Differential staining of apoptotic nuclei in living cells: Application to macronuclear elimination in Tetrahymena

Journal of Histochemistry and Cytochemistry

Volumn 45, Issue 5, 1997, Pages 675-683

  Mpoke, Solomon S ^a   Wolfe, Jason ^a  

^a WESLEYAN UNIVERSITY   (United States)

Author keywords

acridine orange; apoptosis; chondrocytes; ciliate conjugation; Hoechst 33342; lysosomes; nuclear death

Indexed keywords

ACRIDINE ORANGE;

APOPTOSIS; ARTICLE; CARTILAGE CELL; LYSOSOME; MACRONUCLEUS; NONHUMAN; PRIORITY JOURNAL; STAINING; TETRAHYMENA;

AVES; CILIOPHORA; TETRAHYMENA;

EID: 0031010142     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215549704500505     Document Type: Article

References (44)

1. Abrams J, White K, Fessler L, Steller H (1993) Programmed cell death during Drosophila embryogenesis. Development 117:29-43
2. Ameisen JC, Estaquier J, Idziorek T, Debels F (1995) The relevance of apoptosis to AIDS pathogenesis. Trends Cell Biol 5:27-32
3. Barry MA, Eastman A (1993) Identification of deoxyribonuclease II as an endonuclease involved in apoptosis. Arch Biochem Biophys 300:440-450
4. Brown F, Tirone S, Wolfe J (1993) Early encounters of the repetitive kind. A prelude to cell adhesion in conjugating Tetrahymena thermophila. Dev Dyn 196:195-204
5. Bruckner P, Horler I, Mendler M, Houze Y, Winterhalter K, Eich-Bender S, Spycher M (1989) Induction and prevention of chondrocyte hypertrophy in culture. J Cell Biol 109:2537-2545
6. Columbano A (1995) Cell death: current difficulties in discriminating apoptosis from necrosis in the context of pathological processes in vivo. J Cell Biochem 58:181-190
7. Compton MM (1992) A biochemical hallmark of apoptosis: inter-nucleosomal degradation of the genome. Cancer Metastasis Rev 11:105-119
8. D'Herde K, De Pestel G, Roels F (1994) In situ end labeling of fragmented DNA in induced ovarian atresia. Biochem Cell Biol 72:573-579
9. Darzynkiewicz Z (1990) Simultaneous analysis of cellular RNA and DNA content. In Darzynkiewicz Z, Robinson J, Crissman H, eds. Methods in Cell Biology. Vol 41. San Diego, Academic Press, 401-420
10. Darzynkiewicz Z, Bruno S, Del Bino G, Gorczyca W, Hotz MA, Lassota P, Traganos F (1992) Features of apoptotic cells measured by flow cytometry. Cytometry 13:795-808
11. Darzynkiewicz Z, Gorczyca W, Lassota P, Traganos F (1993) Altered sensitivity of DNA in situ to denaturation in apoptotic cells. Ann NY Acad Sci 677:334-340
12. Davis MC, Ward JG, Herrick G, Allis CD (1992) Programmed nuclear death: apoptotic-like degradation of specific nuclei in conjugating Tetrahymena. Dev Biol 154:419-432
13. Fesus L, Davies PJA, Piacentini M (1991) Apoptosis: molecular mechanisms in programmed cell death. Eur J Cell Biol 56:170-177
14. Gavrieli Y, Sherman Y, Ben-Sasson S (1992) Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentaion. J Cell Biol 119:493-501
15. Gerchenson LE, Rotello RJ (1992) Apoptosis: a different type of cell death. FASEB J 6:2450-2455
16. Giloh H, Sedat JW (1982) Fluorescence microscopy: reduced photobleaching of rhodamine and fluorescent protein conjugates by N-propylgallate. Science 271:1252-1255
17. Gorczyca W, Gong J, Darzynkiewicz Z (1993) Detection of DNA strand breaks in individual apoptotic cells by the in situ terminal deoxynucleotidyl and nick translation assays. Cancer Res 53: 1945-1951
18. Gottlieb RA, Giesing HA, Zhu JY, Engler RE, Babior BM (1995) Cell acidification in apoptosis: granulocyte colony-stimulating factor delays programmed cell death in neutrophils by up-regulating the vacuolar H+-ATPase. Proc Natl Acad Sci USA 92: 5965-5968
19. Kaczanowski A (1992) Mutation affecting cell separation and macronuclear resorption during conjugation in Tetrahymena thermophila: early expression of the zygotic genotype. Dev Genet 13:58-65
20. Kapuscinski J, Darzynkiewicz Z (1984) Condensation of nucleic acids by intercalating aromatic cations. Proc Natl Acad Sci USA 81:7368-7372
21. Li J, Eastman A (1995) Apoptosis in an interleukin-2-dependent cytotoxic T lymphocyte cell line is associated with intracellular acidification. J Biol Chem 270:3203-3211
22. Lockshin R, Beaulaton J (1981) Cell death: questions for histochemists concerning the causes of the various cytological changes. Histochem J 13:659-666
23. Martindale DW, Allis CD, Bruns PB (1982) Conjugation in Tetrahymena thermophila. A temporal analysis of cytological stages. Exp Cell Res 140:227-236
24. Mignotte B, Guenal I, Petit PX, Vayssiere JL (1995) Apoptosis, senescence, aging and mitochondria. In Vellas P, Albarede JL, Garry PJ, eds, L'Annee Gerontologique. Vol 9. Paris, Serdi, 51-63
25. Mpoke S, Wolfe J (1996) DNA digestion and chromatin condensation during nuclear death in Tetrahymena. Exp Cell Res 225:357-365
26. Nanney D, Caughey P (1953) Mating type determination in Tetrahymena pyriformis. Proc Natl Acad Sci USA 23:378-385
27. Ohkuma S, Poole B (1981) Cytoplasmic vacuolation of mouse peritoneal macrophages and the uptake into lysosomes of weakly basic substances. J Cell Biol 90:656-664
28. Oppenheim RW (1991) Cell death during development of the nervous system. Annu Rev Neurosci 14:453-501
29. Peitsch MC, Polzar B, Stephan H, Crompton T, MacDonald HR, Mannherz HG, Tschopp J (1993) Characterization of the endogenous deoxyribonuclease involved in nuclear DNA degradation tion during apoptosis (programmed cell death). EMBO J 12:371-377
30. Perez-Sala D, Collado-Escobar D, Mollinedo F (1995) Intracellular alkalinization suppresses lovastatin-induced apoptosis in HL-60 cells through the activation of a pH-dependent endonuclease. J Biol Chem 270:6235-6242
31. Schwartzman R, Cidlowski J (1993) Apoptosis: the biochemistry and molecular biology of programmed cell death. Endocrinol Rev 14:133-151
32. Shiokawa D, Ohyama H, Yamada T, Takahashi K, Tanuma S (1994) Identification of an endonuclease responsible for apoptosis in rat thymocytes. Eur J Biochem 226:23-30
33. Smale G, Nichols NR, Brady DR, Finch CE, Horton WE (1995) Evidence for apoptotic cell death in Alzheimer's disease. Exp Neurol 133:225-230
34. Sun D, Jiang S, Zheng L, Ojcius D, Young J (1994) Separate metabolic pathways leading to DNA fragmentation and apoptotic chromatin condensation. J Exp Med 179:559-568
35. Weiske-Benner A, Eckert WA (1987) Differentiation of nuclear structure during the sexual cycle in Tetrahymena thermophila II. Degeneration and autolysis of macro- and micronuclei. Differentiation 34:1-12
36. White K, Grether M, Abrams J, Young L, Farrell K, Steller H (1994) Genetic control of programmed cell death in Drosophila. Science 264:677-683
37. Wibo M, Poole B (1974) Protein degradation in cultured cells II. The uptake of chloroquine by rat fibroblasts and the inhibition of cellular protein degradation and cathepsin B1. J Cell Biol 63:430-440
38. Wijsman J, Jonker R, Keijzer R, Van De Velde C, Cornelisse C, Van Dierendonck J (1993) A new method to detect apoptosis in paraffin sections: in situ end-labeling of fragmented DNA. J Histochem Cytochem 41:7-12
39. Wolfe J (1985) Cytoskeletal reorganization and plasma membrane fusion in conjugating Tetrahymena. J Cell Sci 73:69-85
40. Wolfe J, Mpoke S, Tirone S (1993) Cilia, ciliary concanavalin A-binding proteins, and mating recognition in Tetrahymena thermophila. Exp Cell Res 209:342-349
41. Wyllie AH, Kerr JFR, Currie AR (1980) Cell death: the significance of apoptosis. Int Rev Cytol 68:251-305
42. Yanagisawa-Shiota F, Sakagami H, Kuribayashi N, Iida M, Sakagami T, Takeda M (1995) Endonuclease activity and induction of DNA fragmentation in human myelogenous leukemic cell lines. Anticancer Res 15:259-266
43. Yasuda M, Umemura S, Osamura RY, Kenjo T, Tsutsumi Y (1995) Apoptotic cells in the human endometrium and placental villi: pitfalls in applying the TUNEL method. Arch Histol Cytol 58:185-190
44. Zelenin A (1966) Fluorescence microscopy of lysosomes and related structures in living cells. Nature 212:425-426