Differential localization of δ glutamate receptors in the rat cerebellum: Coexpression with AMPA receptors in parallel fiber-spine synapses and absence from climbing fiber-spine synapses

Journal of Neuroscience

Volumn 17, Issue 2, 1997, Pages 834-842

  Landsend, Alf Sommer ^a   Amiry Moghaddam, Mahmood ^a   Matsubara, Atsushi ^b   Bergersen, Linda ^a   Usami, Shin Ichi ^b   Wenthold, Robert J ^c   Ottersen, Ole P ^a  

^a UNIVERSITY OF OSLO   (Norway)

^b HIROSAKI UNIVERSITY   (Japan)

^c NATIONAL INSTITUTE ON DEAFNESS AND OTHER COMMUNICATION DISORDERS   (United States)

Author keywords

cerebellum; colocalization; freeze substitution; glutamate receptor; immunogold; synapse

Indexed keywords

ALPHA AMINO 3 HYDROXY 5 METHYL 4 ISOXAZOLEPROPIONIC ACID; AMPA RECEPTOR; GLUTAMATE RECEPTOR; GLUTAMIC ACID;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELLULAR DISTRIBUTION; FREEZE DRYING; IMMUNOGOLD STAINING; INTERNEURON; MALE; NONHUMAN; PARTICLE SIZE; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PURKINJE CELL; RAT; SYNAPSE;

EID: 0031012268     PISSN: 02706474     EISSN: None     Source Type: Journal    
DOI: 10.1523/jneurosci.17-02-00834.1997     Document Type: Article

References (24)

1. Araki K, Meguro H, Kushiya E, Takayama C, Inoue Y, Mishina M (1993) Selective expression of the glutamate receptor channel delta 2 subunit in cerebellar Purkinje cells. Biochem Biophys Res Commun 197:1267-1276.
2. Baude A, Nusser Z, Roberts JOB, Mulvihill E, Mcllhinney RAJ, Somogyi P (1993) The metabotropic glutamate receptor (mGluR1α) is concentrated at perisynaptic membrane of neuronal subpopulations as detected by immunogold reaction. Neuron 11:771-787.
3. Baude A, Nusser Z, Molnár E, Mcllhinney RAJ, Somogyi P (1995) High-resolution immunogold localization of AMPA-type glutamate receptor subunits at synaptic and nonsynaptic sites in the rat hippocampus. Neuroscience 69:1031-1055.
4. Bennet JA, Dingledine R (1995) Topology profile for a glutamate receptor: three transmembrane domains and a channel-lining reentrant membrane loop. Neuron 14:373-384.
5. Chaudhry FA, Lehre KP, van Lockeren Campagne M, Ottersen OP, Danbolt NC, Storm-Mathisen J (1995) Glutamate transporters in glial plasma membranes: highly differentiated localizations revealed by quantitative ultrastructural immunocytochemistry. Neuron 15:711-720.
6. Chittajallu R, Vignes M, Dev KK, Barnes JM, Collingridge GL, Henley JM (1996) Regulation of glutamate release by presynaptic kainate receptors in the hippocampus. Nature 379:79-81.
7. Gundersen HJG, Bendtsen TF, Korbo L, Marcussen N, Moller A, Nielsen K, Nyengaard JR, Pakkenberg B, Sorensen FB, Vesterby A, West MJ (1988) Some new, simple, and efficient stereological methods and their use in pathological research and diagnosis. APMIS 96:379-394.
8. Hayashi Y, Momiyama A, Takahashi T, Ohishi H, Ogawa-Meguro R, Shigemoto R, Mizuno N, Nakanishi S (1993) Role of metabotropic glutamate receptor in synaptic modulation in the accessory olfactory bulb. Nature 366:687-690.
9. Hirano T, Kasono K, Araki K, Shinozuka K, Mishina M (1994) Involvement of the glutamate receptor delta 2 subunit in the long-term depression of glutamate responsiveness in cultured rat Purkinje cells. Neurosci Lett 182:172-176.
10. Hirano T, Kasono K, Araki K, Mishina M (1995) Suppression of LTD in cultured Purkinje cells deficient in the glutamate receptor delta 2 subunit. NeuroReport 6:524-526.
11. Hjelle OP, Chaudhry FA, Ottersen OP (1994) Antisera to glutathione: characterization and immunocytochemical application to the rat cerebellum. Eur J Neurosci 6:794-804.
12. Hollmann M, Maron C, Heinemann S (1994) N-Glycosylation site tagging suggests a three transmembrane domain topology for the glutamate receptor GluR1. Neuron 13:1331-1343.
13. Ito M (1989) Long-term depression. Annu Rev Neurosci 12:85-102.
14. Kashiwabuchi N, Ikeda K, Araki K, Hirano T, Shibuki K, Takayama C, Inoue Y, Kutsuwada T, Yagi T, Kang Y, Aizawa S, Mishina M (1995) Impairment of motor coordination, Purkinje cell synapse formation, and cerebellar long-term depression in GluRδ2 mutant mice. Cell 81:245-252.
15. Kellenberger E, Hayat MA (1991) Some basic concepts for the choice of methods. In: Colloidal gold: principles, methods, and applications (Hayat MA, ed), pp 1-30. San Diego: Academic.
16. Linden DJ (1994) Long-term synaptic depression in the mammalian brain. Neuron 12:457-472.
17. Lomeli H, Sprengel R, Laurie DJ, Köhr G, Herb A, Seeburg PH, Wisden W (1993) The rat delta-1 and delta-2 subunits extend the excitatory amino acid receptor family. FEBS Lett 315:318-322.
18. Matsubara A, Laake JH, Davanger S, Usami S-i, Ottersen OP (1996) Organization of AMPA receptor subunits at a glutamate synapse: a quantitative immunogold analysis of hair cell synapses in the rat organ of Corti. J Neurosci 16:4457-4467.
19. Mayat E, Petralia RS, Wang YX, Wenthold RJ (1995) Immunoprecipitation, immunoblotting, and immunocytochemistry studies suggest that glutamate receptor delta subunits form novel postsynaptic receptor complexes. J Neurosci 15:2533-2546.
20. Molnar E, McIlhinney RA, Baude A, Nusser Z, Somogyi P (1994) Membrane topology of the GluR1 glutamate receptor subunit: epitope mapping by site-directed anti-peptide antibodies. J Neurochem 63:683-693.
21. Nusser Z, Mulvihill E, Streit P, Somogyi P (1994) Subsynaptic segregation of metabotropic and ionotropic glutamate receptors as revealed by immunogold localization. Neuroscience 61:421-427.
22. Ottersen OP (1989) Quantitative electron microscopic immunocytochemistry of amino acids. Anat Embryol (Berl) 180:1-15.
23. Ottersen OP, Zhang N, Walberg F (1992) Metabolic compartmentation of glutamate and glutamine: morphological evidence obtained by quantitative immunocytochemistry in rat cerebellum. Neuroscience 46:519-534.
24. Palay SL, Chan-Palay V (1974) Cerebellar cortex: cytology and organization. Heidelberg: Springer.