D, Oppenheim A, Rachmilewitz EA: Proliferation and maturation of human erythroid progenitors in liquid culture. Blood 73:100, 1989.
4. Weinberg RS, Thomson JC, Lao R, Chen G, Alter BP: Stem cell factor amplifies newborn and sickle erythropoiesis in liquid culture. Blood 81:2591, 1993.
5. Shintani N, Kohgo Y, Kato J, Kondo H, Fujikawa K, Miyazaki E, Niitsu Y: Expression and extracellular release of transferin receptors during peripheral erythroid progenitor cell differentiation in liquid culture. Blood 83:1209, 1994.
6. Fibach E, Kollia P, Schechter AN, Noguchi CT, Rodgers GP: Hemin-induced acceleration of hemoglobin production in immature cultured erythroid cells: Preferential enhancement of fetal hemoglobin. Blood 85:2967, 1995.
7. Berlin NI: Diagnosis and classification of the polycythemias. Semin Hematol 12:339, 1975.
8. Zanjani ED, Lutton JD, Hoffman R, Wasserman LF: Erythroid colony formation by polycythemia vera bone marrow in vitro. Dependence on erythropoietin. J Clin Invest 59:841, 1977.
9. Eaves CJ, Eaves AC: Erythropoietin (Epo) dose-response curves for three classes of erythroid progenitors in normal human marrow and in patients with polycythemia vera. Blood 52:1196, 1978.
10. Fibach E, Manor D, Treves A, Rachmilewitz EA: Growth of normal erythroid progenitors in liquid culture: A comparison with colony growth in semisolid culture. Int J Cell Cloning 9:57, 1991.
11. Broxmeyer HE, Cooper S, Lu L, Hangoc G, Handerson D, Cosman D, Lyman SD, William DE: Effect of murine mast cell growth factor (c-kit proto oncogene ligand) on colony formation by human marrow hematopoietic progenitor cells. Blood 77:2142, 1991.
12. Xiao M, Leemhuis T, Broxmeyer HE, Lu L: Influence of combinations of cytokines on proliferation of isolated single cell sorted human bone marrow hematopoietic progenitor cells in the absence and presence of serum. Exp Hematol 20:276, 1992.
13. Muller EW, de Wolf JTM, Hendriks DW, Esselink MT, Halie MR, Vellenga E: Recombinant human mast cell growth factor supports erythroid colony formation in polycytemia vera in the presence and absence of erythropoietin and serum. Exp Hematol 21:1353, 1993.
14. Issaad C, Vainchenker W: Growth of erythroid colonies in chronic myelogenous leukemia is independent of erythropoietin only in the presence of steel factor. Blood 84:3447, 1994.
15. Papayannopoulou T, Brice M, Blau CA: Kit ligand in synergy with Interleukin-3 amplifies the erythropoietin-independent globin synthesizing progeny of normal burst-forming units erythroid in suspension cultures: Physiological implications. Blood 81:299, 1993.
16. Streichman S, Avdi N, Joffe G, Matathias L, Tatarsky I: Some characteristics of circulating erythrocytes in polycythemia vera: Common features with normal young and foetal red blood cells. Scand J Haematol 36:33, 1986.