Production of an extracellular trypsin-like protease by the fungal plant pathogen Verticillium dahliae

Canadian Journal of Microbiology

Volumn 43, Issue 3, 1997, Pages 227-233

  Dobinson, Katherine F ^a   Lecomte, Nicolas ^a,b   Lazarovits, George ^a  

^a AGRICULTURE AND AGRI FOOD CANADA   (Canada)

^b NONE   (France)

Author keywords

phytopathogen; proteinase; verticillium wilt; wilt fungi

Indexed keywords

PROTEINASE;

ARTICLE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; FUNGUS; PLANT; PRIORITY JOURNAL;

VERTICILLIUM DAHLIAE;

EID: 0031010830     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/m97-031     Document Type: Article

References (34)

1. Abraham, L.D., Roth, A., Saddler, J.N., and Breuil, C. 1993. Growth, nutrition, and proteolytic activity of the sap-staining fungus Ophiostoma piceae. Can. J. Bot. 71: 1224-1230.
2. Altschul, W.F., Gish, W., Miller, W., Myers, E.W., and Lipman, D.J. 1990. Basic local alignment search tool. J. Mol. Biol. 215: 403-410.
3. Ball, A.M., Ashby, A.M., Daniels, M.J., Ingram, D.S., and Johnstone, K. 1991. Evidence for the requirement of extracellular protease in the pathogenic interaction of Pyrenopeziza brassicae with oilseed rape. Physiol. Mol. Plant Pathol. 38: 147-161.
4. Bennett, J.W., and Lasure, L.L. 1991. Growth media. In More gene manipulations in fungi. Edited by J.W. Bennett and L.L. Lasure. Academic Press, Inc., San Diego, Calif. pp. 441-458.
5. Carpita, N.C., and Gibeaut, D.M. 1993. Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth. Plant J. 3: 1-30.
6. Cook, R.G. 1994. Amino acid sequence analysis of blotted proteins. In Protein blotting: a practical approach. Edited by B.S. Dunbar. Oxford University Press, Inc., New York. pp. 207-220.
7. Cooper, R.M. 1984. The role of cell wall-degrading enzymes in infection and damage. In Plant diseases: infection, damage, and loss. Edited by R.K.S. Wood and G.J. Ellis. Blackwell Scientific Publications, Oxford, England. pp. 13-27.
8. Dunaevskii, Y.E., Pavlikova, E.B., Belyakova, G.A., and Belozerskii, M.A. 1995. Physiological role of protease inhibitors in plants: two groups of active inhibitors in buckwheat seed. Mol. Biol. 29: 747-750.
9. Erlanger, B.F., Kokowsky, N., and Cohen, W. 1961. The preparation and properties of two new chromogenic substrates of trypsin. Arch. Biochem. Biophys. 95: 271-278.
10. Hancock, J.G., and Millar, R.L. 1965. Association of cellulolytic, proteolytic. and xylolytic enzymes with southern anthracnose, spring black stem, and stemphylium leaf spot of alfalfa. Phytopathology. 55: 356-360.
11. Hislop, E.C., Paver, J.L., and Keon, J.P.R. 1982. An acid protease produced by Monilinia fructigena in vitro and in infected apple fruits, and its possible role in pathogenesis. J. Gen. Microbiol. 128: 799-807.
12. Kuć, J. 1962. Production of extracellular enzymes by Cladosporium cucumerinum. Phytopathology, 52: 961-963.
13. Laemmli, E.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London), 227: 680-685.
14. Lambert, F., and Pujarniscle, S. 1984. Purification and properties of the proteinase produced in vitro by Verticillium dahliae. Can. J. Microbiol. 30: 1488-1493.
15. Lamport, D.T.A. 1969. The isolation and partial characterization of hydroxyproline-rich glycopeptides obtained by enzymatic degradation of primary cell walls. Biochemistry, 8: 1155-1163.
16. Merril, C.R., Goldman, D., Sedman, S.A., and Ebert, M.H. 1981. Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science (Washington, D.C.), 211: 1437-1438.
17. Mosolov, V. 1979. A specific inhibitor of Colletotrichum lindemuthianum protease from kidney bean (Phaseolus vulgaris) seeds. Planta, 144: 265-269.
18. Mosolov, V.V., Loginova, M.D., Fedurkina, N.V., and Benken, I.I. 1976. The biological significance of proteinase inhibitors in plants. Plant Sci. Lett. 7: 77-80.
19. Murphy, J.M., and Walton, J.D. 1996. Three extracellular proteases from Cochliobolus carbonum: cloning and targeted disruption of ALP1. Mol. Plant-Microbe Interact. 9: 290-297.
20. North, M.J. 1982. Comparative biochemistry of the proteinases of eucaryotic microorganisms. Microbiol. Rev. 46: 308-340.
21. Pegg, G.F. 1981. Biochemistry and physiology of pathogenesis. In Fungal wilt diseases of plants. Edited by M.E. Mace, A.A. Bell, and C.H. Beckman. Academic Press, New York. pp. 193-253.
22. Peng, J.H., and Black, L.L. 1976. Increased proteinase inhibitor activity in response to infection of resistant tomato plants by Phytophthora infestans. Phytopathology, 66: 958-963.
23. Porter, F.M. 1966. Protease activity in diseased fruits. Phytopathology, 56: 1424-1425.
24. Powers, J.C., and Harper, J.W. 1986. Inhibitors of serine proteinases. In Proteinase inhibitors. Edited by A.J. Barrett and G. Salveson. Elsevier Science Publishers BV, Amsterdam. pp. 55-152.
25. Puhalla, J.E., and Bell, A.A. 1981. Genetics and biochemistry of wilt pathogens. In Fungal wilt diseases of plants. Edited by M.E. Mace, A.A. Bell, and C.H. Beckman. Academic Press, New York. pp. 145-192.
26. Rauscher, M., Mendgen, K., and Deising, H. 1995. Extracellular proteases of the rust fungus Uromyces viciue-fubue. Exp. Mycol. 19: 26-34.
27. Ries, S.M., and Albersheim, P. 1973. Purification of a protease secreted by Colletotrichum lindemahianum. Phytopathology. 63: 625-629.
28. Robertsen, B. 1984. An alkaline extracellular protease produced by Cladosporium cucumerinum and its possible importance in the development of scab disease of cucumber seedlings. Physiol. Plant Pathol. 24: 83-92.
29. Ryan, C.A. 1973. Proteolytic enzymes and their inhibitors in plants. Annu. Rev. Plant Physiol. 24: 173-196.
30. Rypniewski, W.R., Hastrup, S., Betzel, Ch., Dauter, M., Dauter, Z., Papendorf, G., Branner, S., and Wilson, K.S. 1993. The sequence and X-ray structure of the trypsin from Fusarium oxysporum. Protein Eng. 6: 341-348.
31. Schnathorst, W.C. 1981. Life cycle and epidemiology of Verticillium. In Fungal wilt diseases of plants. Edited by M.E. Mace, A.A. Bell, and C.H. Beckman. Academic Press, New York. pp. 81-111.
32. Segers, R., Butt, T.M., Kerry, B.R., and Peberdy, J.F. 1994. The nematophagous fungus Verticillium chlamydosporium produces a chymoelastase-like protease which hydrolyses host nematode proteins in situ. Microbiology, 140: 2715-2723.
33. St. Leger, R.J., Joshi, L., Bidochka, M.J., and Roberts, D.W. 1995. Protein synthesis in Metarhizium anisopliae growing on host cuticle. Mycol. Res. 99: 1034-1040.
34. Urbanek, H., and Yirdaw, G. 1978. Acid proteases produced by Fusarium species in cultures and in infected seedlings. Physiol. Plant Pathol. 13: 81-87.